Chitosan oligosaccharide compounded preparation and application in prolonging shelf life of fruits

[0018] The preparation of the low-concentration compound preparation commonly used in Example 1

[0019] The active ingredients of this compound preparation are chitosan oligosaccharides, organic acids and growth inhibitors, wherein the organic acids include tartaric acid, oxalic acid, acetic acid, lactic acid, malic acid, citric acid, citric acid, ascorbic acid, benzoic acid, salicylic acid, Caffeic acid, phytic acid, amino acids, etc.; the growth inhibitors include gibberellin, 2,4-D, carotene, penicillin, methyl tallow acetate, brassinolide, naphthalene acetic acid, jasmonic acid and its methyl esters Ester, aminoethoxyvinylglycine (AVG), aminocaproic acid (AOA), sodium benzoate, ortho-nitrophenol (PNP), polyamine, 1-methylcyclopropene (1-MCP), etc.; adding well-known Solvents and auxiliary agents are formulated into water, emulsifiable concentrate, soluble liquid, water emulsion, microemulsion and suspension. The present embodiment has introduced a kind of commonly used water agent proportioning, but the content of the present invention is not limited to this:

[0020] 1 g of chitosan oligosaccharide is dissolved in 100 ml of acetic acid solution with a mass concentration of 0.6%, and then pure gibberellin with a final mass concentration of 0.2% is added to make it completely dissolved, thereby preparing the chitosan oligosaccharide compound preparation.

[0021] The contents of the three main components were determined by high performance liquid chromatography, and the chromatographic conditions for the determination of gibberellin were: μ-Band park C18 column; methanol: water (containing 5% glacial acetic acid) (55:45, V/V) ); flow rate 0.8ml/min; detection wavelength 252nm; chromatographic conditions for acetic acid determination: ODS C18 column (4.6mm×250mm, 5μm), with 0.1% phosphoric acid aqueous solution as mobile phase, flow rate 1.0ml/min, detection wavelength 210nm, The column temperature was 25°C. The chromatographic conditions for the determination of chitosan oligosaccharides are: Shodex NH2p-504E amino column (Asahipak) (4.6×250mm); acetonitrile (A) and aqueous solution of 3‰ ammonia water (pH10, B) are used as mobile phases; column temperature is 30°C; flow rate 1.0ml/min; linear gradient elution, the elution procedure is: 25%B (0-5min)—25%-50%B (5-40min)—100%B (40-50min, cleaning the chromatographic column)— 25%B (50-60min, equilibrate column). The injection volume of the standard solution and the sample is 50 μl and 20 μl respectively. The chitosan oligosaccharide (top) and the chitosan oligosaccharide standard (bottom) with a degree of polymerization of 1-6 used in the compound preparation are shown in the chromatogram. figure 1.

[0022] Example 2 The shelf life of cherry tomatoes can be prolonged by using this compound preparation

[0023] The test was carried out in 2012, and two treatments were designed: treatment 1, control, no treatment; treatment 2, soaked cherry tomatoes after picking with the compound preparation of Example 1 for 3 minutes, air-dried and stored. After 7 days, the weight loss rate, total acid content, total sugar content and vitamin C (Vc) content were counted. The specific results are shown in Table 1.

[0024] Table 1. The effect of using this compound preparation on the weight loss rate, total acid, total sugar and Vc content of cherry tomatoes stored for 7 days

[0025] deal with

[0026] Example 3 The shelf life of star fruit can be extended by using this compound preparation

[0027] The test was carried out in 2012, and two treatments were designed: treatment 1, control, no treatment; treatment 2, soaking the picked star fruit with the compound preparation of Example 1 for 3 minutes, air-dried and stored. After 12 days, the weight loss rate, total sugar content and Vc content were counted. The specific results are shown in Table 2.

[0028] Table 2. The effect of using this compound preparation on the weight loss rate, total sugar and Vc content of carambola stored for 12 days

[0029] deal with