Improved tomato juice culture medium and application thereof

Abstract

The invention relates to the field of microorganisms and in particular relates to an improved tomato juice culture medium and an application thereof. The improved tomato juice culture medium comprises the following components: a triple sugar iron (TSI) agar culture medium, 2.5g / L of tomato powder, 6g / L of sodium acetate, 2.0g / L of dipotassium phosphate, 0.5g / L of monopotassium phosphate and 0.6g / L of Tween 80, and pH value of the improved tomato juice culture medium is 6.6-7.0; and one litre of triple sugar iron (TSI) agar culture medium contains the following components: 15.0g of tryptone, 10.0g of beef extract powder, 5.0g of yeast powder, 4.0g of sodium chloride, 5.0g of proteose peptone, 20.0g of lactose, 1.0g of glucose, 10.0g of cane sugar, 0.2g of ferrous sulfate, 0.3g of sodium thiosulfate, 15.0g of agar, 0.04g of bromcresol purple and balance of distilled water. The improved tomato juice culture medium is simple in composition, easy to prepare and operate, low in cost and beneficial to popularization and application; bacteria grow quickly, and mutation rate is low; and observation and analysis are facilitated.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an improved tomato juice culture medium and its application. Background technique [0002] The diversity and variability among microorganisms in the world and the ecological complexity of species habitats have brought about the breadth, complexity and permanence of exploring and understanding microorganisms. A species of microorganism is a unique gene pool. The metabolism of microorganisms is an intricate life activity. In the long-term biological evolution, a complete and unified sensitive regulatory system has been formed. Depending on this regulatory system, various metabolic activities are strictly controlled. . Methodical and coordinated and adapts flexibly to circumstances. Different culture media, due to different media formulations, affect its growth and variation. [0003] Medium is an artificially prepared nutrient for the growth and maintenance of microorga...

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Problems solved by technology

[0006] At present, it is still not possible to infer and calculate the medium formula suitable for a certain strain from the basic principles of biochemical reactions. We can only use the basic theories of biochemistry, cell biology, microbiology, etc., and refer to the more suitable ones used by predecessors. The empirical formula of a certain type of strain, combined with the characteristics of the strains used and the product, uses small fermentation equipment such as shake flasks and glass tanks, and selects a more suitable medium according to certain experimental designs and experimental methods

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