Novel nanofiber membrane and yarn support for trachea transplantation and method for manufacturing novel nanofiber membrane and yarn support

Abstract

The invention relates to a novel nanofiber membrane and yarn support for trachea transplantation and a method for manufacturing the novel nanofiber membrane and yarn support. The novel nanofiber membrane and yarn support is of an inner and outer bilayer structure, the inner layer is a nanofiber membrane, and the outer layer is nanofiber yarn. The method comprises the following steps of manufacturing the nanofiber membrane, manufacturing the nanofiber yarn and manufacturing the tubular nanofiber membrane and yarn support. A raw material for manufacturing the nanofiber membrane and the nanofiber yarn is poly(epsilon-caprolactone-co-lactide). The high-voltage electrostatic spinning technique is adopted for the method for manufacturing the nanofiber membrane, and the dynamic liquid electrostatic spinning technique is adopted for the method for manufacturing the nanofiber yarn. The novel electrostatically-spun nanofiber membrane and yarn bilayer support for trachea transplantation is beneficial to adhesion and proliferation of cells, enables cartilage cells to grow along fibers of the nanofiber yarn in an oriented mode, is used for constructing a transplantable tissue engineering trachea, can be used for trachea defect repairing, and provides a significant reference for facilitating trachea mucosa repairing, clinically reconstructing a defective trachea and reducing the number of postoperative complications of trachea reconstruction.

Description

technical field [0001] The invention relates to a stent in the medical field and a preparation method thereof, in particular to a novel nanofiber membrane / yarn stent for tracheal transplantation and a preparation method thereof. Background technique [0002] When the human body is affected by inflammation, trauma, tumor or other lesions and causes large-scale damage to the trachea, it is necessary to resect a long section of the trachea and repair the damaged trachea. The previous one-stage end-to-end anastomosis technique is only suitable for the repair of local defects. When the length of defect resection exceeds 6.0 cm, the anastomotic tension is high due to the direct anastomosis pulling the surrounding organs and tissues, which may easily cause serious complications such as anastomotic leakage. With the rapid development of tissue engineering technology, people have tried methods such as allogeneic transplantation, autologous tissue transplantation and artificial materi...

AI Technical Summary

Problems solved by technology

By using PLCL to construct an artificial trachea scaffold, planting cells under suitable conditions has achieved good results, and obtained a tissue-engineered trachea with good biological properties that can be used for transplantation. However, it is still difficult to simulate the physical characteristics of biological trachea by planting chondrocytes. has a big deficiency

[0005] The applicant found that the fibers of the PLCL fabric produced by ordinary electrospinning technology in previous studies were disordered and had no directionality, and the arrangement of chondrocytes growing along the direction of the fibers also had no directionality, so that the obtained tissue engineered trachea Although it has a certain supporting and protective effect, unlike the horizontal circular cartilage of the biological trachea, the tissue-engineered trachea cannot resist lateral forces, so compared with the biological trachea, the tissue-engineered trachea will always be narrowed or collapsed Case

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