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A cell and nutrient technology, applied in the field of microorganisms, to achieve the effect of low mutation rate, simple formulation and rapid cell growth
Inactive Publication Date: 2015-05-27
刘汉斌
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Problems solved by technology
[0004]The composition of animal serum is complex, various biological and large molecules are mixed together, and some components have not yet been clarified
Serum is very effective for cell growth, but it will cause some difficulties in the separation, purification and detection of cultured products in the later stage
In addition, the sources of high-quality animal serum are limited and the cost is high, which limits its large-scale use
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Embodiment 1
[0015] A MEM cell culture medium, the components of the MEM cell culture medium are 200.00 mg of anhydrous calcium chloride, 93.50 mg of anhydrous magnesium sulfate, 400.00 mg of potassium chloride, 6800.00 mg of sodium chloride, and Anhydrous sodium dihydrogen phosphate 115.00 mg, L-arginine hydrochloride 126.00 mg, L-cystine hydrochloride 31.40 mg, L-histidine hydrochloride 42.00 mg, L-isoleucine 52.00 mg, L-leucine 52.00 mg, L-lysine hydrochloride 73.00 mg, L-methionine 15.00 mg, L-tyrosine 36.00 mg, L-valine 40.00 mg, choline bitartrate 18.00 mg, folic acid 3.00 mg, inositol 2.00 mg, niacinamide 1.00, D-calcium pantothenate 3.00 mg, pyridoxine hydrochloride 1.00 mg, riboflavin 0.50 mg, thiamine hydrochloride 2.00 mg, glucose 1000.00 mg, succinic acid 75.00 mg, L-phenylalanine 32.00 mg , Kanamycin 60.00 mg.
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Abstract
The invention belongs to the field of microbes and relates to a MEM cell culture medium. Each liter of the MEM cell culture medium comprises 200.00mg of anhydrous calcium chloride, 93.50mg of anhydrous magnesium sulfate, 400.00mg of potassium chloride, 6800.00mg of sodium chloride, 115.00mg of anhydrous sodium dihydrogen phosphate, 126.00mg of L-arginine hydrochloride, 31.40mg of L-cystine hydrochloride, 42.00mg of L-histidine hydrochloride, 52.00mg of L-isoleucine, 52.00mg of L-leucine, 73.00mg of L-lysine hydrochloride, 15.00mg of L-methionine, 36.00mg of L-tyrosine, 40.00mg of L-valine, 18.00mg of choline bitartrate, 3.00mg of folic acid, 2.00mg of inositol, 1.00mg of nicotinamide, 3.00mg of D-calcium pantothenate, 1.00mg of pyridoxine hydrochloride, 0.50mg of riboflavin, 2.00mg of thiamine hydrochloride, 1000.00mg of glucose, 75.00mg of succinic acid, 32.00mg of L-phenylalanine and 60.00mg of kanamycin. The MEM cell culture medium has the advantages of simple formula, convenient preparation and operation, low cost, popularization feasibility, fast cell growth, difficult pollution and low variation rate.
Description
technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a MEM cell culture medium. Background technique [0002] Cells (English name: CELL). There is no uniform definition of cells. In recent years, it has been more common to say that cells are the basic structural and functional units of organisms. It is known that all organisms except viruses are composed of cells, but the life activities of viruses must also be reflected in cells. Generally speaking, most microorganisms such as bacteria and protozoa are composed of one cell, that is, unicellular organisms; higher plants and higher animals are multicellular organisms. Cells can be divided into two types: prokaryotic cells and eukaryotic cells. However, it was also proposed that it should be divided into three categories, that is, the ancient karyocytes that originally belonged to prokaryotic cells should be separated as a parallel category. The study of cells is call...
Claims
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Application Information
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