Method for measuring contents of palmitic acid and oleic acid in polyrhachis vicina roger

A technique for the determination of the content of palmitic acid and oleic acid in the pseudo-black spiny ant, which is applied to the field of determination of the content of palmitic acid and oleic acid in the pseudo-black spiny ant, and can solve the problem that there is no method for measuring the content of palmitic acid and oleic acid in the pseudo-black spiny ant. Incomplete conversion, measurement deviation and other problems, to achieve good accuracy and repeatability, high accuracy, good repeatability

Inactive Publication Date: 2015-05-27
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

The main components of the fat-soluble components in P. black spiny ants are palmitic acid and oleic acid. Both palmitic acid and oleic acid are long-chain fatty acids with poor volatility. If the content is determined directly by gas chromatography, the It is possible that the gasification is not complete, resulting in deviation in measurement
[0004] At present, there is no research report on the content determination of palmitic acid and oleic acid in P.

Method used

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  • Method for measuring contents of palmitic acid and oleic acid in polyrhachis vicina roger
  • Method for measuring contents of palmitic acid and oleic acid in polyrhachis vicina roger
  • Method for measuring contents of palmitic acid and oleic acid in polyrhachis vicina roger

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Embodiment 1

[0041] The assay method of palmitic acid and oleic acid content in the black spiny ants, is characterized in that, comprises the steps:

[0042] (1) Preparation of internal standard stock solution:

[0043] Take 250 mg of n-octadecane, accurately weigh it, place it in a 100 ml volumetric flask, add 60-90 ° C petroleum ether to dissolve and dilute to 100 ml, and obtain the internal standard stock solution;

[0044] (2) Preparation of reference solution:

[0045] A) Accurately weigh 100mg of methyl palmitate and 100mg of methyl oleate as reference substances, put them in the same 25ml volumetric flask, dissolve and dilute to 25ml with 60-90℃ petroleum ether, shake well, and obtain the reference substance stock solution;

[0046] B) Accurately measure 2ml of the reference substance stock solution, put it in a 25ml volumetric flask, dissolve it with petroleum ether at 60-90°C and dilute it to 25ml, shake well, and obtain the reference substance solution;

[0047] (3) Preparation o...

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Abstract

The invention relates to a method for measuring contents of palmitic acid and oleic acid in polyrhachis vicina roger. The method is characterized by comprising the following steps: carrying out methyl esterification on the palmitic acid and the oleic acid with methanol under base catalysis, and carrying out a gas chromatographic method, wherein a chromatographic column is used as a Rt*R-5 capillary chromatographic column, and the specification of the chromatographic column is 30*0.32mm*0.25 micron; and heating up the column temperature from 80 DEG C to 220 DEG C in a program heating manner at a speed of 15 DEG C / min, and maintaining the temperature for 5 minutes, wherein the column flow is 2.0ml / min, carrier gas is high-purity helium, the temperature of an injection opening is 230 DEG C, the sample injection size is 1 microliter, and the split ratio is 10 to 1. The method provided by the invention is high in measurement result accuracy, simple and convenient to operate and good in repeatability, and the method can be used for measuring the contents of the palmitic acid and the oleic acid in the polyrhachis vicina roger.

Description

technical field [0001] The invention relates to a method for determining the content of specific components in medicinal insects, in particular to a method for determining the content of palmitic acid and oleic acid in the ants of the ants. Background technique [0002] Pseudomonas spp. (Poryrhaehis vicina Roger) It is the dry worm body of the medicinal insect Pseudomonas spp. Recorded in "Chinese Materia Medica", "Dictionary of Chinese Medicine", etc., produced in Guangxi, Yunnan and other places. Its nature is flat, slightly warm, tastes salty and sour, slightly fishy. Pseudomonas spinosa is rich in zinc, selenium, manganese, copper, iron and other elements B1, B2, B6, A, E and 28 essential amino acids for the human body. It has pharmacological effects such as reducing swelling and pain, anti-rheumatism, sedation, anti-inflammation, lowering fat, lowering blood sugar, anti-fatigue, relieving pain and sedation, improving sleep, and inhibiting tumors. [0003] Studies h...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 陆国寿韦桂宁刘吉成黄建猷卢文杰谭晓黄周峰何飞苏华曾宪彪韦宝伟
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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