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Method for extracting mesona chinensis benth polysaccharide through microbial fermentation

A microbial fermentation method and the technology of celestial grass polysaccharides, which are applied in the field of microbial fermentation to extract polysaccharides from plants, can solve the problems of low yield and large alcohol consumption, and achieve high yield, reduced emissions, and low-carbon and environmental protection in the extraction process Effect

Active Publication Date: 2015-03-04
SHENZHEN SHENBAO HUACHENG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional alcohol precipitation process consumes a lot of alcohol and the yield is relatively low

Method used

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  • Method for extracting mesona chinensis benth polysaccharide through microbial fermentation
  • Method for extracting mesona chinensis benth polysaccharide through microbial fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1) Pulverization: The dried grass jelly was pulverized to 20 mesh by a multifunctional pulverizer.

[0036] 2) First extraction: put the pulverized grass jelly into a fermenter, add 8 times the volume of boiling pure water of jelly jelly, adjust the pH to 4 with potassium dihydrogen phosphate buffer solution, heat the extract for 40 minutes and cool to 28°C.

[0037] 3) Preparation of fermenting bacteria seed liquid: Aspergillus niger and Aspergillus oryzae were activated at a ratio of 1:2, amplified and cultured on a shaking table at 200 rpm at 25°C for 18 hours, and amplified and cultured to obtain mixed fermented bacteria seeds liquid.

[0038] 4) Inoculation: Inoculate the fermented bacteria seed liquid prepared in step 3) into the fermenter described in step 2) under aseptic conditions with an amount of 5% of the material volume in the fermenter, and stir and mix evenly.

[0039] 5) Fermentation: Seal the fermented material in step 4) and let it stand at 28° C. fo...

Embodiment 2

[0046] 1) Grinding: Grinding the dry grass jelly raw materials to 35 meshes by a multifunctional grinder.

[0047] 2) First extraction: put the pulverized grass jelly into a fermenter, add boiling pure water 6 times the volume of jelly jelly, adjust the pH to 5 with a potassium dihydrogen phosphate buffer solution, heat the extract for 50 minutes, and then cool to 30°C.

[0048] 3) Preparation of fermenting bacteria seed liquid: Aspergillus niger and Aspergillus oryzae were activated at a ratio of 1:1, amplified and cultured on a shaker at 150 rpm at 28°C for 25 hours, and mixed fermented bacteria seeds were obtained from the amplified culture liquid.

[0049] Preparation of medium: Peel potatoes, cut into pieces, add water, heat up to 121°C in a sterilizing kettle, sterilize and boil for 30 minutes, filter with gauze, add agar to the filtrate, boil and dissolve, add glucose, make up water, pack, Sterilize and reserve.

[0050] 4) Inoculation: Inoculate the fermented bacteri...

Embodiment 3

[0058] 1) Grinding: Grinding the dry grass jelly raw material to 50 mesh by a multi-functional grinder;

[0059] 2) First extraction: put the pulverized grass jelly into a fermenter, add 8 times the volume of boiling pure water of jelly jelly, adjust the pH to 6 with a potassium dihydrogen phosphate buffer solution, keep it warm for 60 minutes and then cool to 34°C.

[0060] 3) Preparation of fermenting bacteria seed liquid: Aspergillus niger and Aspergillus oryzae were activated at a ratio of 2:1, amplified and cultivated on a shaker at 200 rpm at 28°C for 20 hours, and amplified and cultivated to obtain mixed fermented bacteria seeds liquid.

[0061] Preparation of medium: Peel potatoes, cut them into pieces, add water, heat up to 121°C in a sterilizing kettle, sterilize and boil for 30 minutes, filter with gauze, add agar to the filtrate, boil to dissolve, add glucose, make up water, pack, Sterilized, spare.

[0062] 4) Inoculation: Inoculate the fermented bacteria seed s...

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Abstract

The invention discloses a method for extracting mesona chinensis benth polysaccharide through microbial fermentation. The method comprises steps of smashing, firstly extracting, preparing of zymophyte seed liquid, inoculating, fermenting, secondary extracting, rough filtering, membrane concentrating, alcohol precipitation and drying. The method integrates biofermentation, membrane concentration and alcohol precipitation technologies, the yield for extracting the mesona chinensis benth polysaccharide is high, the process is environmental friendly, and the production cost is low. The extracting, separating and concentrating processes are mild in conditions, and the extracted mesona chinensis benth polysaccharide is good in taste, high in purity, high in gel strength and good in texture.

Description

technical field [0001] The invention relates to a method for microbial fermentation and extraction of polysaccharides in plants, in particular to a method for microbial fermentation and extraction of jellyfish polysaccharides. Background technique [0002] Jelly Grass, also known as: Immortal Grass, Immortal Jelly, Immortal Grass, is a plant of the Labiatae family, with the lower part of the stem lying on the ground. Grass jelly polysaccharide is a gelatinous polysaccharide contained in grass jelly. Grass jelly polysaccharide is composed of 8 kinds of monosaccharides, mannose, rhamnose, ribose, glucuronic acid, galacturonic acid, glucose, galactose and xylose. [0003] At present, the extraction of jelly grass polysaccharide mainly includes processes such as boiling and alcohol precipitation. For example, patent CN1230356A discloses a kind of jelly jelly polysaccharide and its preparation method. 2 CO 3 1. Add water to boil the juice, filter and discard the residue, decol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 周军陈悦水
Owner SHENZHEN SHENBAO HUACHENG TECH
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