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Nano lipidosome containing green tea extractive, and preparation method thereof

A technology of green tea extract and nano-liposome, which is applied in the chemical field to achieve the effects of good antioxidant stability, good elongation at break, and good antioxidant activity

Inactive Publication Date: 2015-03-04
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report of making green tea extract into nanoparticles and embedding them in liposomes to improve its oxidative stability

Method used

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  • Nano lipidosome containing green tea extractive, and preparation method thereof
  • Nano lipidosome containing green tea extractive, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A nanoliposome containing green tea extract, the raw material component contains green tea extract 5 wt .%.

[0027] (1) Weigh soybean lecithin and cholesterol with a mass ratio of 2:1 into a 250ml round bottom flask, add 40ml of chloroform to dissolve evenly, and evaporate the mixture under reduced pressure to the inner wall of the flask at 25°C to form a transparent film .

[0028] (2) Add 20ml of phosphate-buffered saline solution containing green tea extract, and continue to rotate until the membrane is completely dissolved.

[0029] (3) Sonicate the prepared liquid with a 50W probe for 8min, supersonicate for 3s, and stop for 4s to obtain the green tea extract nanoliposome suspension.

[0030] (4) Measure the average particle size, encapsulation efficiency and Zeta potential of the green tea extract nanoliposomes prepared in step (3).

[0031] After testing, the average particle size of the green tea extract nano-liposome prepared in this example is 150nm, ...

Embodiment 2

[0033] A kind of nano liposome containing green tea extract, the raw material component contains green tea extract 10 wt .%.

[0034] (1) Weigh soybean lecithin and cholesterol with a mass ratio of 3:1 in a 250ml round bottom flask, add 40ml of chloroform to dissolve evenly, and evaporate the mixture under reduced pressure to the inner wall of the flask at 30°C to form a transparent film .

[0035] (2) Add 25ml of phosphate-buffered saline solution containing green tea extract, and continue to rotate until the membrane is completely dissolved.

[0036] (3) Ultrasound the prepared liquid with a 100W probe for 10min, supersonic for 4s, and stop for 4s to obtain the green tea extract nanoliposome suspension.

[0037] (4) Measure the average particle size, encapsulation efficiency and Zeta potential of the green tea extract nanoliposomes prepared in step (3).

[0038] After testing, the average particle size of the green tea extract nano-liposome prepared in this example i...

Embodiment 3

[0040] A nanoliposome containing green tea extract, the raw material component contains green tea extract 15 wt .%.

[0041] (1) Weigh soybean lecithin and cholesterol with a mass ratio of 4:1 in a 250ml round bottom flask, add 40ml of chloroform to dissolve evenly, and evaporate the mixture under reduced pressure to the inner wall of the flask at 35°C to form a transparent film .

[0042] (2) Add 30ml of phosphate-buffered saline solution containing green tea extract, and continue to rotate until the membrane is completely dissolved.

[0043] (3) Ultrasound the prepared liquid with a 150W probe for 12min, supersonicate for 5s, and stop for 4s to obtain the green tea extract nanoliposome suspension.

[0044] (4) The average particle size, encapsulation efficiency and Zeta potential of the green tea extract nanoliposomes prepared in step (3) were determined.

[0045] After testing, the average particle size of the green tea extract nano-liposome prepared in this example...

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Abstract

A preparation method for a nano lipidosome containing a green tea extractive belongs to the field of chemistry. The nano lipidosome comprises the green tea extractive, soya lecithin and cholesterol; the preparation method comprises the following steps: proportionally dissolving soya lecithin and cholesterol in chloroform to obtain a mixed solution, obtaining a transparent film through reduction vaporization, adding a phosphate buffer salt solution containing the green tea extractive, rotating till the film is dissolved, removing the remaining solvent, and performing ultrasonic processing to obtain a nano lipidosome suspension containing the green tea extractive. According to the invention, encapsulation efficiency and average particle diameter are taken as the indexes, the process parameters and the optimal prescription in preparation of the nano lipidosome containing the green tea extractive are determined through single factor experiments and orthogonal experiments. Compared with a collagen membrane added in a common green tea extractive, oxidation resistance stability, mechanical property, water and light resistance, and hydrophobicity of the collagen film added in the nano lipidosome containing the green tea extractive and prepared through the method are all improved, so that relatively good effects of food protection and environmental pollution reduction can be achieved.

Description

Technical field [0001] The invention involves a preparation method of nano -lipids containing green tea extracts, which belongs to the chemical field. Background technique [0002] With the development of science and technology and the improvement of people's living standards, people's attention to environmental issues is getting higher and higher, and the requirements for the quality, packaging and waste packaging of goods are becoming more and more stringent. In this case"It came into being.Functional food packaging membranes made of natural biological materials have become research hotspots in the field of food packaging.The functional packaging membrane can add some active ingredients to improve the antioxidant performance, antibacterial performance or nutritional value of the membrane.According to data, the oxidation effect is one of the main reasons for food corruption. Adding antioxidants to preparing packaging materials with antioxidant properties are increasingly valued....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23P1/00A61K36/82A61K9/127A61P39/06A61P35/00A61P17/16A61P3/06A61P3/10A61P9/12A61P31/04A23L33/00
CPCA23V2002/00A61K36/00A23V2200/302A23V2200/308A23V2200/328A23V2200/30
Inventor 李建华张德彬王双双苗静伍久林张其清
Owner FUZHOU UNIV
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