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Preparation method of brain protein hydrolysate

A technology of brain protein hydrolyzate and neutral protease, which is applied in the field of medicine, can solve problems such as protein denaturation and complicated procedures, and achieve the effects of high activity, simple preparation method, and strong pharmacological activity

Active Publication Date: 2015-04-15
西藏易明西雅医药科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] CN200410022091.5 discloses a preparation method of cerebroprotein hydrolyzate. The invention prepares cerebroprotein hydrolyzate by successively using pepsin and trypsin for enzymolysis. problems with protein denaturation

Method used

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  • Preparation method of brain protein hydrolysate
  • Preparation method of brain protein hydrolysate
  • Preparation method of brain protein hydrolysate

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0024] Experimental Example 1 Animal Pharmacodynamics Test

[0025] Test drug: commercially available compound cerebroprotein hydrolyzate tablets (purchased from Dalian Beier Pharmaceutical Co., Ltd.), the sample prepared in Example 5 of the present invention, and distilled water to make a solution of required concentration for oral administration.

[0026] 1) Effects on the hypobaric hypoxic state of mice

[0027] Take 150 mice of 18-21g, half male and half male, and randomly divide them into 3 groups, and administer them by intragastric administration according to the drug dosage shown in Table 1. After 30 minutes, put the three groups into the vacuum chamber at the same time, airtight, and decompress Vacuum was applied, and when more than half of the mice in the control group died, observation was continued for 30 minutes after ventilation, and the number of dead animals in each group was recorded.

[0028] The results are shown in Table 1.

[0029] Table 1 Experimental s...

experiment example 2

[0040] Experimental Example 2 Determination of peptide and total nitrogen content in cerebroprotein hydrolyzate

[0041] 1. Experimental drug

[0042] Take the samples prepared according to the methods of Example 3, Example 5, Example 8 and CN200410022091.5.

[0043] 2. The method for measuring the cerebroprotein hydrolyzate content (in amino nitrogen) in the cerebroprotein hydrolyzate

[0044] Take an appropriate amount of sample (approximately equivalent to 50 mg of peptide), accurately weigh it, put it in a digestive tube, add an appropriate amount of hydrochloric acid (so that the test sample is completely submerged and does not exceed 2 / 3 of the volume of the container), fill the seal with nitrogen, and hydrolyze at 110°C for 20 hours, let cool, unsealed, transfer the whole amount of the hydrolyzate to an evaporating dish, evaporate to dryness in a water bath, add water to dissolve the residue and dilute to an appropriate concentration, as the test solution for the deter...

Embodiment 1

[0065] a) Raw material processing: Remove the capsule and blood vessels on the surface of the fresh pig brain, wash it, drain it, and set it aside;

[0066] b) Pre-treatment: mince the pig brain obtained in step a), add it to 2 times the weight of water, homogenize it at room temperature, and freeze it for storage;

[0067] c) Compound enzyme hydrolysis: add the pig brain plasma obtained in step b) to a compound enzyme with a weight ratio of 1:2, and hydrolyze it at a temperature of 40°C. The weight percentage of the compound enzyme is 0.5% of the pig brain pulp, and hydrolyze for 8 hours. After the hydrolysis is completed, put it in boiling water Keep the enzyme in the medium for 8 minutes, adjust the pH to 6 after cooling, filter, and finally filter the hydrolyzate with an ultrafiltration membrane to remove the hydrolyzate with a molecular weight greater than 10kDa, collect the ultrafiltration hydrolyzate and store it at 4°C;

[0068] d) Concentration: the ultrafiltration hy...

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Abstract

The invention belongs to the technical field of medicine, and provides a preparation method of brain protein hydrolysate. The preparation method comprises steps of raw material treatment, pre-treatment, composite enzyme hydrolysis, pH value adjustment, ultrafiltration, and nano filtration. The preparation method is simple and can be easily applied to industrial production. A certain amount of composite enzyme is used to carry out hydrolysis, and the obtained brain protein hydrolysate has the advantages of high peptide content and high vitality.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method of cerebroprotein hydrolyzate. Background technique [0002] Cerebroprotein hydrolyzate drugs are one of the most widely used biochemical drugs in recent years. It occupies an important position in related research fields and markets at home and abroad. Cerebroprotein hydrolyzate, as an active brain polypeptide substance extracted from healthy and fresh animal brain tissue by enzymolysis, contains a variety of essential amino acids for human brain and important elements such as cephalin, lecithin, and peptide nerve growth factor. This way acts on the central nervous system, regulates and improves the metabolism of neurons, promotes the formation of synapses, induces the differentiation of neurons, and further protects nerve cells from various ischemia and neurotoxin damage. It can pass through the blood-brain barrier, promote the synthesis of p...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K35/30A61P3/02A61P39/00A61P25/00
Inventor 曹捷高帆周战尚磊
Owner 西藏易明西雅医药科技股份有限公司
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