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Construction method and applications of antibacterial bionic silicification collagen scaffold material

A technology of scaffold material and silicified collagen, applied in medical science, prosthesis, etc., can solve the problems of partial degradation of collagen, long cycle, limited calcification depth, etc., to improve mechanical properties, good mechanical properties, and promote adhesion Effect

Active Publication Date: 2015-01-28
焦凯
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current bionic calcification method still has problems such as long cycle, limited calcification depth, uneven calcification, partial degradation of collagen, and no antibacterial properties, which restrict the wide application of this method.

Method used

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  • Construction method and applications of antibacterial bionic silicification collagen scaffold material
  • Construction method and applications of antibacterial bionic silicification collagen scaffold material
  • Construction method and applications of antibacterial bionic silicification collagen scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The antibacterial biomimetic silicified collagen scaffold material construction method of this embodiment, according to the following steps:

[0042] (1) Preparation of collagen sponge scaffold: Take 500g of fresh bovine tendon, remove fascia, fat and other impurities and cut into thin slices; add the cut tendon slices to 250mL of 0.5% protease digestion solution, and digest at a constant temperature of 37°C After 3 hours of treatment, use 100mL0.3g / L H 2 o 2The solution terminates the enzyme digestion reaction, rinses repeatedly with distilled water and then air-dries; add 100mL of 0.1% acetic acid solution to 100g of dried tendons to swell, stir evenly and centrifuge to remove impurities; use phosphate solution for repeated salting-out to improve collagen The purity of the solution; use polyethylene glycol to concentrate to prepare a collagen solution with a final concentration of 20 mg / mL; after pre-cooling at -20°C for 2 hours, freeze-dry in a positive air freeze d...

Embodiment 2

[0061] This embodiment differs from Embodiment 1 in that:

[0062] (3) Tetraethyl orthosilicate is used as raw material at room temperature to prepare orthosilicic acid with a concentration of 5% by dilute acid hydrolysis; the obtained orthosilicic acid and 6% chlorhexidine gluconate are uniformly Mix and adjust the pH value of the final solution to 5.5; centrifuge at 3000 rpm for 3 minutes, and take the supernatant to obtain a 2.5% orthosilicate precursor solution stabilized with 3% chlorhexidine gluconate.

[0063] (4) Place the surface-treated collagen scaffold obtained in step (2) in the stable silicic acid precursor solution obtained in step (3) for mineralization treatment, replace the fresh silicic acid precursor solution every day, and incubate for 6 days respectively , rinsed repeatedly with distilled water, freeze-dried for later use, and prepared an antibacterial biomimetic silicified collagen scaffold material.

Embodiment 3

[0065] This embodiment differs from Embodiment 1 in that:

[0066] (3) Tetraethyl orthosilicate is used as raw material at room temperature to prepare orthosilicic acid with a concentration of 8% by dilute acid hydrolysis; the obtained orthosilicic acid and 10% chlorhexidine gluconate are uniformly Mix and adjust the pH value of the final solution to 5.5; centrifuge at 3000 rpm for 3 minutes, and take the supernatant to obtain a 4% orthosilicate precursor solution stabilized with 5% chlorhexidine gluconate.

[0067] (4) Place the surface-treated collagen scaffold obtained in step (2) in the stable silicic acid precursor solution obtained in step (3) for mineralization treatment, replace the fresh silicic acid precursor solution every day, and incubate for 2 days respectively , rinsed repeatedly with distilled water, freeze-dried for later use, and prepared a new type of antibacterial biomimetic siliconized collagen scaffold material.

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Abstract

The present invention relates to a construction method and applications of an antibacterial bionic silicification collagen scaffold material. The method comprises: treating a collagen sponge scaffold in a mixed solution of orthosilicic acid and an antibacterial agent to obtain the antibacterial bionic silicification collagen scaffold material, wherein preferably the collagen sponge scaffold is the collagen sponge scaffold being subjected to the surface treatment through polycation, and the antibacterial solution is the chlorhexidine digluconate solution or benzalkonium chloride. According to the present invention, the antibacterial agent chlorhexidine or benzalkonium chloride is integrated into the bionic silicification collagen material within fiber, such that the slow release of the antibacterial component and the degradation of the bone graft material are synchronized, and the novel silicification collagen scaffold material with characteristics of good mechanical property, good osteogenic property and antibacterial property is constructed; and the introduced antibacterial component chlorhexidine or benzalkonium chloride can be slowly released along with the release of the silicic acid in the material, such that the synchronization of the provision of the antibacterial effect and the degradation of the bone graft material is achieved.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffold materials, and relates to a construction method and application of an antibacterial bionic siliconized collagen scaffold material. Background technique [0002] With the increasing number of bone tissue defects caused by trauma, tumors, congenital deformities, etc., people's demand for bone graft materials is also increasing. According to data, at present, there are nearly 2.2 million bone graft operations worldwide every year, and the cost of bone graft operations reached nearly $2.5 billion. Infected bone defect and intraoperative and postoperative infection at the bone graft site have become one of the main reasons for bone graft failure. resulting in bone graft failure. Therefore, for a long time, the development of biomaterials with antibacterial function to replace autologous bone to repair bone defects has been an important topic in the field of medicine and materials science. H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/54A61L27/02A61L27/16A61L27/14
Inventor 焦凯牛丽娜陈吉华郑智明李齐宏柴治国
Owner 焦凯
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