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Dengue virus rapid classification identification detection kit

A dengue virus, identification and detection technology, applied in the determination/inspection of microorganisms, resistance to vector-borne diseases, microorganisms, etc., can solve the problems of immune cross-reaction cycle, low sensitivity, easy pollution, etc., to achieve simple operation, high-pass amount, the effect of reducing pollution

Inactive Publication Date: 2015-05-20
GUANGZHOU EIGHTH PEOPLES HOSPITAL +1
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

It has the disadvantages of low sensitivity, immune cross-reaction, and long cycle; while conventional PCR also has the disadvantage of easy contamination

Method used

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  • Dengue virus rapid classification identification detection kit
  • Dengue virus rapid classification identification detection kit
  • Dengue virus rapid classification identification detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 dengue virus typing identification detection kit and its use

[0033] 1. Prepare a kit including the following components: 1 tube of primer-probe mixture (50 μl / tube), amplification reaction buffer (375 μl / tube), 1 tube of amplification reaction enzyme system (75 μl / tube), positive Control product (200μl / tube) 1 tube, negative quality control product (200μl / tube) 1 tube.

[0034] 2. Collection, preservation and transportation of specimens

[0035] 2.1 Specimen types: serum and plasma samples.

[0036] 2.2 Specimen collection:

[0037] 2.2.1 Serum: Aseptically collect 3-5ml of venous blood within 5 days after the onset of the disease, let it stand at room temperature for 30 minutes to solidify, then centrifuge at 1500-2000rpm for 10min to remove fibrin and red blood cells, and collect serum in 2ml of sterile screw-top plastic tube.

[0038] 2.2.2 Plasma: Aseptically collect blood samples and place them in a test tube containing an appropriate amount of a...

Embodiment 2

[0050] Example 2 Application of Dengue Virus Type Identification and Detection Kit to Detect Clinical Samples

[0051] One case of positive serum specimens identified as dengue virus type I, dengue virus type II, dengue virus type III, and dengue virus type IV were selected by the virus culture method, and one case was identified as Chikungunya virus by the virus culture method , Japanese encephalitis virus, fever with thrombocytopenia syndrome bunya virus, Hantaan virus, and Seoul virus positive serum samples were used as specific samples, and all samples were subjected to nucleic acid extraction, PCR amplification and result analysis steps Carry out with reference to embodiment 1, carry out the detection of negative, positive quality control product simultaneously.

[0052] Test result: the amplification curve of the negative quality control product is not S-shaped (see attached figure 2 ), the amplification curve of the positive quality control product is an obvious S-sha...

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Abstract

The invention relates to a dengue virus rapid classification identification detection kit, and in particular relates to a detection kit used for identifying four serum types of the dengue virus by using the multiple real-time fluorescent rapid polymerase chain reaction technology, namely, I type, II type, III type and IV type. The kit mainly comprises an amplification reaction buffer liquid, a primer probe reaction liquid, an amplification reaction enzyme system, a negative quality control product, a positive quality control product, and a packing box used for packaging the reagent bottles or tubes in a separation and centralized package mode. The kit adopts the multiple fluorescent channel respective detection mode, the Taq enzyme having rapid polymerization and the one-step real time rapid PCR reaction mode for exactly detecting whether the sample has the dengue virus nucleic acid and identifying the four serum types of the dengue virus; the dengue virus rapid classification identification detection kit is widely used for the early differential diagnosis, epidemic prevention and control, inspection and quarantine and other fields for the dengue, especially the severe dengue fever and mixed infection dengue virus.

Description

technical field [0001] The invention relates to a kit for rapid identification and detection of dengue virus serotypes, in particular to a kit for identifying four dengue virus serotypes in one tube by using rapid multiple real-time fluorescent polymerase chain reaction technology . This kit mainly includes amplification reaction buffer, primer probe reaction solution, amplification reaction enzyme system, negative quality control product, positive quality control product, and packaging boxes for separating and collectively packaging these reagent bottles or tubes. Since this kit can accurately distinguish dengue virus types I, II, III, and IV, it can be widely used in early clinical diagnosis of dengue fever, early warning of severe cases, port inspection and quarantine, epidemic prevention and control, vector control and scientific research, etc. fields. Background technique [0002] Dengue fever and dengue hemorrhagic fever are acute vector-borne diseases caused by four...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
CPCC12Q1/686C12Q1/701C12Q2600/16C12Q2537/143C12Q2561/113C12Q2563/107Y02A50/30
Inventor 张复春高秀洁周其伟李丽梅
Owner GUANGZHOU EIGHTH PEOPLES HOSPITAL
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