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Aquaculture water body cleaning agent and preparation method thereof

An aquaculture and purifying agent technology, applied in the field of environmental biology, can solve the problems of secondary pollution, slow water purification, death due to incompatibility with the environment, etc., and achieve the effect of reducing purification costs

Active Publication Date: 2015-06-03
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A small amount of aquaculture sewage enters water bodies such as rivers and lakes. After the self-purification of the water body, it is easy to eliminate pollution. However, if the organic matter and NO in the aquaculture water body 3 - -N and NO 2 - The concentration of -N and other substances is too high, and it is difficult for microorganisms in the water body to degrade in a short period of time. A large amount of aquaculture sewage is not treated, and direct discharge will cause pollution to river and lake water systems. The surrounding aquaculture caused
[0004] In the practice of water purification in aquaculture water, physical adsorption, filtration and other methods are often used, but the amount of water treated by this method is limited, which limits its application in large-scale aquaculture water; adding substances to the water body to modify, exchange, flocculate and precipitate pollutants Such chemical methods, due to the addition of additional chemical substances, are likely to cause secondary pollution, which also restricts the large-scale application of this type of method
By adding microorganisms that can degrade organic matter or microorganisms that have denitrification to the aquaculture water body, although the degree of pollution can be effectively reduced, the microorganisms such as algae and bacteria added to the water body will die naturally during their own growth and reproduction life cycle Or die without adapting to the environment, the cracked bacteria will once again release various organic substances into the water body
The metabolism of the purified strain is also easily affected by the organic matter and NO in the water around the strain. 3 - -N or NO 2 - -The influence of the concentration of N, resulting in slow water purification

Method used

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  • Aquaculture water body cleaning agent and preparation method thereof
  • Aquaculture water body cleaning agent and preparation method thereof
  • Aquaculture water body cleaning agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Preparation of aquaculture water purification agent.

[0023] A preparation method of an aquaculture water body purifying agent, comprising the steps of:

[0024] (1) Inoculate B.amyloliquefaciens IAE into liquid seed medium for cultivation. The cultivation conditions are: cultivation temperature 37°C, shaker rotation speed 130-160rpm, cultivation time 24 hours. The obtained Bacillus amyloliquefaciens IAE seed liquid contains bacteria Amount greater than 1×10 9 cfu ml -1 ; The formula of the liquid fermentation medium used is prepared according to 1L medium: beef extract 3g, peptone 10g, NaCl 3g, tap water 1000ml, pH range 7.0-7.5, sterilized at 121°C for 20min;

[0025] (2) The Bacillus amyloliquefaciens IAE seed liquid that step (1) obtains is inoculated in the secondary fermentation medium according to the ratio of 5% of the secondary liquid fermentation medium volume, and the condition of fermentation production is: culture temperature 28~35 ℃, the diss...

Embodiment 2

[0027]Example 2 Preparation of aquaculture water purification agent.

[0028] A preparation method of an aquaculture water body purifying agent, comprising the steps of:

[0029] (1) Inoculate B.amyloliquefaciens IAE into liquid seed medium for cultivation. The cultivation conditions are: cultivation temperature 37°C, shaker rotation speed 130-160rpm, cultivation time 24 hours. The obtained Bacillus amyloliquefaciens IAE seed liquid contains bacteria Amount greater than 1×10 9 cfu ml -1 ; The formula of the liquid fermentation medium used is prepared according to 1L medium: beef extract 3g, peptone 10g, NaCl 3g, tap water 1000ml, pH range 7.0-7.5, sterilized at 121°C for 20min;

[0030] (2) The Bacillus amyloliquefaciens IAE seed liquid that step (1) obtains is inoculated in the secondary fermentation medium according to the ratio of 5% of the secondary liquid fermentation medium volume, and the condition of fermentation production is: culture temperature 28~35 ℃, the disso...

Embodiment 3

[0032] Example 3 Application of in-situ purification of aquaculture water purification agent.

[0033] The water body selected for the experiment is located in an aquaculture farm in Nantong, Jiangsu Province. The main culture is Penaeus vannamei. Three treatments are set up, namely:

[0034] (1) Control treatment, the water body does not use purifying agent;

[0035] (2) Market purifying agent treatment, this product is made with Bacillus subtilis, Rhodopseudomonas palustris and Chromobacterium denitrificans, every mu of aquaculture pond uses this purifying agent 4000g each time, adopts and culture water 1: 1000 volume After mixing in proportion, evenly sprinkle it into the breeding pond;

[0036] (3) For the product of the present invention, the product and the aquaculture water body are mixed according to the volume ratio of 1:1000, and the product of the present invention is evenly sprayed on the surface of the aquaculture water body in the water town by using an electric...

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Abstract

The invention discloses an aquaculture water body cleaning agent which comprises fermentation broth of bacillus amyloliquefaciens IAE, rice hull powder and calcium carbonate powder, wherein the mass ratio of the fermentation broth to the rice hull powder and the calcium carbonate powder is 2:(1-2):(1-2), and the preservation number of the strain of bacillus amyloliquefaciens IAE is CCTCC NO:M2013086. The number of the effective strains bacillus amyloliquefaciens IAE in the cleaning agent is greater than or equal to 3*10<8>cfu.g<-1>, and the content of effective flocculation substances is greater than 15g.kg<-1> by dry weight. The experiment shows that when being tested in the next day after the cleaning agent is sprayed to a penaeus vannamei culture water body, the COD is reduced by 65.2%, NH4<+>-N, NO3<->-N and NO2<->-N are respectively reduced by 60.1%, 73.7% and 78.8%, and the concentration of COD, NH4<+>-N, NO3<->-N and NO2<->-N of the water body 30 days later after heterotopic purification of the culture water body is very close to that of a non-culture natural environment water body, so that the water replacing times can be reduced, or even the water does not need to be replaced.

Description

technical field [0001] The invention belongs to the field of environmental biotechnology, and in particular relates to an aquaculture water purifier and a preparation method thereof. Background technique [0002] At present, the aquaculture area in our country reaches millions of hectares. Due to the high-density aquaculture, a large amount of feed and antibiotics need to be added to the water body during the aquaculture process. Feed residues and metabolites of cultured organisms will increase the COD of the water body; the decomposition of a large amount of feed residues, feces and other organic matter in the water body will reduce the oxygen content of the water body; and due to the ammoniation and nitrification of microorganisms in the water body, the input The nitrogen element in the protein, amino acid and other substances in the added feed will form NH 4 + -N, NO 3 - -N and NO 2 - -N and other substances, which lead to the deterioration of the aquaculture water ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
Inventor 邵雅璐陈立华王众毛俊祺佟润泽邵孝侯
Owner HOHAI UNIV