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Rapid marsdenia tenacissima propagation culture media

A kind of propagation medium and technology of Tongguanteng, which is applied in the field of rapid propagation of Chinese medicinal material Tongguanteng, can solve the problems of low reproductive rate of Tongguanteng seeds, susceptibility of cutting propagation seedlings, long growth cycle, etc., to achieve suitable scale production, Alleviate the pressure of market drug use and the effect of high rooting rate

Active Publication Date: 2015-04-29
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problems of low seed reproduction rate, long growth cycle, large variability, and easy susceptibility of cutting propagation seedlings of vines, and provide a group of vines that are suitable for large-scale production, fast propagation, and high survival rate. Rapid Propagation Medium Formula

Method used

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  • Rapid marsdenia tenacissima propagation culture media
  • Rapid marsdenia tenacissima propagation culture media

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Selection and treatment of explants

[0018] The explants are derived from the Tongguan vine plant in the wild in Mengzi County, Yunnan Province. The new shoots of the year that are free from diseases and insect pests are selected, and cut into single-bud stem segments with one bud as explants, and are initially sterilized in the following order: Rinse with running water for 2.5 hours, soak in water with a mass fraction of 5% washing powder for 5 minutes, and rinse under running water for 25 minutes; soak the customs-clearing cane section that has undergone preliminary sterilization treatment with a volume fraction of 75% ethanol solution for 1 minute, and then use mass fraction After immersing in 0.1% mercuric chloride solution for 12 minutes, wash with sterile water for 3 to 5 times, and use sterile paper to dry the water on the surface of the cane section for later use.

[0019] Because the twigs of Tongguan vine are densely covered with light yellow fluff, a lot...

Embodiment 2

[0034] Embodiment 2 Except that the following steps are different, the operation of other steps is the same as that of Embodiment 1, and will not be repeated here.

[0035] (1) Selection and sterilization of explants

[0036] The explants came from the wild vine plants of Mengzi County, Yunnan Province, and were transplanted to the new shoots that grew in the same year after domestication in the greenhouse of Yunnan Agricultural University. Other disinfection treatment steps are consistent with the explant treatment method in Example 1, the difference is that the disinfection time is 11 minutes with a mass fraction of 0.1% mercuric chloride solution, which can achieve the lowest pollution rate and high survival rate.

Embodiment 3

[0038] Embodiment 3 is the same as that of Embodiment 1 except that the following steps are different, and will not be repeated here.

[0039] (1) Selection and sterilization of explants

[0040]The explants are derived from the Tongguan vine plants in the wild in Maguan County, Yunnan Province. The young shoots of the year that are free from diseases and insect pests are selected and cut into single-bud stems with one bud as explants. The mass fraction is 0.1% mercuric chloride When the disinfection time was 12 minutes, the pollution rate reached the lowest and the survival rate was high. Its disinfection time is consistent with that of the explants in Example 1 from Mengzi County, Yunnan Province, which is related to the fact that they all come from the wild and have a lot of dust and germs on the plants.

[0041] See Table 2 for the test results of Embodiment 1 to Embodiment 3.

[0042] The test effect of table 2 embodiment 1-embodiment 3

[0043]

[0044] Through a l...

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Abstract

The invention discloses a group of rapid marsdenia tenacissima propagation culture media. The main formula of the group of culture media is as follows: an induction medium of MS+6-BA 2.0mg / L+NAA 0.5mg / L; an enrichment medium of MS+6-BA 1.0mg / L+NAA 0.3mg / L; a sound seedling culture medium of MS+6-BA 1.0mg / L+NAA 0.3mg / L+NH4NO3 0.66g / L+NaH2PO4 0.56g / L+K2SO4 1.78g / L+banana 100g / L; and a rooting medium of 1 / 2MS+6-BA 0.5mg / L+IAA 0.2mg / L. The induction rate is over 88 percent, the proliferation rate is high, the rooting rate is over 95 percent, the tissue culture propagation period is short and is only 64-72 days, and effective and rapid vegetative propagation of the marsdenia tenacissima is realized.

Description

technical field [0001] The invention belongs to the technical field of plant vegetative propagation, and in particular relates to a method for rapid propagation of Chinese herbal medicine Tengguan vine. Background technique [0002] Tongguan vine (Marsdenia tenocissima (Roxb) Wight et Arm) is a vine of the genus Milkweed in the Ascaridaceae family, also known as black bone vine. It was first recorded in "Southern Yunnan Materia Medica", and is widely distributed in tropical and subtropical regions of Asia. The whole plant has the effects of clearing away heat and detoxification, reducing inflammation and relieving pain, relieving cough and relieving asthma. Wuguxiaocarcin contained in Tongguanteng (C 21 - Steroidal glycosides and total alkaloids) have a strong destructive effect on the microtubule arrangement, the root of cancer cell division, and can block the mitosis of cancer cells, thereby effectively cutting off the proliferation and metastasis of cancer cells, so tha...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 韩俊杨生超颜秋燕候建凯龙光强黄鹤杨云沈雪梅杨丕琼
Owner YUNNAN AGRICULTURAL UNIVERSITY
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