Use of WRY tripeptide in preparation of drug for treating Alzheimer's disease
A technology for Alzheimer's disease and drugs, applied in the field of medicine, can solve the problems of large drug side effects, ineffective effects, unclear drugs for Alzheimer's disease, etc.
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experiment example 1
[0019] Experimental example 1, Thioflavin T test of a WRY tripeptide for the prevention or treatment of Alzheimer's disease:
[0020] Aβ 1-42 Dissolve in pH7.4, the concentration is 0.01mol / L in the phosphate buffer solution of 0.01mol / L and make 50μmol / L mixed solution, Aβ in this mixed solution 1-42 The initial concentration of the peptide was 50 μmol / L, until the final concentration was 2.5 μmol / L, placed at 37 ° C for 48 h, then added ThT (5 μmol / L dissolved in 50 mmol / L glycine-NaOH solution, pH 8.50 ) to detect fluorescence at 450 nm and 485 nm. Each sample was measured 3 times and the fluorescence intensity was recorded.
[0021] combine figure 1 As shown, the results of Example 1 show that: Thioflavin T can rapidly interact with Aβ 1-42 Combined with the polymer fiber, a new light with a wavelength of 450nm is excited, and the light with a wavelength of 482nm is obviously enhanced, while WRY tripeptide and Aβ 1-42 Co-cultured WRY tripeptide-Aβ 1-42 The thiofl...
experiment example 2
[0022] Experimental example 2, A circular dichroism test of WRY tripeptide for preventing or treating Alzheimer's disease:
[0023] Aβ at a concentration of 50 μmol / L 1-42 Dissolve in phosphate buffer solution, and add WRY tripeptide at a concentration of 50 μmol / L to the experimental group, then place it at 37°C and incubate for 48 h, the Aβ in the mixed solution 1-42 and the final concentration of WRY tripeptide is 5μmol / L, using a container dish with a thickness of 1mm, Aβ 1-42 Monomeric and WRY tripeptide-Aβ 1-42 The complex samples were respectively prevented from detecting the structure in a circular dichroism spectrometer, and the spectra were recorded at 25°C with a wavelength of 190-260 nm and a wave width of 0.5 nm.
[0024] combine figure 2 As shown, the results of Example 2 show that the circular dichroism spectrum shows that the β sheet structure in the WRY tripeptide-Aβ complex will decrease, such as figure 2 As shown, circular dichroism detection of Aβ...
experiment example 3
[0025] Experimental example 3, A kind of WRY tripeptide for preventing or treating Alzheimer's disease transmission electron microscope (TEM) test:
[0026]Aβ with phosphate buffer (PBS, pH7.4) 1-42 The sample was dissolved to a concentration of 1 mg / ml, and then placed in the WRY tripeptide experimental group and the blank control group at a concentration of 25 μmol / L, and incubated for 48 hours until the final concentration of the mixed solution was 25 μmol / L, and 5 μl of the sample was applied Add 1% uranyl formate to the 300-hole Formvar-carbon copper grid, stain for 1 min, dry in the air, and then observe under the electron microscope to detect Aβ 1-42 and WRY tripeptide-Aβ 1-42 The structure of the complex.
[0027] combine image 3 Shown, embodiment 3 result shows: as image 3 As shown in the electron microscope image, from Aβ 1-42 Monomer, high-density long linear Aβ can be observed in the figure 1-42 fibers, the fibers aggregate into parallel bundles, and ...
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