Room temperature phosphorescence detection kit for detecting adriamycin content and detection method thereof
A detection kit, room temperature phosphorescence technology, applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of complex pretreatment process, poor anti-interference ability, expensive price, etc., achieve broad application prospects, low detection limit, The effect of high accuracy
Inactive Publication Date: 2015-04-29
山西师范大学
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Problems solved by technology
At present, the detection methods for doxorubicin mainly include high performance liquid chromatography, capillary electrophoresis-ultraviolet method, fluorescence method, chemiluminescence method, etc., but these methods are time-consuming, or expensive, or require complicated pretreatment processes, Or poor anti-interference ability
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Embodiment 1
[0029] A room temperature phosphorescence detection kit for detecting doxorubicin content, including reagent tubes with 4 tubes of different materials in the box, specifically:
[0030] (1) 5ml of manganese-doped zinc sulfide room temperature phosphorescent quantum dot solution wrapped in mercaptopropionic acid, with a concentration of 2g / L-6g / L;
[0031] (2) 7ml of CTAB solution with a concentration of 10 -3 -10 -2 M;
[0032] (3) 5ml of 10-[(3-amino-2,3,6-trideoxy-a-L-lyseohexapyranyl)-oxy]-7,8,9,10-tetrahydro-6,8 , 11-trihydroxy-8-(glycolyl)-1-methoxy-5,12-naphthalenedione hydrochloride standard solution, the concentration is 1-10 μM;
[0033] (4) 25ml of PBS buffer solution with a concentration of 0.2M and a pH value of 7.4.
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Abstract
The invention discloses a room temperature phosphorescence detection kit for detecting adriamycin content and a testing method thereof. The room temperature phosphorescence detection kit for detecting adriamycin content provided by the invention comprises cetyl trimethyl ammonium bromide (CTAB) and room temperature phosphorescence quantum dots. According to the method disclosed by the invention, the quantitative detection of adriamycin in serum and urine can be realized, the detection limit is low, the detection sensitivity is high, and the method is not interfered by autofluorescence of the serum and urine and also can be used for detecting the adriamycin content in drugs. Therefore, the detection kit and the detection method disclosed by the invention have wide application prospect in the field of adriamycin detection.
Description
technical field [0001] The invention relates to the technical field of detection kits, in particular to a room temperature phosphorescence detection kit and detection method for detecting the content of doxorubicin. Background technique [0002] Doxorubicin (DXR) is an anti-tumor antibiotic with a broad anti-tumor spectrum and has effects on a variety of tumors. It is a cycle non-specific drug and has a killing effect on tumor cells in various growth cycles. However, doxorubicin has certain toxic effects. The main toxic reactions are: delayed severe heart failure, leukopenia and thrombocytopenia; 100% of patients have different degrees of hair loss, nausea, loss of appetite; Cause tissue ulcers and necrosis, etc. In addition, the urine may appear red after medication. In order to grasp and control the side effects caused by doxorubicin in time, it is necessary to develop an economical and rapid method for the determination of doxorubicin content in human serum and urine. Ve...
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Login to View More IPC IPC(8): G01N21/64
Inventor 苗艳明张直峰闫桂琴
Owner 山西师范大学
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