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Method for realizing chemosensitivity influence on laryngeal carcinoma cell lines by iron-carbon nanoparticle mediated cisplatin

A nanoparticle and cancer cell technology, applied in the field of clinical medicine, can solve laryngeal cancer radiotherapy and chemotherapy sensitivity, poor quality of life, dyspnea and other problems

Inactive Publication Date: 2015-03-25
朱力
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Problems solved by technology

[0005] Laryngeal cancer is a common malignant tumor in clinical practice. Surgery is the main method of comprehensive treatment in clinical practice. Long-term wearing of tubes or tracheostomy due to stenosis seriously affects the quality of life of patients, and the postoperative recurrence rate is high; radiotherapy and chemotherapy do not destroy the anatomical structure of the larynx, and some complications can be avoided
However, laryngeal cancer is less sensitive to radiotherapy and chemotherapy

Method used

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  • Method for realizing chemosensitivity influence on laryngeal carcinoma cell lines by iron-carbon nanoparticle mediated cisplatin
  • Method for realizing chemosensitivity influence on laryngeal carcinoma cell lines by iron-carbon nanoparticle mediated cisplatin
  • Method for realizing chemosensitivity influence on laryngeal carcinoma cell lines by iron-carbon nanoparticle mediated cisplatin

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preparation example Construction

[0094] Preparation of standard curve:

[0095] The standard was diluted sequentially with deionized water to 4×10 2 , 4×10 3 , 4×104, 4×10 5 , 4×10 6 ; Standard curves were prepared for each amplification with a range of 10 per mL 2 -10 6 ;copy;

[0096] Calibration and judgment analysis of PCR product quantification:

[0097] GAPDH was used as the internal reference, and Survivin mRNA and GAPDH mRNA were used to obtain the molecular copy number of mRNA according to the standard curve; the copy number of GAPDH was used as the calibration base, that is, the accurate content of target gene mRNA = target gene CT value / internal reference GAPDH CT value (Survivin and GAPDH ratio as the expression level of the Survivin gene); this ratio was used for statistical processing; according to the principle of FQ-PCR, the excited fluorescent signal was collected by the fluorescent probe after reaching a certain threshold, and finally converted into a CT value. The actual copy number ...

specific Embodiment

[0101] The first step, the preparation of iron carbon nanoparticles:

[0102] Use electronic balance to weigh ferric oxide and graphite powder; use high-energy ball milling technology to prepare iron-carbon nanoparticles, wash the ball milling tank with distilled water after the ball milling, magnetize the iron-containing particles, and vacuum-dry the obtained washing liquid for later use to obtain dry Iron carbon nanoparticles;

[0103] In the second step, iron-carbon nanoparticles are loaded with cisplatin:

[0104] Magnetic iron-carbon nanoparticle cisplatin was prepared by ultrasonic emulsification-solvent evaporation method; iron-carbon nanoparticle and cisplatin were dissolved in dichloromethane, and the suspension obtained after ultrasonic emulsification was placed on a magnet, washed, centrifuged, and the obtained The precipitate is freeze-dried, vacuum-dried, and irradiated to sterilize to obtain magnetic iron-carbon nanoparticle cisplatin;

[0105] The third step i...

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Abstract

The invention discloses a method for realizing chemosensitivity influence on laryngeal carcinoma cell lines by iron-carbon nanoparticle mediated cisplatin. The method comprises preparation of iron-carbon nanoparticles, loading of cisplatin by the iron-carbon nanoparticles as well as influence of iron-carbon nanoparticle mediated cisplatin on laryngeal carcinoma Hep-2 cell microstructure, proliferation activity as well as Survivin mRNA (messenger ribonucleic acid) and caspase3 mRNA expression. According to the invention, the magnetic nano material is adopted for the first time to load cisplatin, a chemotherapeutic drug with higher magnetism and targeting is provided, the preoperative chemotherapeutic effect on laryngeal carcinoma can be improved after the drug is applied to clinical practice, the necessary and efficient chemotherapeutic drug is provided for functional laryngeal carcinoma radical treatment is provided, and the drug can be applied to postoperative chemotherapy simultaneously; and toxic and side effects of the chemotherapeutic drug are reduced, the iron-carbon nano material has high drug loading capacity, stable release rate and higher targeting, and the iron-carbon nanoparticle mediated cisplatin can play an important role in clinical popularization and application for laryngeal carcinoma treatment.

Description

technical field [0001] The invention belongs to the technical field of clinical medicine, and in particular relates to a method for mediating the influence of cisplatin on the chemosensitivity of laryngeal cancer cell lines by iron-carbon nanoparticles. Background technique [0002] Laryngeal cancer is one of the common malignant tumors in ENT surgery. Although significant progress has been made in the treatment of malignant tumors in recent years, the postoperative recurrence of patients with laryngeal cancer and the decline in postoperative laryngeal function are still the bottlenecks in the treatment of laryngeal cancer. Surgery-based comprehensive treatment is the main treatment mode for laryngeal cancer. In recent years, with the introduction of the concept of functional laryngeal cancer surgery, the scope of surgical resection of laryngeal cancer tends to be reduced. Preoperative and postoperative radiotherapy and chemotherapy play an increasingly prominent role in red...

Claims

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Application Information

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IPC IPC(8): C12Q1/02C12Q1/68
Inventor 朱力
Owner 朱力
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