A kind of preparation method of agave hemp SOD modified substance mpeg-sod freeze-dried powder

A technology of agave hemp and freeze-dried powder, applied in biochemical equipment and methods, enzymes, oxidoreductases, etc., can solve the problem of low resistance to proteolysis, reduce or eliminate immunogenicity, and improve activation efficiency , the effect of shortening the activation time

Active Publication Date: 2017-04-26
LINGNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the method is non-toxic and harmless, and has high safety, the thermal stability, acid-base stability, and proteolytic resistance of natural agave hemp superoxide dismutase are relatively low, so it is necessary to improve its thermal stability and acid-base stability. sex matters

Method used

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  • A kind of preparation method of agave hemp SOD modified substance mpeg-sod freeze-dried powder
  • A kind of preparation method of agave hemp SOD modified substance mpeg-sod freeze-dried powder
  • A kind of preparation method of agave hemp SOD modified substance mpeg-sod freeze-dried powder

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Experimental program
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Effect test

Embodiment 1

[0025] A preparation method of agave hemp SOD modified mPEG-SOD freeze-dried powder, using microwave-assisted cyanuric chloride to activate polyethylene glycol monomethyl ether (mPEG) as an activation modifier, and agave hemp superoxide Dismutase (SOD) is the object of modification, and modified agave hemp SOD (mPEG-SOD) is prepared.

[0026] 1. The mPEG modifier consists of the following parts by mass:

[0027] 50 parts by mass of mPEG, 10 parts by mass of 4A molecular sieve, 20 parts by mass of anhydrous sodium carbonate and 2.5 parts by mass of cyanuric chloride, 300 mL of acetone.

[0028] 2. The mPEG modifier is prepared by the following steps:

[0029] Transfer the various components into a microwave oven with a reflux device, select a power of 40%, and react for 35 minutes. Suction filtration while the reaction is hot, pour the supernatant into 500 mL of anhydrous diethyl ether or petroleum ether, cool and precipitate in an ice bath, and suction filtration to obtain p...

Embodiment 2

[0034] A preparation method of agave hemp SOD modified mPEG-SOD freeze-dried powder, using microwave-assisted cyanuric chloride to activate polyethylene glycol monomethyl ether (mPEG) as an activation modifier, and agave hemp superoxide Dismutase (SOD) is the object of modification, and modified agave hemp SOD (mPEG-SOD) is prepared.

[0035]1. The mPEG modifier consists of the following parts by mass:

[0036] 50 parts by mass of mPEG, 10 parts by mass of 4A molecular sieves, 15 parts by mass of anhydrous sodium carbonate and 3.5 parts by mass of cyanuric chloride, 300 mL of N,N-dimethylformamide.

[0037] 2. The mPEG modifier is prepared by the following steps:

[0038] Transfer the various components into a microwave oven with a reflux device, select a power of 70%, and react for 25 minutes. Suction filtration while the reaction is hot, pour the supernatant into 500 mL of petroleum ether, cool and precipitate in an ice bath, and suction filtration to obtain the precipitat...

Embodiment 3

[0043] A preparation method of agave hemp SOD modified mPEG-SOD freeze-dried powder, using microwave-assisted cyanuric chloride to activate polyethylene glycol monomethyl ether (mPEG) as an activation modifier, and agave hemp superoxide Dismutase (SOD) is the object of modification, and modified agave hemp SOD (mPEG-SOD) is prepared.

[0044] 1. The mPEG modifier consists of the following parts by mass:

[0045] 50 parts by mass of mPEG, 10 parts by mass of 4A molecular sieve, 15 parts by mass of anhydrous sodium carbonate and 1 part by mass of cyanuric chloride, 300 mL of anhydrous toluene.

[0046] 2. The mPEG modifier is prepared by the following steps:

[0047] Transfer the various components into a microwave oven with a reflux device, select a power of 40%, and react for 25 minutes. Suction filtration while the reaction is hot, pour the supernatant into 500 mL of anhydrous diethyl ether, cool and precipitate in an ice bath, and suction filtration to obtain the precipita...

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Abstract

The invention relates to a preparation method of lyophilized agave SOD modifier mPEG-SOD powder, and belongs to the technical field of organic synthesis and application. The method concretely comprises the following steps: an activation modification agent is obtained through microwave radiation activation on polyethylene glycol monomethyl ether by cyanuryl chloride with anhydrous N,N-dimethyl formamide (DMF), toluene or acetone as a solvent, and the agave superoxide dismutase modifier is prepared with an agave superoxide dismutase as a modification object. The preparation method of the activation modifier substantially shortens the activation time and improves the activation efficiency by means of microwave radiation heating; and the modifier obtained through modifying the agave superoxide dismutase by the activation modification agent has the better thermal stability, soda acid stability and proteolysis resistance than natural agave superoxide dismutase, and has decreased or eliminated immunogenicity.

Description

technical field [0001] The present invention relates to a preparation method of agave hemp SOD modification mPEG-SOD freeze-dried powder, specifically using anhydrous N,N-dimethylformamide DMF, toluene or acetone as a solvent, and irradiating cyanuric chloride with microwaves The invention relates to an activation modifier obtained by activating polyethylene glycol monomethyl ether, using agave hemp superoxide dismutase as a modification object to prepare agave hemp superoxide dismutase modified product, which belongs to the technical field of organic synthesis and application. Background technique [0002] Superoxide dismutase (superoxide dismutase SOD) is a metalloprotease that is widely present in living organisms and can specifically scavenge superoxide anion free radicals. [0003] Organisms produce reactive oxygen species with oxygen metabolism during normal life activities. Organisms have a very complete active oxygen scavenging system, including various antioxidant ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/96C12N9/02
CPCC12N9/0089C12N9/96C12Y115/01001
Inventor 陈冰
Owner LINGNAN NORMAL UNIV
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