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Method for extracting refined egg yolk antibody by utilizing chick infectious bursal disease virus

A technology of chicken infectivity and yolk antibody, which is applied in the field of veterinary biological products, can solve the problems of product residual toxicity and complicated process, and achieve the effect of compact structure, high purity and short production cycle

Inactive Publication Date: 2015-04-22
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the technical defects of the prior art, and provides a method for extracting chicken infectious bursa refined egg yolk antibody, so as to solve technical problems such as complex process and residual toxicity of the product existing in the extraction method of the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Disinfect 100 kg of fresh chicken bursa hyperimmune eggs and send them to the egg yolk and egg white separator for egg yolk separation, and about 15L egg yolk can be obtained;

[0025] 2) Filter the egg yolk once with a 60-mesh nylon sieve, and grind it once with a colloid mill;

[0026] 3) Pour the ground egg yolk liquid into a 100L material tank, add 37.5L acetic acid buffer solution, turn on the stirring paddle, and stir for 30 minutes;

[0027] 4) Slowly add 2.1L n-octanoic acid, and stir for another 90 minutes;

[0028] 5) Filter the egg yolk liquid treated with octanoic acid with a polypropylene 750B filter cloth overnight;

[0029] 6) Collect the clarified filtrate, prepare the medicine according to the requirements of the chicken infectious bursa refined egg yolk antibody quality standard, and adjust the pH value to 7.0.

Embodiment 2

[0031] A method for extracting chicken infectious bursa yolk antibody, comprising the following steps:

[0032] 1) Get chicken eggs with high immunity to infectious bursal disease, and separate yolk;

[0033] 2) Take the yolk obtained in step 1) and filter to remove the yolk membrane and frenulum, and then grind and break;

[0034] 3) Add acetic acid buffer at a temperature of 50°C and a pH of 4.8 to the egg yolk broken in step 2) at a ratio of 1:2 (w / w), and stir for 40 minutes to obtain a mixture;

[0035] 4) Add n-octanoic acid with a volume of 3% (v / v) of the mixed solution to the mixed solution obtained in step 3), and stir for 30 minutes;

[0036] 5) Filtrate the stirred product in step 4), and collect the filtrate to obtain chicken infectious bursa yolk antibody.

[0037] On the basis of the above technical solutions:

[0038] Step 3) The ratio of sodium acetate, acetic acid and water in the acetic acid buffer solution is: 0.886g:2.82mL:1000mL.

[0039] Step 2) the ...

Embodiment 3

[0043] A method for extracting chicken infectious bursa yolk antibody, comprising the following steps:

[0044] 1) Get chicken eggs with high immunity to infectious bursal disease, and separate yolk;

[0045] 2) Take the yolk obtained in step 1) and filter to remove the yolk membrane and frenulum, and then grind and break;

[0046] 3) Add acetic acid buffer solution with a temperature of 53°C and a pH of 5.0 to the egg yolk broken in step 2) at a ratio of 1:2.5 (w / w), and stir for 60 minutes to obtain a mixed solution;

[0047] 4) Add n-octanoic acid with a volume of 4% (v / v) of the mixed solution to the mixed solution obtained in step 3), and stir for 90 minutes;

[0048] 5) Filtrate the stirred product in step 4), and collect the filtrate to obtain chicken infectious bursa yolk antibody.

[0049] On the basis of the above technical solutions:

[0050] Step 3) The ratio of sodium acetate, acetic acid and water in the acetic acid buffer solution is: 0.886g:2.82mL:1000mL.

...

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PUM

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Abstract

The invention provides a method for extracting a refined egg yolk antibody by utilizing chick infectious bursal disease virus. According to the technical scheme, low ion strength and specific acid condition of octanoic acid are maintained, so that octanoic acid can react with most of egg yolk protein and irreversible precipitate is formed; meanwhile, only IgY antibody protein is unreacted and is still left in liquid, so that egg yolk liquid is diluted and then is subjected to treatment by virtue of the octanoic acid, then solid-liquid separation is carried out on the egg yolk liquid treated by the octanoic acid, IgY aqueous solution with the purity being more than 80% can be obtained, the method for extracting the refined egg yolk antibody is economic, simple and rapid, antibody activity is not damaged, and recovery rate is high.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to a method for extracting chicken infectious bursa refined egg yolk antibody. Background technique [0002] Yolk antibody (immunoglobulin yolk, IgY), that is, birds (multi-fingered chickens) after immunization with specific antigens, produce corresponding specific antibodies, which are transported and stored in egg yolk, and then high levels can be obtained from egg yolk through specific extraction techniques. Yield specific polyclonal antibodies. Due to its stable chemical properties, high yield, low cost, meeting the needs of animal welfare, and the advantages of animal germline distance, IgY is more suitable for the production of specific antibodies, and its passive immune function can be used to study how to resist viruses and bacteria It has the potential to develop functional food and new drugs, so it has a good application prospect in the field of med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C07K16/02C07K1/34
Inventor 杨燚王甜苏建东杨保收梁武
Owner TIANJIN RINGPU BIO TECH
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