Orthotopic implantation module of mouse colorectal cancer built by mesocaecum triangle and method of orthotopic implantation module

A technology for mesocecal and colon cancer, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of long cycle and instability of colon cancer models, and achieve high incidence of liver metastases, easy control, and blood supply. rich effects

Inactive Publication Date: 2015-04-29
何向锋 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Technical problem to be solved in the present invention Aiming at the defects of long and unstable establishment period of colon canc

Method used

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  • Orthotopic implantation module of mouse colorectal cancer built by mesocaecum triangle and method of orthotopic implantation module
  • Orthotopic implantation module of mouse colorectal cancer built by mesocaecum triangle and method of orthotopic implantation module
  • Orthotopic implantation module of mouse colorectal cancer built by mesocaecum triangle and method of orthotopic implantation module

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0049] Example 1: Method for preparing mouse colon cancer in situ tumor-bearing model via cecal mesentery triangle

[0050] 1.1 Preparation of CT26.WT cell suspension

[0051] For conventional cell culture, CT26.WT cells were inoculated in RPMI-1640 medium containing 10% fetal bovine serum and placed at 37°C, 5% CO 2 Culture in a cell incubator. Take the cells in the exponential growth phase, digest them with 0.25% trypsin containing 0.02% EDTA, mechanically pipette into a cell suspension, filter with a 200 mesh screen, centrifuge at 1000 rpm for 8 minutes, discard the supernatant, wash twice with PBS, and add the cell pellet Resuspend in an appropriate amount of saline, trypan blue staining to determine cell viability>95%, adjust the cell concentration to 2.5×10 7 / ml, keep in ice bath for later use.

[0052] 1.2 After the mice were anesthetized by intraperitoneal injection of 10% chloral hydrate (0.05ml / g), they were taken in a supine position, and the mice’s limbs were non-invasi...

Example Embodiment

[0056] Example 2: Ultrasound examination of mouse colon cancer

[0057] 2.1 Method

[0058] 25 days after the operation of the mice, high-frequency ultrasound was used to observe whether there was an abnormal echo area at the cecum inoculation site, and the echo type, shape and size of the abnormal mass were recorded.

[0059] 2.2 Results

[0060] Using high-frequency ultrasound to observe the site of the cecum inoculation, there is a hypoechoic mass shadow in the abdominal cavity. The maximum diameter is between 6.1~14.5mm and the average is (9.1±0.4)mm. The test results are as follows Figure 4 Shown.

Example Embodiment

[0061] Example 3: MRI examination of mouse colon cancer

[0062] 3.1 Method

[0063] The mice with colon cancer tumor in situ were anesthetized with isoflurane gas and fixed on the scanning bed, using a 38mm rat body coil. . MR imaging adopts 7.0T Micro-MRI (Pharma Scan, Bruker, Germany, provided by Jiangsu Key Laboratory of Molecular Imaging and Functional Imaging, Southeast University), the imaging sequence and parameters are as follows: MSME-T2WI sequence: TR 2400ms, TE 16.1ms, layer Thickness is 2mm, FOV3.0cm×3.0cm, matrix 256×256, imaging time is about 3 minutes and 20 seconds.

[0064] 3.2 Results

[0065] T2WI image showed that the mouse cecum area had an oval-shaped, slightly high-intensity mass shadow of about 1.7×0.8 cm, the signal was uneven, there was a small patch of slightly low signal shadow, and the boundary of the lesion was not clear ( Figure 5 ).

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Abstract

The invention discloses an orthotopic implantation module of a mouse colorectal cancer built by a mesocaecum triangle and a method of the orthotopic implantation module. The method comprises the step of injecting a colon cancer cell suspension into the mesocaecum triangle of an anesthetic mouse to enable cancer cells to distribute and grow axially along a caecum to which a mesenterium of the somatic part of the caecum is adhered. Since a tumour inoculating part of an orthotopic bearing cancer module of the colorectal cancer is located on the mesocaecum triangle, the inoculating part has mesocaecum arteries partially, the blood supply is abundant, and s tumor is easy to grow; the intestinal wall of the part does not have serosae, therefore colorectal cancer cells are easy to grow, and the tumor cells inoculated are in direct contact with the caecum wall, therefore the tumour is formed on the caecum in situ; the enteric cavity of the caecum is wide, and bubbles formed after the injection are distributed along a mesocaecum adhering place, and the finally formed tumors are distributed along the caecum axially, therefore the death of the mouse cannot be caused easily due to enterocoel obstruction in short term; a bearing cancer module is built by injecting the cancer cell suspension, therefore the injection dose of the cancer cell is easily controlled, and the uniformity of the tumor is good.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a mouse colon cancer orthotopic tumor-bearing model inoculated in the mesenteric triangle of the cecum and a method for establishing the same. Background technique [0002] Colon cancer is a malignant tumor that seriously threatens human life and health. Distant metastasis and invasive growth are the main causes of death from human colon cancer. Modern studies have confirmed that about 60%-70% of patients have liver metastasis through autopsy of patients who died of colon cancer. Therefore, establishing an animal model of colon cancer liver metastasis that is easy to observe and whose biological behavior is closer to clinical manifestations is of great significance for understanding the process of colon cancer growth, evolution, and metastasis, and for correctly evaluating the efficacy of various treatment options. . [0003] At present, the methods commonly used to establi...

Claims

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Application Information

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IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 何向锋施文陈宝安强福林张一心蔡晶王建红沈康杨俐萍陈海珍李国兴赵文静陈橼朱烨菁
Owner 何向锋
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