Antimicrobial peptide AP-57 as well as preparation method and application method thereof

Abstract

The invention discloses an antimicrobial peptide AP-57 as well as a preparation method and an application method thereof. The antimicrobial peptide AP-57 is a polypeptide and contains 57 amino acids. The preparation method comprises the following steps: optimizing the eukaryotic expression nucleotide sequence of the human AP-57 into the nucleotide sequence suitable for escherichia coli expression; transforming a recombinant expression plasmid to engineered escherichia coli BL21 competent cells; picking up a monoclonal antibody for enlarged cultivation and inducible expression; performing affinity chromatography; performing cation exchange chromatography; finally, performing mass spectrum identification. The antimicrobial peptide AP-57 has the functions of broad-spectrum antiviral microorganisms, is capable of resisting bacteria, fungi, viruses and mycoplasmas, and is a novel human antimicrobial peptide having a plurality of biological functions; the antimicrobial peptide AP-57 has remarkable antibacterial and antifungal effects and a prominent effect in resisting enveloped virus and mycoplasma, and thus has a great potential application value in the aspects of inhibiting the growth, breeding and spreading of microorganisms and preventing or treating pathogenic microorganism infection.

Description

technical field [0001] The invention belongs to the technical field of biochemistry and biomedicine, and in particular relates to an antimicrobial peptide AP-57 and its preparation method and application method. Background technique [0002] Microbial infections, including bacteria, fungi, viruses, etc., can cause many very serious human diseases including hepatitis, pneumonia, AIDS, tuberculosis, etc. Infectious epidemics caused by pathogenic microorganisms are always one of the important causes of human death. [0003] Since the invention of antibiotics, the lives of countless patients with bacterial infections have been saved. However, the use of a large number of antibiotics not only brings many side effects to patients, but also causes the breeding of a large number of drug-resistant strains. And antibiotics are often ineffective against viral infections. [0004] Antimicrobial peptides are a general term for short peptides with antibacterial activity. In 1975, Swed...

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Problems solved by technology

[0018] The purpose of the embodiments of the present invention is to provide an antimicrobial peptide AP-57 and its preparation method and application method, aiming at solving the problem that there are relatively few types of human antimicrobial peptides in the existing technology, and the solid-phase synthesis of peptides is suitable for the number of amino acids. For target polypeptides with fewer and fewer disulfide bonds, production and quality control are more difficult for proteins with more amino acid numbers and more disulfide bonds, and the production cost is relatively high

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