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Organic and inorganic hybrid microsphere particle, preparation and application thereof

A technology for hybridizing microspheres and particles, applied in the field of enrichment of glycoproteins, can solve the problem of no improvement in the hydrophilicity of materials, achieve good practical value and application prospects, high reaction efficiency, and increase the effect of bonding amount.

Active Publication Date: 2015-03-18
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

X Zhang, X He, L Chen, Y Zhang, J. Mater. Chem., 2012, 22, 16520 reported a magnetic Fe 3 o 4 A preparation method of phenylboronic acid modified on the surface of microspheres, which immobilizes phenylboronic acid containing azide groups to magnetic Fe by "click chemistry" 3 o 4 The surface of microspheres effectively improves the immobilization rate of phenylboronic acid and significantly improves its ability to enrich glycoproteins, but this technology belongs to the metal-catalyzed reaction of azide and alkyne, and the preparation of this material does not make The hydrophilicity of the material is also improved

Method used

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  • Organic and inorganic hybrid microsphere particle, preparation and application thereof
  • Organic and inorganic hybrid microsphere particle, preparation and application thereof

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Embodiment 1

[0020] 1. Preparation of monodisperse core-shell polymer nanoparticles

[0021] In a 150mL round bottom flask, add 80mL of toluene, add 6g of silica gel particles with a particle size of 5μm, and 3g of 3-mercaptopropyltriethoxysilane, and sonicate for 1 minute, so that the added reagents and particles are evenly dispersed in the solution In the process, a condenser was connected to the flask, a magnetic stirrer was added, and the magnetic stirrer was kept at a speed of 300 rad / min. The reaction device was placed in an oil bath, heated to reflux for 6h, then the reaction was stopped and cooled to room temperature. Then use a high-speed centrifuge to centrifuge at a speed of 10,000 rad / min, remove the supernatant, use toluene, acetone, methanol, and acetone to filter and wash in sequence, repeat the suction and filter washing three times, and vacuum dry in a vacuum oven at 50°C for 24 hours. Hours, the modified silica gel particles of the silylating agent were obtained.

[002...

Embodiment 2

[0031] 1. Preparation of monodisperse core-shell polymer nanoparticles

[0032] In a 150mL round bottom flask, add 80mL of toluene, add 5g of silica gel particles with a particle size of 5μm, 3g of 3-mercaptopropyltriethoxysilane, and sonicate for 1 minute, so that the added reagents and particles are evenly dispersed in the solution In the process, a condenser was connected to the flask, a magnetic stirrer was added, and the magnetic stirrer was kept at a speed of 300 rad / min. The reaction device was placed in an oil bath, heated to reflux for 6h, then the reaction was stopped and cooled to room temperature. Then use a high-speed centrifuge to centrifuge at a speed of 10,000 rad / min, remove the supernatant, use toluene, acetone, methanol, and acetone to filter and wash in sequence, repeat the filter and wash 3 times, and vacuum dry in a vacuum oven at 50°C for 24 hours to obtain Silica gel particles modified with silylating agents. In a 100mL round bottom flask, install a c...

Embodiment 3

[0035] 1. Preparation of monodisperse core-shell polymer nanoparticles

[0036] In a 150mL round bottom flask, add 80mL of toluene, add 4g of silica gel particles with a particle size of 5μm, and 3g of 3-mercaptopropyltriethoxysilane, and sonicate for 1 minute, so that the added reagents and particles are evenly dispersed in the solution In the process, a condenser was connected to the flask, a magnetic stirrer was added, and the magnetic stirrer was kept at a speed of 300 rad / min. The reaction device was placed in an oil bath, heated to reflux for 6h, then the reaction was stopped and cooled to room temperature. Then use a high-speed centrifuge to centrifuge at a speed of 10,000 rad / min, remove the supernatant, and use toluene, acetone, methanol, and acetone to filter and wash in sequence, repeat the filter and wash three times, and dry in a vacuum oven at 50°C. After 24 hours, silica gel particles of the modified silylating agent were obtained. In a 100mL round bottom flas...

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Abstract

The invention belongs to the technical fields of polymer materials and analysis, relates to a monodispersed organic and inorganic hybrid microsphere particle with core-shell structure and preparation and application thereof. Inorganic silica particles as the core are modified by a silanizing agent, and then 3-acetylaminophenylboronic acid and polyene cross-linking monomer coat on the click nuclear surface through a mercaptan-alkene click chemistry technology to form organic and inorganic hybrid nano / micron particles with core-shell structure, smooth surface and boric acid functional groups. According o the invention, introduction of the phenyl boronic acid functional group to the material surface by a mercaptan-alkene method not only overcomes the shortcomings of complex procedure and low reaction efficiency in a post modification method but also allows stable polymerization of APBA, increases surface bonding amount of boric acid and hydrophilic property of the material, and reduces the nonspecific adsorption of proteins. The microsphere particles of the invention can be used in separation or enrichment of glycoprotein with 1,2-cis diol structure, and has good practical value and application prospect in the field of proteomics.

Description

technical field [0001] The invention relates to enriching glycoproteins, in particular to a monodisperse organic-inorganic hybrid core-shell structure microsphere particle and its preparation and application in glycoprotein enrichment. Background technique [0002] As we all know, the composition of proteins in organisms is extremely complex and constantly changing. Even in different cells of the same species or in different stages of the same cell, there are significant differences in the composition of proteins. Therefore, proteomics has gradually become the focus of attention. In proteomics, glycoproteins, as an important class of post-translational modification proteins, play an important role in biological processes such as signal transduction, immunity and tumorigenesis. Therefore, the discovery and identification of glycoproteins and glycopeptides is of great significance in the field of disease diagnosis and proteomics. At present, the main methods for the enrichmen...

Claims

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Application Information

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IPC IPC(8): C08F292/00C08F230/06G01N1/40
Inventor 张丽华刘键熙杨开广吴琪曲焱焱李森武
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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