A kind of artificial cornea with nano-ultra-thin biofilm differentially modifying the front and rear surfaces and its production method

Abstract

The invention relates to a keratoprosthesis with front and back surfaces differently ornamented by a nanometer ultrathin biological membrane and a preparation method. The invention aims to provide the keratoprosthesis for reducing keratoprosthesis postoperative complications. The provided preparation method is simple, practicable and pollution-free. According to the keratoprosthesis with front and back surfaces differently ornamented by the nanometer ultrathin biological membrane, polycation layers and epidermal growth factor layers are repeatedly deposited on the front surface of an optical part sequentially, the polycation layers and HA layers are repeatedly deposited on the rear surface of the optical part from inside to outside sequentially, and the outmost layer is the HA layer. The preparation method of the keratoprosthesis with front and back surfaces differently ornamented by the nanometer ultrathin biological membrane sequentially comprises the following steps: (1) charging negative charge to the surface of the keratoprosthesis; (2) alternately absorbing polycations and epidermal growth factors on the front surface of the keratoprosthesis; (3) alternately absorbing the polycations and HA on the rear surface of the keratoprosthesis; and (4) drying and carrying out seal packing.

Description

technical field [0001] The invention relates to the medical field, especially an artificial cornea and a manufacturing method thereof. Background technique [0002] Corneal disease is the second leading cause of blindness in the world after cataract. Of the 45 million blind people in the world, 8 million are blinded by corneal diseases, and an average of 1.5-2 million new cases of blindness due to corneal ulcers and trauma are added each year. Corneal transplantation is currently the main treatment for severe corneal diseases. Although there are more than 1 million blind patients due to corneal diseases in my country, there are less than 5,000 cases of corneal transplantation each year. The lack of corneal donors has always been and is increasingly becoming an important constraint for corneal transplantation. Factors; certain severe corneal diseases such as chemical burns, stem cell defects, ocular scar pemphigoid, Stevens-Johnson syndrome, etc., corneal transplantation cann...

AI Technical Summary

Problems solved by technology

Corneal transplantation is currently the main treatment for severe corneal diseases. Although there are more than 1 million blind patients due to corneal diseases in my country, less than 5,000 cases of corneal transplantation are performed each year. The lack of corneal donors has always been and is increasingly becoming an important constraint for corneal transplantation. Factors; certain severe corneal diseases such as chemical burns, stem cell defects, ocular scar pemphigoid, Stevens-Johnson syndrome, etc., corneal transplantation cannot be successful

Both PMMA and pHEMA have irreversible shortcomings related to the properties of the body. The former is a hard hydrophobic material, which cannot measure intraocular pressure after implantation, is easily damaged by Nd:YAG laser, and has poor biocompatibility. The latter has higher requirements on the ocular surface. , poor mechanical strength, prone to calcification and deposition in the optical part, etc., resulting in a high incidence of artificial corneal opacity

The designs of both are relatively complicated. The Boston artificial cornea is a "mirror column-stent" structure, which is composed of three parts. The strength of the junction is poor, and it is easy to leak aqueous humor. Alphacor is composed of a transparent optical part and an opaque peripheral part. , although both parts are pHEMA components, they cannot be integrally formed

Both of them also have the problems of poor long-term postoperative effect and high risk of complications due to the failure of the front surface to support the growth of corneal epithelial cells and the formation of an artificial corneal posterior membrane on the back surface as mentioned above.

In addition, both have the problems of large corneal incision (Boston’s incision is about 25mm, and Alphacor’s about 16mm), and the operation time is long (more than 1 hour). However, the implantation of Boston still needs the support of the donor cornea, and Alphacor needs two surgeries. Surgery to complete artificial cornea implantation

[0005] Chinese invention patent 201110218237.3 "An artificial cornea" has a surface modification layer of fluorine-heparin on the inner and outer surfaces of the optical zone through covalent bonding or electrostatic adsorption, which is beneficial to prevent pathological cell and tissue adsorption; but this patent does not take artificial The difference in the environment of the front and back surfaces of the cornea and the difference in their requirements for biocompatibility, both the front and back surfaces are modified with fluorine-heparin that inhibits adhesion, and there is still the problem of difficulty in the epithelialization of the front surface

Chinese invention patent 02116963.2 "Preparation method of polyvinyl alcohol tool body hydrogel soft artificial cornea" also proposes to use energy-carrying ion beam combined with low-temperature plasma surface coating technology to coat the inner surface of the non-peripheral part of the main body of the artificial cornea with low Molecular heparin; however, the patent only proposes coating the inner surface with low molecular weight heparin, without considering the need for epithelialization of the anterior surface

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