Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mushroom powder rich in vitamin K2 and preparation method thereof

A technology of vitamin and mushroom powder, applied in the biological field, can solve the problems of no health care function and medicinal value, no cheap raw material vitamin K2, etc., achieve anti-cancer and repair damaged cell functions, prevent and treat osteoporosis, and prevent heart disease. Effects on Vascular Health

Active Publication Date: 2015-02-04
ZHEJIANG BAISHANZU BIOTECH CO LTD
View PDF1 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, domestic patents on the preparation of vitamin K2 mainly focus on: (1) strain mutagenesis screening; (2) purification of vitamin K2; there is no patent for the production of vitamin K2 using cheap raw materials, and there is no use of Grifola frondosa slag and shiitake mushroom slag Examples of vitamin K2 production using mushroom dregs that are used both as medicine and food, and have good health effects and medicinal value

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mushroom powder rich in vitamin K2 and preparation method thereof
  • Mushroom powder rich in vitamin K2 and preparation method thereof
  • Mushroom powder rich in vitamin K2 and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: strain screening

[0048] Dissolve 1g of natto product in 100ml of normal saline, oscillate and mix evenly, and dilute the sample suspension 10 times in order to prepare 10 -1 -10 -7 concentration, respectively, 10 -3 -10 -7 Draw 100ul of the dilutions from the five concentrations and add them to the screening medium for coating, apply three plates for each concentration, incubate for 48 hours at 37°C, select larger colonies that can produce hydrolysis circles, and continue to streak in the screening medium , to obtain pure bacteria, after fermentation and re-screening, high-performance liquid phase analysis and mass spectrometry identification, the bacterial strain Bacillus natto R capable of producing vitamin K2 was obtained, and the vitamin K2 produced by the bacterial strain was in the MK-7 configuration (such as figure 1 ).

[0049] In order to improve the ability of the bacteria to decompose protein, the obtained Bacillus natto R was subjected t...

Embodiment 2

[0052] Example 2: Investigation on the basic growth performance of bacterial strains.

[0053] The bacterial strain CCTCC NO:M 2014405 that screening obtains is carried out basic performance investigation, adopts different liquid seed culture medium to cultivate, investigates cell growth rate, and result is shown in Table 1, and used medium is (% represents the quality that contains substance in every 100ml medium g):

[0054] Medium 1: 100mL bean sprout juice, 5% sucrose

[0055] Medium 2: 1% glucose, 1% peptone, 0.5% NaCl

[0056] Medium 3: 3% glucose, 4% peptone, 0.5% NaCl, 0.5% beef extract, 0.5% yeast extract

[0057] Medium 4: 0.5% glucose, 1% peptone, 0.5% NaCl, 0.5% beef extract, 0.5% yeast extract

[0058] Table 1 The growth of bacteria in different seed media

[0059]

[0060]

[0061] From the above test results, except for the No. 2 medium, the other three can make the bacteria grow better, and the No. 3 medium is selected.

Embodiment 3

[0062] Example 3: Investigation on the ability of bacterial strains to accumulate vitamin K2.

[0063] Four different fermentation media were selected for fermentation, and the production capacity of vitamin K2 of the strain was preliminarily discussed. The culture temperature is 30° C., and the rotation speed is 120 rpm. (% represents the quality g of the substance contained in every 100ml medium):

[0064] Medium 1: 5% glycerol, 3% soybean peptone, 0.6g / L yeast powder, 0.3g / L K 2 HPO 4 , 0.1g / LCaCl 2 ·H 2 O, 0.3g / LMgSO 4 ·7H 2 o

[0065] Medium 2: 5% glycerol, 3% soybean powder, 0.6g / L yeast powder, 0.3mol / LK 2 HPO 4 , 0.1g / LCaCl 2 ·H2O, 0.3g / LMgSO 4 ·7H2O

[0066] Medium 3: 15g / L peptone, 25g / L yeast powder, 70g / L glycerol, 0.5g / L K 2 HPO 4 , pH7.3

[0067] Medium 4: 100mL soybean milk, 2% glucose, 0.5% NaCl, 2% NaOH to adjust pH7.0

[0068] Table 2 Comparison table of vitamin K2 produced by different fermentation media

[0069]

[0070] From the above t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses a mushroom powder rich in vitamin K2. The mushroom powder contains a plurality of nutrients, wherein the content of vitamin K2 is over 200ug / g. The mushroom powder also contains a plurality of proteins, dietary fibers and trace elements. The present invention also discloses a preparation method of the mushroom powder. According to the present invention, autonomously screened bacillus natto (preservation number CCTCC M2014405) is used as a starting strain, mushroom dregs extracted out of active polysaccharides are used as main nitrogen source, a large number of bacterial cells are bred through fermentation controlled by appropriate means. The mushroom powder rich in vitamin K2 is prepared through cell collection, membrane filtration or centrifugation, and spray drying. The mushroom powder has efficacy of treating and preventing osteoporosis, promoting cardiovascular health, repairing damaged cells, resisting tumor, and the like. The present invention establishes the foundation for industrial production of vitamin K2, comprehensive utilization of mushroom dregs and development of new health care foods, and provides a new approach for producing vitamin K2.

Description

technical field [0001] The invention relates to mushroom powder rich in vitamin K2 and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Vitamin K2 is a kind of fat-soluble blood coagulation vitamin, which is a series of menadione compounds. It has remarkable effects in the prevention and treatment of osteoporosis, and is called "the fourth generation of anti-osteoporosis products". Vitamin K2 is a class of compounds—menaquinone compounds. There are 14 forms according to the length of the isoprene side chain at the C-3 position of its molecular structure, represented by MK-n (n refers to the isoprene on the side chain. The number of pentadiene units), menadione-4 (MK-4) and menadione-7 (MK-7) are the most common, MK-4 has a similar molecular weight to vitamin K1, and both have a very short serum half-life, To achieve blood circulation requires a very large dose, 1000 times higher than the recommended dose of MK-7. MK-7 can...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23L1/28A23L31/00
CPCA23L31/00A23V2002/00A23V2200/306A23V2200/326A23V2200/302A23V2200/308
Inventor 黄和邱宏伟任路静王星丽
Owner ZHEJIANG BAISHANZU BIOTECH CO LTD
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com