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A method for increasing the yield of high-quality gelatin

A gelatin, high-quality technology, applied in the field of improving the yield of high-quality gelatin, can solve the problems of increasing the extraction process, unable to guarantee the completeness of the gelatin extraction, affecting the quality of the glue liquid, etc.

Active Publication Date: 2017-08-29
HUNAN ER KANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the specificity of the enzyme, a single enzymatic extraction cannot guarantee the complete extraction of the gel, and the extraction process is undoubtedly increased when the process of extracting the gel is divided into different stages (Patent Publication No. CN 101107974)
The control of the end point of enzymatic hydrolysis is also a key step in enzymatic gel extraction. The common practice is to increase the temperature to 80-100°C instantaneously (patent publication number CN 101107974) or adjust the pH of the gel solution to 1-2 (patent publication No. CN 101637216A), these will affect the quality of glue

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In this example, 1 kg of pig skin is used to prepare gelatin by the method of the present invention, including the following steps;

[0023] (1) Enzymatic hydrolysis and gel extraction: adjust the pH of the gel to 4.0 with citric acid, add 10 ppm cathepsin, and add 0.03% of the weight of the raw material, keep the temperature at 45 °C, and the reaction time is 1 hour; add calcium hydroxide solution or Ammonia water, adjust the pH to 6.0, add 30ppm neutral protease, the addition amount is 0.01% of the raw material weight, keep the temperature at 50°C, and enzymolysis for 1.0 hours; add calcium hydroxide alkali solution or ammonia water to adjust the pH of the glue solution to 7.0, the temperature rises At 50°C, the protease was inactivated, and 50 ppm of trypsin was added to the reaction, keeping the temperature at 50°C, and enzymatic hydrolysis for 2 hours.

[0024] (2) Removal of impurities: In the post-treatment process, a mixture of chitosan, sodium alginate, divinyl...

Embodiment 2

[0029] The present embodiment adopts 1 kg of cowhide to prepare gelatin by the method of the present invention, including the following steps;

[0030] (1) Enzymatic hydrolysis and gel extraction: adjust the pH of the gel solution to 5.0 with citric acid, add 10 ppm papain, and add 0.04% of the weight of the raw material, keep the temperature at 50 °C, and the reaction time is 1.5 hours; add calcium hydroxide solution or Ammonia water, adjust the pH to 7.0, add 30ppm of neutral protease, the addition amount is 0.02% of the raw material weight, keep the temperature at 55 ° C, and enzymolysis for 1.5 hours; add calcium hydroxide alkali solution or ammonia water to adjust the pH of the glue solution to 7.5, the temperature rises At 55°C, the protease was inactivated, and 50 ppm of trypsin was added to the reaction, maintaining the temperature at 55°C, and enzymatic hydrolysis for 2.5 hours.

[0031] (2) Removal of impurities: In the post-treatment process, a mixture of chitosan, ...

Embodiment 3

[0036] In the present embodiment, 10 kg of ossine is used to prepare gelatin by the method of the present invention, including the following steps;

[0037] (1) Enzymatic hydrolysis and gel extraction: adjust the pH of the gel solution to 6.5 with citric acid, add 10ppm papain, add 0.05% of the weight of the raw material, keep the temperature at 55 °C, and the reaction time is 2 hours; add calcium hydroxide solution or Ammonia water, adjust the pH to 8.0, add 30ppm of neutral protease, the addition amount is 0.03% of the raw material weight, keep the temperature at 60°C, enzymolysis for 2 hours; the temperature rises to 60°C to inactivate the protease, add calcium hydroxide alkali solution Or ammonia water to adjust the pH of the glue solution to 8.0, add 50 ppm trypsin for the reaction, keep the temperature at 60 °C, and enzymatic hydrolysis for 3 hours.

[0038] (2) Removal of impurities: in the post-treatment process, add chitosan, sodium alginate, divinylbenzene cross-link...

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Abstract

The invention discloses a method for improving the yield of high-quality gelatin, which comprises the steps of adjusting the pH of the glue obtained in the pretreatment process, adding biological protease in stages to extract the glue, and adding bamboo charcoal-chitosan composite into the obtained glue The gelatin product is obtained by adsorption and filtration, bleaching and sterilization with hydrogen peroxide, impurity removal and concentration by membrane method, and finally drying on a straight plate. The advantages of this method are that (1) the use of segmented enzymatic extraction can make collagen hydrolysis more complete, and the gelatin yield can be greatly improved; (2) after bamboo charcoal-chitosan complex adsorption filtration, H2O2 treatment, membrane method The steps of removing impurities and concentrating can obtain gelatin products with high viscosity and high freezing power.

Description

technical field [0001] The invention relates to a method for improving the yield of high-quality gelatin, and belongs to the technical field of gelatin making. . Background technique [0002] Gelatin is a polymer compound, and its main raw materials are animal skin, bone, cartilage, ligament, sarcolemma, etc. The traditional methods of extracting gelatin are acid method and alkali method. Since the 1950s, people have been trying to introduce the degradation of collagen by protease into gelatin production. Compared with the traditional process, it has the advantages of short cycle and easy equipment. control and less environmental pollution. [0003] At present, enzymatic extraction is divided into single enzymatic extraction and combined enzymatic extraction. Due to the specificity of enzymes, single enzymatic extraction cannot guarantee complete extraction of gum, and the extraction process is undoubtedly increased when the extraction process is divided into different st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 帅放文王向峰章家伟
Owner HUNAN ER KANG PHARMA
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