Preparation method of brain protein hydrolysate
A technology of brain protein hydrolyzate and neutral protease, which is applied in the field of medicine, can solve problems such as protein denaturation and complicated procedures, and achieve the effects of high activity, simple preparation method, and strong pharmacological activity
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[0024] Experimental Example 1 Animal Pharmacodynamic Test
[0025] Test drug: commercially available compound brain protein hydrolysate tablets (purchased from Dalian Bell Pharmaceutical Co., Ltd.), the sample prepared in Example 5 of the present invention, were prepared with distilled water to form a solution of the required concentration for oral administration.
[0026] 1) Effect on hypobaric hypoxia in mice
[0027] Take 150 18-21g mice, half male and half male, and randomly divide them into 3 groups, and administer them by gavage according to the drug dosage shown in Table 1. After 30 minutes, put the three groups into the vacuum chamber at the same time, airtight and decompress. Vacuum, when more than half of the mice in the control group died, continue to observe for 30 minutes after ventilation, and record the deaths of each group of animals.
[0028] The results are shown in Table 1.
[0029] Table 1 Experimental study of mice hypoxia tolerance
[0030]
[0031]
[0032] It can...
Example Embodiment
[0040] Experimental example 2 Determination of peptide and total nitrogen content in brain protein hydrolysate
[0041] 1. Test drugs
[0042] Take samples prepared according to the methods of Example 3, Example 5, Example 8 and CN200410022091.5.
[0043] 2. Method to determine the content of brain protein hydrolysate (calculated as amino nitrogen) in brain protein hydrolysate
[0044] Take an appropriate amount of the sample (approximately equivalent to 50 mg of peptide), accurately weigh it, place it in the digestion tube, add an appropriate amount of hydrochloric acid (so that the test product is completely immersed and does not exceed 2 / 3 of the container's volume), filled with nitrogen, sealed, and hydrolyzed at 110°C for 20 After hours, let cool, unsealed, transfer the entire amount of the hydrolysate to an evaporating dish, evaporate to dryness in a water bath, add water to dissolve the residue and dilute to an appropriate concentration, as the test solution for total amino aci...
Example Embodiment
[0064] Example 1
[0065] a) Raw material processing: remove the surface capsule and blood vessels from the fresh pig brain, wash it, drain the water, and set aside;
[0066] b) Pre-treatment: chop the pig brain obtained in step a) and add it to 2 times the weight of water, homogenize it at room temperature, and store it in freezing;
[0067] c) Compound enzyme hydrolysis: add the pig brain pulp obtained in step b) to the compound enzyme with a weight ratio of 1:2, and hydrolyze at a temperature of 40°C. The weight percentage of the compound enzyme is 0.5% of the pig brain pulp. The hydrolysis is 8 hours, and the hydrolysis is completed in boiling water Keep it for 8 minutes to inactivate the enzyme, adjust the pH to 6 after cooling, filter, and finally filter the hydrolysate with an ultrafiltration membrane to remove hydrolysates with a molecular weight greater than 10kDa, and collect the ultrafiltration hydrolysate for storage at 4°C;
[0068] d) Concentration: the ultrafiltration h...
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