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Application of carbonic anhydrase IV to preparing lung adenocarcinoma diagnostic preparation

A carbonic anhydrase and lung adenocarcinoma technology, applied in the field of biomedicine, can solve the problems of complex and variable influencing factors of the regulatory mechanism, and the mechanism remains to be elucidated.

Active Publication Date: 2015-05-20
FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, because various regulatory mechanisms in the body are complex and changeable, and there are many influencing factors, although research on the molecular mechanism of lung adenocarcinoma has been carried out and many new progresses have been made, the exact mechanism has yet to be elucidated, and it is likely to involve multiple factors. pathway involved in regulation

Method used

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  • Application of carbonic anhydrase IV to preparing lung adenocarcinoma diagnostic preparation
  • Application of carbonic anhydrase IV to preparing lung adenocarcinoma diagnostic preparation
  • Application of carbonic anhydrase IV to preparing lung adenocarcinoma diagnostic preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Meta-analysis of high-throughput transcriptome data in lung adenocarcinoma

[0027] The GEO (Gene Expression Omnibus) database is developed and maintained by NCBI (National Center for Biotechnology Information). The GEO database is the largest database of gene expression data. SAGE (Serial Analysis of Gene Expression) data, SARST (Serial Analysis of Ribosomal Sequence Tags) data, MS (Mass Spectrometry) data, proteome data and next-generation high-throughput sequencing data (MPSS, massively parallel sequencing technology), etc. The search keywords were ("lung adenocarcinoma" [MeSH Terms] OR "lung adenocarcinoma" [All Fields]) AND "Homo sapiens" [porgn]. Limit the research type to "expression profiling by array" Data sets that meet the following criteria will be included in our research: ①The selected data set must be genome-wide expression mRNA transcriptome data ②These data come from lung adenocarcinoma case groups and controls The biopsy tissue or cultured c...

Embodiment 2

[0032] Example 2 Lung adenocarcinoma tissue and normal tissue RAMP3 and CA4 gene expression

[0033] 1. Materials and methods

[0034] 1. Materials

[0035] Collect 5 cases of lung adenocarcinoma tissue and 5 cases of normal tissue, group and number them.

[0036] 2. Method

[0037] 2.1 Extraction of total RNA from lung adenocarcinoma tissue and normal tissue

[0038] use Reagent (invitrogen, Cat. No. 15596-018) was used to extract sample RNA, and the experimental operation was carried out according to the product manual. The specific operation was as follows:

[0039] After collecting the samples, freeze them in liquid nitrogen. After taking them out, put the tissue into a pre-cooled mortar for grinding. After the tissue sample is powdered:

[0040] ① Add Trizol and store at room temperature for 5 minutes;

[0041] ② Add 0.2ml of chloroform, vibrate the centrifuge tube vigorously, mix well, and place it at room temperature for 5-10 minutes;

[0042] ③ After 15 minutes...

Embodiment 3

[0066] Example 3 CA4 gene expression in lung adenocarcinoma tissue and paracancerous tissues

[0067] 1. Materials and methods

[0068] 1. Materials

[0069] Non-small cell lung cancer was collected from hospitalized cases from 2005 to 2010. 134 lung adenocarcinoma tissues and adjacent tissues were collected and numbered separately.

[0070] 2. Method

[0071] Refer to the experimental method in Example 2.

[0072] two results

[0073] Among the 116 samples of lung adenocarcinoma, the expression level of CA4 in the cancer tissue was lower than that in the adjacent tissue in 115 samples, and the expression level of CA4 in the cancer tissue was not clearly different from that in the adjacent tissue in only one sample. The ratio is 99%, indicating that the CA4 gene has a good correlation with lung adenocarcinoma, and can be used to prepare preparations for auxiliary diagnosis or prognosis of lung adenocarcinoma.

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Abstract

The invention relates to an application of carbonic anhydrase IV to preparing a lung adenocarcinoma diagnostic preparation. Meta analysis is performed on high-throughout transcriptome data of the lung adenocarcinoma to obtain a differential expression gene, and an analysis tool is applied to analyzing functions of the differential expression gene, so that a candidate gene is screened, and the correlation between the carbonic anhydrase IV and the lung adenocarcinoma is verified by applying a classical molecular biological method; the carbonic anhydrase IV can be used for preparing an auxiliary lung adenocarcinoma diagnostic preparation, and has an important clinical application value.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the new application of carbonic anhydrase IV in the preparation of lung adenocarcinoma diagnostic preparations. Background technique [0002] Carbonic anhydrase (CA) is a kind of metalloenzyme containing zinc, its function is to catalyze the hydration of carbon dioxide and the hydrolysis of carbonic acid, and it plays an important role in regulating the transportation of carbon dioxide, water electrolyte and pH balance. At least 15 different types of carbonic anhydrase isoenzymes have been found so far, among which carbonic anhydrase III and IV are relatively active, and carbonic anhydrase IV mainly exists in adipose tissue, liver, cardiac muscle and slow skeletal muscle fibers. Antibodies have been detected in patients with systemic lupus erythematosus, rheumatoid arthritis, and diabetes, and type IV anti-carbonic anhydrase antibodies play an important role in the development of humor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/574G01N33/573
CPCC12Q1/6886G01N33/573G01N33/57423
Inventor 田子强王贵英张月峰
Owner FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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