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Orthotopic implantation module of mouse colorectal cancer built by mesocaecum triangle and method of orthotopic implantation module

A technology for mesocecal and colon cancer, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of long cycle and instability of colon cancer models, and achieve high incidence of liver metastases, easy control, and blood supply. rich effects

Inactive Publication Date: 2015-04-29
何向锋 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Technical problem to be solved in the present invention Aiming at the defects of long and unstable establishment period of colon cancer model, a method for preparing a high-metastasis, high-infiltration and stable mouse colon cancer orthotopic implantation model is provided

Method used

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  • Orthotopic implantation module of mouse colorectal cancer built by mesocaecum triangle and method of orthotopic implantation module
  • Orthotopic implantation module of mouse colorectal cancer built by mesocaecum triangle and method of orthotopic implantation module
  • Orthotopic implantation module of mouse colorectal cancer built by mesocaecum triangle and method of orthotopic implantation module

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Experimental program
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Effect test

Embodiment 1

[0049] Example 1: Method for preparing an orthotopic tumor-bearing model of colon cancer in mice via the mesenteric triangle of the cecum

[0050] 1.1 Preparation of CT26.WT cell suspension

[0051] For routine cell culture, CT26.WT cells were inoculated in RPMI-1640 medium containing 10% fetal bovine serum at 37°C and 5% CO 2 Cultured in a cell culture incubator. Take cells in the exponential growth phase, digest with 0.25% trypsin containing 0.02% EDTA, mechanically pipette into a cell suspension, filter through a 200-mesh screen, centrifuge at 1000rpm for 8min, discard the supernatant, wash twice with PBS, and add the cell pellet Resuspend in appropriate amount of normal saline, trypan blue staining to determine cell viability>95%, adjust cell concentration to 2.5×10 7 / ml, and stored in ice bath for future use.

[0052] 1.2 After the mice were anesthetized by intraperitoneal injection of 10% chloral hydrate (0.05ml / g), they were placed in a supine position, and the limb...

Embodiment 2

[0056] Example 2: Ultrasonic examination of colon cancer in mice

[0057] 2.1 Method

[0058] Twenty-five days after the operation, high-frequency ultrasound was used to observe whether there were abnormal echo areas at the inoculation site of the cecum, and the echo type, shape and size of the abnormal mass were recorded.

[0059] 2.2 Results

[0060] High-frequency ultrasound was used to observe the cecal inoculation site, and a hypoechoic mass shadow appeared in the abdominal cavity, with a maximum diameter ranging from 6.1 to 14.5 mm and an average of (9.1±0.4) mm. The test results were as follows: Figure 4 shown.

Embodiment 3

[0061] Example 3: MRI examination of mouse colon cancer

[0062] 3.1 Method

[0063] Mice with orthotopic colon cancer tumors were anesthetized with isoflurane gas and fixed on a scanning table, using a 38mm rat body coil. . MR imaging adopts 7.0T Micro-MRI (Pharma Scan, Bruker, Germany, provided by Jiangsu Key Laboratory of Molecular Imaging and Functional Imaging, Southeast University). The imaging sequence and parameters are as follows: MSME-T2WI sequence: TR 2400ms, TE 16.1ms, layer The thickness is 2mm, the FOV is 3.0cm×3.0cm, the matrix is ​​256×256, and the imaging time is about 3 minutes and 20 seconds.

[0064] 3.2 Results

[0065] T2WI images showed that there was an oval-shaped slightly high-intensity mass of about 1.7×0.8cm in the cecum of the mouse, the signal was uneven, and small flake-like slightly low-intensity shadows were seen inside, and the boundary of the lesion was not clear ( Figure 5 ).

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Abstract

The invention discloses an orthotopic implantation module of a mouse colorectal cancer built by a mesocaecum triangle and a method of the orthotopic implantation module. The method comprises the step of injecting a colon cancer cell suspension into the mesocaecum triangle of an anesthetic mouse to enable cancer cells to distribute and grow axially along a caecum to which a mesenterium of the somatic part of the caecum is adhered. Since a tumour inoculating part of an orthotopic bearing cancer module of the colorectal cancer is located on the mesocaecum triangle, the inoculating part has mesocaecum arteries partially, the blood supply is abundant, and s tumor is easy to grow; the intestinal wall of the part does not have serosae, therefore colorectal cancer cells are easy to grow, and the tumor cells inoculated are in direct contact with the caecum wall, therefore the tumour is formed on the caecum in situ; the enteric cavity of the caecum is wide, and bubbles formed after the injection are distributed along a mesocaecum adhering place, and the finally formed tumors are distributed along the caecum axially, therefore the death of the mouse cannot be caused easily due to enterocoel obstruction in short term; a bearing cancer module is built by injecting the cancer cell suspension, therefore the injection dose of the cancer cell is easily controlled, and the uniformity of the tumor is good.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a mouse colon cancer orthotopic tumor-bearing model inoculated in the mesenteric triangle of the cecum and a method for establishing the same. Background technique [0002] Colon cancer is a malignant tumor that seriously threatens human life and health. Distant metastasis and invasive growth are the main causes of death from human colon cancer. Modern studies have confirmed that about 60%-70% of patients have liver metastasis through autopsy of patients who died of colon cancer. Therefore, establishing an animal model of colon cancer liver metastasis that is easy to observe and whose biological behavior is closer to clinical manifestations is of great significance for understanding the process of colon cancer growth, evolution, and metastasis, and for correctly evaluating the efficacy of various treatment options. . [0003] At present, the methods commonly used to establi...

Claims

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Application Information

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IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 何向锋施文陈宝安强福林张一心蔡晶王建红沈康杨俐萍陈海珍李国兴赵文静陈橼朱烨菁
Owner 何向锋
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