A kind of curcumin nanoparticle and preparation method thereof
A technology of curcumin and nanoparticles, applied in the field of curcumin nanoparticles and preparation thereof, to achieve the effects of reducing speed and degree, prolonging circulation time and improving bioavailability
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[0031] The preparation of polymer refers to literature (Lu, D.D., et al.Journal of Polymer Science Part A: Polymer Chemistry, 2008, 46 (11), 3802-3812; Lee, H., etal. Biomacromolecules, 2005, 6 (6 ), 3119-3128; Oh, J.M., et al. Polymer, 2009, 50(25), 6019-6023), the specific operation is as follows:
[0032] Monomethoxypolyethylene glycol-2000 (20g, 10mmol), metal sodium (2.3g, 100mmol) were dissolved in tetrahydrofuran (60mL), and reacted at 60°C for 10h under nitrogen atmosphere. Add epichlorohydrin (5.55 g, 60 mmol), react at the same temperature for 10 h, cool to room temperature, filter, and vacuum rotary evaporate to obtain monomethoxypolyethylene glycol glycidyl ether.
[0033] The monomethoxypolyethylene glycol glycidyl ether obtained above was mixed with 10% sodium hydroxide (140 mL, 389 mmol), and reacted at 80° C. for 10 h. Evaporate in vacuo to dryness, add acetonitrile (100 mL) to dissolve the residue, filter, and evaporate the organic solvent in vacuo to obtain ...
Embodiment 1
[0039] Polymer Line-Tree MPEG 2000 -PCL4 1000 (50mg) and curcumin (10mg) were dissolved in 2mL of methanol, ultrasonically assisted, and rotary evaporated at room temperature to make an adherent film. Add 5mL of preheated water for injection at 50°C and slowly rotate for hydration. Microporous membrane The unencapsulated curcumin was removed by filtration to prepare a nanoparticle solution with an encapsulation efficiency of 62.3% and a drug loading capacity of 12.46%. The obtained nanoparticle solution is freeze-dried to obtain a powdery drug-loaded nanoparticle solid.
Embodiment 2
[0041] Polymer Line-Tree MPEG 2000 -PCL4 4000 (50mg) and curcumin (10mg) were dissolved in 2mL of ethanol, ultrasonically assisted, and made into a wall-adhering film by rotary evaporation at room temperature, and 5mL of preheated water for injection was added at 65°C to slowly hydrate by rotary rotation, and the microporous membrane The unencapsulated curcumin was removed by filtration to prepare a nanoparticle solution with an encapsulation efficiency of 84.5% and a drug loading capacity of 16.9%. The obtained nanoparticle solution is freeze-dried to obtain a powdery drug-loaded nanoparticle solid.
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