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A method for determining the content of cyclic adenosine monophosphate in jujube

A technique for cyclic adenosine monophosphate and cyclic adenosine monophosphate, which is applied in the field of determining the content of cyclic adenosine monophosphate in red dates, can solve the problems of complicated operation, incompleteness, long extraction time, etc., and achieves improved extraction rate, reduced concentration cost, and improved method Highly feasible effect

Inactive Publication Date: 2015-11-18
新疆农业科学院农产品贮藏加工研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current method for determining cyclic adenosine monophosphate in jujube has a long and incomplete extraction time, and the operation is cumbersome. Finding a fast and accurate method for determining cyclic adenosine monophosphate in jujube is the focus and difficulty of research in this field
Ultrasonic method is a new and effective method for extracting active components of Chinese herbal medicine (Optimization of process conditions for extraction of cyclic adenosine monophosphate from jujube by ultrasonic-assisted enzymatic method, Food Science and Technology, 2013, 7(38):220-224), supercritical carbon dioxide extraction of jujube The beneficial ingredients are also widely used, see Chinese patent, title of invention: a high-content jujube cyclic adenosine monophosphate extract and its preparation method, application number: 201310443550.6, and the jujube fruit cyclic adenosine monophosphate is extracted by ultrasonic-assisted supercritical carbon dioxide method The method and determination of its content have not been reported yet

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  • A method for determining the content of cyclic adenosine monophosphate in jujube

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Embodiment 1

[0033] A method for measuring the content of cyclic adenosine monophosphate in jujube, comprising the following steps:

[0034] (1) Extraction of cyclic adenosine monophosphate in jujube:

[0035] (a) pretreatment: put the jujube fruit with a water content of 5% into a blast drying oven at 50° C. to dry for 72 hours, then remove the core, pulverize, and pass through a 40-mesh sieve;

[0036] (b) Ultrasonic supercritical leaching: Accurately weigh 5.0000 g of dried jujube powder, put it into an ultrasonic supercritical fluid extraction kettle with an ultrasonic device, and use CO 2 Carry out low-temperature supercritical fluid extraction, then add 5% ethanol extract with a concentration of 2 times the weight of the powder, close the sealing cover of the kettle body, and pass through the CO with a purity of 99.8%. 2 Control critical temperature at 25°C, critical pressure at 3.5MPa, CO 2 Flow rate 10kg / h, extraction time 1.5h, ultrasonic time 0.5, ultrasonic power 600w, frequen...

Embodiment 2

[0049] A method for measuring the content of cyclic adenosine monophosphate in jujube, comprising the following steps:

[0050] (1) Extraction of cyclic adenosine monophosphate in jujube:

[0051] (a) pretreatment: put the jujube jujube fruit with a water content of 15% into a 50° C. blast oven for drying for 72 hours, then remove the core, pulverize, and pass through a 60-mesh sieve;

[0052] (b) Ultrasonic supercritical leaching: Accurately weigh 5.0000 g of dried jujube powder, put it into an ultrasonic supercritical fluid extraction kettle with an ultrasonic device, and use CO 2 Carry out low-temperature supercritical fluid extraction, then add 10% ethanol extract with a concentration of 5 times the weight of the powder, close the sealing cover of the kettle body, and pass through the CO with a purity of 99.8%. 2 Control critical temperature at 30°C, critical pressure at 5.5MPa, CO 2 Flow rate 10kg / h, extraction time 2.5h, ultrasonic time 1.0h, ultrasonic power: 600w, fr...

Embodiment 3

[0060] A method for measuring cyclic adenosine monophosphate content in jujube, characterized in that it comprises the following steps:

[0061] (1) Extraction of cyclic adenosine monophosphate in jujube:

[0062] (a) pretreatment: put the jujube fruit with 8% water content into a 50° C. blast drying box for drying for 72 hours, then remove the core, pulverize, and pass through a 40-60 mesh sieve;

[0063] (b) Ultrasonic supercritical leaching: Accurately weigh 5.0000 g of dried jujube powder, put it into an ultrasonic supercritical fluid extraction kettle with an ultrasonic device, and use CO 2 Carry out low-temperature supercritical fluid extraction, then add 6% ethanol extract with a concentration of 2-5 times the weight of the powder, close the sealing cover of the kettle body, and pass through the CO with a purity of 99.8%. 2 Control critical temperature at 26°C, critical pressure at 4.0MPa, CO 2 Flow rate 10kg / h, extraction time 2.0h, ultrasonic time 0.8h, ultrasonic p...

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Abstract

The invention discloses a method for measuring cyclic adenosine monophosphate content in red date. The method for measuring the cyclic adenosine monophosphate content in the red date includes steps that (1) extracting cyclic adenosine monophosphate from the red date; (2) performing high efficacy liquid chromatography. The method for measuring the cyclic adenosine monophosphate content in the red date improves the conventional cyclic adenosine monophosphate measurement method, the cyclic adenosine monophosphate extraction efficiency from the red date is improved, and the extraction rate is improved to more than 95% from the extraction rate 85% of the conventional method; the comprehensive utilization ratio of the resource is high, because the solvent dosage is greatly reduced, the cyclic adenosine monophosphate content in the leach liquor is high, the later condensation cost is greatly lowered, and the environment protection is realized; the recovery rate is 109%, the precision is high, the result is precise, and the feasibility is high.

Description

technical field [0001] The invention relates to the fields of extraction and content determination of effective plant components, in particular to a method for determining the content of cyclic adenosine monophosphate in jujube. Background technique [0002] The molecular formula of cyclic adenosine monophosphate (cyclic adenosine monophosphate, referred to as cAMP) is: C 10 h 12 N 5 o 6 P·H 2 O, the hydrate is off-white or light yellow crystal, slightly soluble in water, insoluble in ethanol or ether; melting point is 219-220°C, specific rotation is -51.3° (C=0.67); it is resistant to acid, alkali, heat All fairly stable. cAMP is an important biologically active substance in the human body. It is an intermediary factor that transmits hormones and transmitters in cells, and participates in the regulation of various physiological and biochemical processes as a second messenger. , psoriasis and other diseases have a significant effect. cAMP was first discovered from mam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 孟伊娜赵鑫周晓明过利敏苗昊翠张平张谦马燕邹淑萍张健许铭强
Owner 新疆农业科学院农产品贮藏加工研究所