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Preparation method and application of a photoelectrochemical sensor for in situ generation of cds mycotoxins

A mycotoxin and photoelectrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of complex sample pretreatment, large amount of sample required, and single target, and achieve shortened production time and high sensitivity and specific detection, the effect of a wide linear range

Active Publication Date: 2016-04-20
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these detection methods have disadvantages such as single target, complex sample pretreatment, cumbersome operation, large amount of sample required, and long time consumption, which cannot well meet the needs of quantitative analysis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation method and application of a photoelectrochemical sensor for in-situ generation of CdS mycotoxins

[0040](1) The conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water in sequence, and dried with nitrogen; 6 μL, 2 mg / mL ceria-doped reduced graphene oxide composite nanomaterial was dropped onto the conductive surface of the conductive glass, Dry at room temperature, calcine at 400°C for 30min, and cool to obtain ceria-doped reduced graphene oxide composite nanomaterial GS-CeO 2 Modified glass electrodes;

[0041] (2) In GS-CeO 2 On the modified glass electrode surface, add 5 μL, 0.1 μg / mL mycotoxin antibody solution dropwise, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;

[0042] (3) Continue to drop 3 μL of BSA solution with a mass fraction of 1% to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a ...

Embodiment 2

[0046] Example 2 Preparation method and application of a photoelectrochemical sensor for in-situ generation of CdS mycotoxins

[0047] (1) The conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water in sequence, and dried with nitrogen; 6 μL, 3 mg / mL ceria-doped reduced graphene oxide composite nanomaterial was dropped onto the conductive surface of the conductive glass, Dry at room temperature, calcine at 450°C for 45min, and cool to obtain ceria-doped reduced graphene oxide composite nanomaterial GS-CeO 2 Modified glass electrodes;

[0048] (2) In GS-CeO 2 On the modified glass electrode surface, add 5 μL, 0.5 μg / mL mycotoxin antibody solution dropwise, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;

[0049] (3) Continue to drop 3 μL of BSA solution with a mass fraction of 2% to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a...

Embodiment 3

[0053] Example 3 Preparation method and application of an in-situ generated CdS mycotoxin photoelectrochemical sensor

[0054] (1) The conductive glass was ultrasonically cleaned with acetone, ethanol and ultrapure water in sequence, and dried with nitrogen; 6 μL, 4 mg / mL ceria-doped reduced graphene oxide composite nanomaterial was dropped onto the conductive surface of the conductive glass, Dry at room temperature, calcine at 500°C for 60 minutes, and cool to obtain ceria-doped reduced graphene oxide composite nanomaterial GS-CeO 2 Modified glass electrodes;

[0055] (2) In GS-CeO 2 On the modified glass electrode surface, add 5 μL, 1 μg / mL mycotoxin antibody solution dropwise, rinse the electrode surface with ultrapure water, and dry it in a refrigerator at 4 °C;

[0056] (3) Continue to drop 3 μL of BSA solution with a mass fraction of 3% to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, and dry it in a refr...

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PUM

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Abstract

The invention relates to a preparation method and application of a CdS mycotoxin photoelectrochemical sensor generated in situ. This method specifically uses ceria-doped reduced graphene oxide as the antibody capture substrate, and its excellent conductivity and large specific surface area can effectively reduce the background signal. Using Cd2+ functionalized porous TiO2 nanoparticles as a hapten marker carrier, by directly dropping Na2S on the electrode surface, a narrow bandgap CdS with high photoelectric conversion rate is generated in situ, and the CdS is irradiated by an LED lamp with a visible wavelength to generate a photocurrent signal . The carrier TiO2 has a good energy band match with CdS, which can further improve the photoelectric conversion signal of CdS, so as to prepare ultra-sensitive detection of zearalenone, α-zearalenol, aflatoxin B1, B2, ochratoxin A, B Competitive photoelectrochemical immunosensors for various mycotoxins.

Description

technical field [0001] The invention relates to a preparation method and application of an in-situ generated CdS mycotoxin photoelectrochemical sensor, in particular to a preparation method and application of a competitive mycotoxin photoelectrochemical sensor that generates CdS in situ, and belongs to new functional materials and food safety detection technology field. Background technique [0002] In recent years, food pollution has become increasingly serious and frequent, which not only causes huge economic losses, but also seriously affects human health. Mycotoxin is one of the main food pollutants, it is a secondary metabolite produced by mold or fungus, due to its wide distribution, it is easy to contaminate crops, contaminated food or feed, and animals fed by the feed, etc. Enter the food chain and indirectly enter the human body, eventually causing serious consequences such as neurological and endocrine disorders, immunosuppression, liver and kidney damage, reprodu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N27/327
CPCG01N27/26G01N33/53G01N33/54346G01N33/5438
Inventor 魏琴黎荣霞杜斌马洪敏吴丹胡丽华
Owner UNIV OF JINAN
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