Illumination-carbon dioxide united-regulation and control photo-bioreactor

A technology of photobioreactor and carbon dioxide, applied in the direction of photobioreactor, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc., which can solve the problems of hysteresis and passivity

Active Publication Date: 2015-02-04
中科榆林能源技术运营有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Illumination-carbon dioxide united-regulation and control photo-bioreactor

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Embodiment 1

[0033] Combustion calorific values ​​of several typical microalgae, including Zhanjiang Isochrysis (golden algae), Tetraphyllum (marine green algae), Chlorella (freshwater green algae) and Spirulina (cyanobacteria), using a combustion heat measurement device Measured, respectively: 2.2±0.08, 1.7±0.02, 2.0±0.03 and 2.1±0.01, unit x10 4 J / g.

[0034]Taking Isochrysis zhanjiang as the culture, measure the different culture systems, including under the fluorescent light source (the input light intensity of the culture system is 50-250 μ mol / (m 2 ·s)) 0.5L tubular airlift reactor, 1L plate reactor, 1.5L plate reactor, 3L shake flask, 30L plate reactor, 100L plate reactor and under natural light (the input light intensity of the culture system is 50-2000μmol / (m 2 ·s)) 100L plate reactor, the light energy utilization rate calculated based on the biomass increment to the total input light energy during harvesting is: 9.3%, 6.0%, 6.4%, 3.9%, 3.2%, 3.5% and 1.2% respectively .

Embodiment 2

[0036] Under the condition of standard 3x F / 2 medium, in the 500mL tubular photobioreactor, aerate according to the aeration rate of 100mL / min (the volume ratio of 2% CO 2 ), 14:10 light-to-dark ratio cultivates Isochrysis zhanjiang, and the aeration volume of the section without light is adjusted to 50% and 20% of the normal level respectively, and F is measured at the beginning and end points of the section without light v / F m Compared with the normal level, the 50% and 20% groups decreased by 1.0% and 7.5% respectively, and the ventilation volume of 20% was determined as the minimum ventilation volume. Among them, the low-ventilation biomass content was 0.423g / L, about 8.7% higher than that of the normal-ventilation group (0.389g / L).

Embodiment 3

[0038] On the basis of the culture conditions described in Example 2, on the basis of maintaining the constant air intake rate, using the control process of the present invention, a dynamic CO 2 Supplementary dinoflagellates from Zhanjiang were cultivated. The conditional culture described in Example 2 was used as a control. The culture results show that the CO in the system can 2 The total amount of feed decreased to less than 70% of the control group, and at the same time, the biomass increased by 5-40% compared with the control group, with an average increase ratio of 20%.

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Abstract

The invention relates to a novel photo-bioreactor, which, during a photoautotrophic cultivation process, is specifically used for implementing replenishment of carbon dioxide matched with light intensity by virtue of dynamic regulation of carbon dioxide by detecting changes of illumination intensity for photosynthesis and the concentration of liquid carbon dioxide in the system and calculating the replenishing speed of carbon dioxide. The photo-bioreactor disclosed by the invention is used for effectively guaranteeing the coordination of energy and material input in the photosynthesis and especially applicable to outdoor cultivation which uses natural light as a light source.

Description

Technical field: [0001] The invention belongs to a novel photobioreactor, which is applied to the photoautotrophic cultivation of microalgae, photosynthetic bacteria and the like. Background technique: [0002] Microalgae and photosynthetic bacteria can use their photosynthetic system to efficiently absorb solar energy and fix CO 2 The production of biomass has thus attracted the attention of bioengineering research and production, and is expected to be widely used in the production of bioenergy, bio-based materials and high-value products. Among them, photobioreactor cultivation is an important link in the development of the application of such biological resources, especially in the fields that require large-scale cultivation. [0003] In photobioreactors, the energy for biological growth comes from sunlight. Therefore, in the process of large-scale production, how to make full use of sunlight is the need to consider and make breakthroughs in the cultivation of photobiore...

Claims

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Application Information

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IPC IPC(8): C12M1/36C12M1/34C12M1/04C12N1/20C12N1/12C12R1/89
CPCC12M21/02C12M31/10C12M41/06
Inventor 薛松褚亚东曹旭鹏陆洪斌
Owner 中科榆林能源技术运营有限责任公司
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