Rapid marsdenia tenacissima propagation culture media

A kind of propagation medium and technology of Tongguanteng, which is applied in the field of rapid propagation of Chinese medicinal material Tongguanteng, can solve the problems of low reproductive rate of Tongguanteng seeds, susceptibility of cutting propagation seedlings, long growth cycle, etc., to achieve suitable scale production, Alleviate the pressure of market drug use and the effect of high rooting rate

Active Publication Date: 2015-04-29
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problems of low seed reproduction rate, long growth cycle, large variability, and easy susceptibility of cutting

Method used

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  • Rapid marsdenia tenacissima propagation culture media
  • Rapid marsdenia tenacissima propagation culture media

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Example 1

[0017] (1) Selection and treatment of explants

[0018] The explants are derived from the wild passerine vine plants in Mengzi County, Yunnan Province. The newly-born shoots of the passerine vines that are free from pests and diseases are selected and cut into a single bud stem segment with one bud as the explants. The preliminary sterilization is carried out in the following order: Rinse for 2.5 hours in running water, soak in 5% washing powder water for 5 minutes, and rinse for 25 minutes under running water; soak the cane segments that have been preliminarily sterilized with 75% ethanol solution for 1 minute, and then use the mass fraction After immersing in 0.1% mercuric chloride solution for 12 minutes, it is washed with sterile water for 3 to 5 times, and the water on the surface of the cane section of the pass is absorbed by sterile paper for use.

[0019] Since the twigs of the customs vine are densely covered with light yellow fluff, a lot of dust will st...

Example Embodiment

[0033] Example 2

[0034] Except for the different operations in the following steps, the operation of the other steps is the same as that of the embodiment 1, and will not be repeated.

[0035] (1) Selection and sterilization of explants

[0036] The explants were from the wild vine plants in Mengzi County, Yunnan Province, and were transplanted into the new shoots that grew out of the greenhouse of Yunnan Agricultural University after domestication. The other disinfection treatment steps are the same as the explant treatment method in Example 1, except that the disinfection time with a 0.1% mercury chloride solution with a mass fraction of 11 minutes can achieve the lowest contamination rate and a high survival rate.

Example Embodiment

[0037] Example 3

[0038] In Embodiment 3, except for the following steps, the operations are the same as those in Embodiment 1, and will not be repeated.

[0039] (1) Selection and sterilization of explants

[0040] The explants were derived from the wild tongguan rattan plants in Maguan County, Yunnan Province. The new shoots of the tongguan rattan without pests and diseases were selected and cut into a single bud stem segment with a bud as the explants. The mass fraction was 0.1% mercury chloride. When the disinfection time is 12 minutes, the pollution rate reaches the lowest and the survival rate is high. Its disinfection time is the same as that of Example 1 where the explants are from Mengzi County, Yunnan Province, which is related to the fact that they are all from the wild, and the plants have a lot of dust and germs.

[0041] The test results of Example 1 to Example 3 are shown in Table 2.

[0042] Table 2 Experimental effects of Example 1 to Example 3

[0043]

[0044] Throu...

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Abstract

The invention discloses a group of rapid marsdenia tenacissima propagation culture media. The main formula of the group of culture media is as follows: an induction medium of MS+6-BA 2.0mg/L+NAA 0.5mg/L; an enrichment medium of MS+6-BA 1.0mg/L+NAA 0.3mg/L; a sound seedling culture medium of MS+6-BA 1.0mg/L+NAA 0.3mg/L+NH4NO3 0.66g/L+NaH2PO4 0.56g/L+K2SO4 1.78g/L+banana 100g/L; and a rooting medium of 1/2MS+6-BA 0.5mg/L+IAA 0.2mg/L. The induction rate is over 88 percent, the proliferation rate is high, the rooting rate is over 95 percent, the tissue culture propagation period is short and is only 64-72 days, and effective and rapid vegetative propagation of the marsdenia tenacissima is realized.

Description

technical field [0001] The invention belongs to the technical field of plant vegetative propagation, and in particular relates to a method for rapid propagation of Chinese herbal medicine Tengguan vine. Background technique [0002] Tongguan vine (Marsdenia tenocissima (Roxb) Wight et Arm) is a vine of the genus Milkweed in the Ascaridaceae family, also known as black bone vine. It was first recorded in "Southern Yunnan Materia Medica", and is widely distributed in tropical and subtropical regions of Asia. The whole plant has the effects of clearing away heat and detoxification, reducing inflammation and relieving pain, relieving cough and relieving asthma. Wuguxiaocarcin contained in Tongguanteng (C 21 - Steroidal glycosides and total alkaloids) have a strong destructive effect on the microtubule arrangement, the root of cancer cell division, and can block the mitosis of cancer cells, thereby effectively cutting off the proliferation and metastasis of cancer cells, so tha...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 韩俊杨生超颜秋燕候建凯龙光强黄鹤杨云沈雪梅杨丕琼
Owner YUNNAN AGRICULTURAL UNIVERSITY
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