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Compound with plant pathogenic fungus resistant activity and preparation method of compound

A technology of phytopathogenic fungi and compounds, applied in the field of compounds with activity against phytopathogenic fungi and their preparation, can solve problems such as negative impacts on the environment and human health, and achieve industrialized large-scale production, easy operation and implementation, and good practicality value effect

Active Publication Date: 2015-05-27
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it turns out that synthetic pesticides have had enormous negative impacts on the environment and human health

Method used

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  • Compound with plant pathogenic fungus resistant activity and preparation method of compound
  • Compound with plant pathogenic fungus resistant activity and preparation method of compound
  • Compound with plant pathogenic fungus resistant activity and preparation method of compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Compound Fermentation:

[0036]1) Inoculate Bacillus subtilis (Bacillus subtilis) Pc3 in 5mL LB medium (in g / L: yeast extract 5, peptone 10, sodium chloride 10) for activation culture, at 28°C, 180r / min Cultivate on a shaking table for 12 hours;

[0037] 2) Inoculate the activated culture solution of Bacillus subtilis (Bacillus subtilis) Pc3 in step 1), the inoculation volume is 5mL, inoculate in 1L Erlenmeyer flask, each bottle contains 500mL fermentation medium (in g / L: Glucose 20, KH 2 PO 4 6. K 2 HPO 4 14. MgSO 4 0.2, (NH4) 2 SO 4 4. Trace elements 2mL (FeSO 4 4H 2 O, CaCl 2 2H 2 O, MnSO 4 4H 2 O and ZnCl 2 1 mM each), cultured on a shaking table at 28° C. and 180 r / min for 48 hours to obtain a fermentation broth containing the compound having anti-phytopathogenic fungal activity of the present invention.

Embodiment 2

[0039] Compound preparation:

[0040] Centrifuge the fermentation culture liquid for 10 min under the condition of 8000r / min to obtain the fermentation supernatant, and precipitate the fermentation supernatant containing the compound having anti-phytopathogenic fungal activity of the present invention with 3 times the volume of absolute ethanol, and use an equal volume of Ethyl acetate was extracted 3 times, and the obtained extract was concentrated. The obtained ethyl acetate part was separated by reversed-phase high-performance liquid chromatography. The chromatographic column was a Shim-Pack RPC-ODS column (20mm×25cm; No.2282346493), and the detection wavelength was 214nm. The mobile phase used was methanol-water system at 8.0 mL / min flow rate for elution, the elution program is methanol 0 ~ 20min, 5% ~ 70%; 20 ~ 30min, 70% ~ 100%; 30 ~ 35min, 100% ~ 5% Chromatographic peak; then through reverse high performance liquid chromatography with 8.0mL / min 55% methanol isocratic e...

Embodiment 3

[0042] Compound Identification:

[0043] The present invention has anti-phytopathogenic fungal compound molecular formula according to mass spectrometry HR-ESI-MS + presumed to be C 11 h 12 N 2 o 2 ([M+Na] + 227.0792) such as figure 1 shown.

[0044] The NMR data and signal assignments are shown in Table 1.

[0045] Table 1 is the nuclear magnetic resonance data of the compound with anti-phytopathogenic fungus activity of the present invention

[0046]

[0047] according to 13 C-NMR and DEPT spectra show that its structure has 11 carbons, 1 methylene, 6 methines, and 4 quaternary carbons. According to the peak type of proton and H-C(7) and C(9), C(10); H-C(8) and C(9); H-C(9) and C(7), C(10); H-C(10) Long range correlation with C(4); H-C(6) and C(7), C(8); H-C(5) and C(1), C(2), C(8), C(9) , and H-C(7) and H-C(8); H-C(8) and H-C(7), H-C(10); H-C(9) and H-C(4); H-C(10) and H-C(8) have 1 H- 1 The relationship of H COZY, it can be speculated that the indole compos...

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Abstract

The invention discloses a compound with plant pathogenic fungus resistant activity and a preparation method of the compound, relating to the field of compounds prepared by antarctic bacteria fermentation cultivation. The compound with plant pathogenic fungus resistant activity has a chemical formula C11H12N2O2. The preparation method comprises the following steps: (1) inoculating antarctic bacteria into an LB culture medium for performing activating culture; (2) inoculating antarctic bacteria subjected to activating culture in the step (1) into a fermentation medium for performing fermentation cultivation, thereby obtaining fermentation cultivation solution inoculated with antarctic bacteria; and (3) centrifuging the fermentation cultivation solution in the step (2) to obtain the fermentation supernatant, separating and purifying the fermentation supernatant, thereby obtaining the compound with plant pathogenic fungus resistant activity. The compound with plant pathogenic fungus resistant activity has broad spectrum plant pathogenic fungus resistant activity and has good application prospects in the aspect of biological control of plant diseases. The preparation method is easy to operate and implement, is easy for large-scale industrial production and has good practical value.

Description

technical field [0001] The invention relates to the field of compounds prepared by fermentation and culture of Antarctic bacteria, in particular to a compound with anti-phytopathogenic fungal activity and a preparation method thereof. Background technique [0002] Plant pathogens are the number one killer of major food and commercial crops. In the past 40 years, the control of plant diseases has mainly relied on the heavy use of synthetic pesticides. But it turns out that synthetic pesticides have had enormous negative impacts on the environment and human health. At the same time, the high cost of developing new synthetic pesticides and the rapid emergence of drug-resistant microorganisms have aroused our attention to biological pesticides. As the main body of the biopesticide market, microbial pesticides have the advantages of broad antibacterial spectrum, diversified active functions and rich active substances. From the perspective of the process of new drug development...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D209/18A01N43/38A01P3/00C12P17/10C12R1/125
CPCA01N43/38C07D209/18C12P17/10
Inventor 陈新华郭文斌崔鹏飞
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES