Method for producing golden flower dark tea by employing rapid fermentation of raw dark tea brick
A technology of Jinhua dark tea and black tea hair tea, which is applied in tea treatment before extraction, etc., can solve the problems of fermentation condition requirements, high plant equipment investment, high production and operation costs, high production capacity requirements, and cumbersome fermentation procedures, etc., to achieve Improve the value and market recognition, high-efficiency flowering, and high collection value
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Embodiment 1
[0028] Obtaining the strains of S. coronis by artificial inoculation: inoculate the strains of S. coronis on the cooled activation medium, and cultivate them at 25°C for 20 hours. Put the cyst shell into a sterile vial filled with 10 mL of sterile water, shake it for 15 minutes to make a uniform spore suspension, and then perform 10-fold incremental dilution to make a dilution of 10 -3 The spore suspension was inoculated into the expansion medium at a volume ratio of 1:10, and cultured at 25°C for 18 hours at a constant temperature shaking table with a rotation speed of 100r / min to obtain the strain.
[0029] Wherein, the activation medium is prepared by the following method: 2 g / L of sodium nitrate, 1 g / L of dipotassium hydrogen phosphate, 0.4 g / L of potassium chloride, 0.5 g / L of magnesium sulfate, and 0.01 g / L of iron sulfate according to the following additions. L, sucrose 20g / L, agar 20g / L mixed, at 100 ℃, sterilized for 20min and then cooled, poured the culture bottle on...
Embodiment 2
[0038] Acquisition of artificial inoculation of S. coronis strains: inoculate the S. coronis strains on the cooled activation medium, and cultivate them at 28°C for 20 hours. After the plaques grow, pick the yellow closed Put the cyst shell into a sterile vial filled with 20 mL of sterile water, shake for 12 minutes to make a uniform spore suspension, and then perform 10-fold incremental dilution to make a dilution of 10 -2 The spore suspension was inoculated into the expansion medium at a volume ratio of 1:50, and cultured at 28°C for 20 hours at a constant temperature shaker with a rotation speed of 120r / min to obtain strains.
[0039] Among them, the activation medium is prepared by the following method: add sodium nitrate 2.5g / L, dipotassium hydrogen phosphate 1.2g / L, potassium chloride 0.6g / L, magnesium sulfate 0.6g / L, iron sulfate 0.12 g / L, sucrose 30g / L, agar 25g / L, mix at 100°C, sterilize for 15 minutes, cool down, invert the culture bottle on an inclined plane, and it...
Embodiment 3
[0048] Acquisition of artificial inoculation of S. coronis strains: Inoculate S. coronis strains on the cooled activation medium, culture at 27°C for 19 hours, and pick yellow closed cells with an inoculation loop after plaques grow. Put the cyst shell into a sterile vial filled with 15 mL of sterile water, shake for 12 minutes to make a uniform spore suspension, and then perform 10-fold incremental dilution to make a dilution of 10 -3 The spore suspension was inoculated into the expansion medium at a volume ratio of 1:25, and cultured at 27°C for 18 hours at a constant temperature shaking table with a rotation speed of 125r / min to obtain the strain.
[0049] Among them, the activation medium is prepared by the following method: add sodium nitrate 2.3g / L, dipotassium hydrogen phosphate 1.1g / L, potassium chloride 0.55g / L, magnesium sulfate 0.55g / L, iron sulfate 0.12 g / L, maltose 30g / L, agar 20g / L, mix at 121°C, sterilize for 30 minutes, cool down, pour the inclined culture bott...
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