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A kind of dendrobium officinale extract and its preparation method and application

A technology of Dendrobium officinale and Dendrobium officinale polysaccharide, which is applied in the field of medical and health food, can solve the problems of insufficient utilization of active ingredients, unclear material basis, single active ingredient, etc., and achieve significant pharmacological efficacy and activity, large dose-effect range, and extraction short time effect

Active Publication Date: 2017-10-17
ZHEJIANG JIGONGYUAN PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the preparation methods of Dendrobium officinale extracts reported in the literature focus on polysaccharides, and the active ingredients are single. The extraction methods mainly include heating extraction, enzymatic hydrolysis, ultrasonic-assisted extraction, microwave-assisted extraction, etc., and water is used as the extraction method. Solvent, there are disadvantages such as insufficient utilization of active ingredients and low extraction efficiency
[0004] In addition, the research on the composition of Dendrobium officinale mainly focuses on the polysaccharide content, and no in-depth research has been carried out on its composition, and other components have also been analyzed, which has disadvantages such as unclear material basis and uncontrollable quality.

Method used

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  • A kind of dendrobium officinale extract and its preparation method and application
  • A kind of dendrobium officinale extract and its preparation method and application
  • A kind of dendrobium officinale extract and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1 assay method

[0027] (1) Determination of polysaccharide content

[0028] Preparation of reference substance solution Take an appropriate amount of anhydrous glucose reference substance, accurately weigh it, add water to make a solution containing 90 μg per 1 ml, and obtain the solution.

[0029] Preparation of standard curve Accurately measure 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml of the reference substance solution, put them in 10ml stoppered test tubes respectively, add water to make up to 1.0ml, add 1ml of 5% phenol solution precisely (for immediate use) preparation), shake well, then accurately add 5ml of sulfuric acid, shake well, place in a boiling water bath and heat for 20 minutes, take it out, put it in an ice bath and cool for 5 minutes, take the corresponding reagent as a blank, according to UV-Vis spectrophotometry, at 488nm Measure the absorbance at the wavelength, draw the standard curve with the absorbance as the ordinate and the concentration...

Embodiment 2

[0046] The preparation of embodiment 2 Dendrobium officinale extract

[0047] (1) Degreasing: take 500 g of decoction pieces of Dendrobium candidum, add 5 times the amount of petroleum ether to soak for 0.5 h, then reflux twice, each time for 2 h, filter, and evaporate the organic solvent to obtain medicinal residues;

[0048] (2) Flash extraction: take the above dregs, add 5 times the amount of 30% ethanol solution to soak for 0.5h, and then perform flash extraction. The flash extraction time is 1min, and the flash extraction speed is 3500rpm, filter, and the dregs are repeatedly extracted once, and the combined filtrate is concentrated. Until there is no alcohol smell, each ml of concentrated solution is equivalent to 1.0g of crude drug;

[0049] (3) Protein removal: Take the above concentrated solution, add Sevage reagent 1 / 5 times the volume of the concentrated solution, shake vigorously for 10 minutes, let stand for 10 minutes, then centrifuge at 4000r / min for 10 minutes,...

Embodiment 3

[0051] The preparation of embodiment 3 Dendrobium officinale extract

[0052] (1) Degreasing: take 500 g of Herba Dendrobii decoction pieces, add 8 times the amount of n-hexane to soak for 1 hour, then reflux once, each time for 2 hours, filter, and evaporate the organic solvent to obtain medicinal residues;

[0053] (2) Flash extraction: take the above-mentioned dregs, add 20 times the amount of 5% ethanol solution to soak for 1h, and then carry out flash extraction, the flash extraction time is 3min, and the flash extraction speed is 4500rpm, filter, and the dregs are repeatedly extracted 2 times, and the combined filtrate is concentrated to No alcohol smell, each ml of concentrated solution is equivalent to 0.5g of crude drug;

[0054] (3) Protein removal: Take the above concentrated solution, add Sevage reagent 1 / 4 times the volume of the concentrated solution, shake vigorously for 10 minutes, let stand for 10 minutes, then centrifuge at 5000r / min for 10 minutes, divide th...

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Abstract

The invention provides a dendrobium officinale extract. In the extract, the content of dendrobium officinale polysaccharides is 70.0-90.0%, and the content of general flavones is 0.5-1.0%. The dendrobium officinale extract is capable of being used for preparing medicines or health products for preventing and treating embolic cardiovascular and cerebrovascular diseases and renal diabetes. The invention further provides a preparation method of the extract. The preparation method specifically comprises the four steps of organic solvent degreasing, flash extraction after ethanol solution soaking, Sevage reagent deproteinization and active carbon decolouration. The preparation method is capable of protecting the dendrobium officinale effective ingredient not to be thermally broken and furthest effectively extracting the dendrobium officinale polysaccharides and the flavones simultaneously, low in solvent dosage, short in extraction time, and capable of improving product quality and reducing production cost.

Description

technical field [0001] The invention belongs to the field of medicine and health food, and in particular relates to an extract of Dendrobium candidum and its preparation method and application. Background technique [0002] Dendrobium officinale Kimura.et Migo is a perennial herb of the genus Dendrobium orchidaceae. Its main medicinal parts are fresh or dried stems. It is mainly distributed in Anhui, Zhejiang, Guangxi, Hunan, Yunnan, Guizhou and other places. Chinese medicinal materials. Dendrobium candidum contains polysaccharides, flavonoids, proteins, 17 kinds of amino acids and inorganic elements, etc. It has the effects of nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat. "Shen Nong's Materia Medica" listed it as the top grade, and the Taoist health classic "Tao Zang" praised it as the first of the "Nine Chinese Immortal Grass". Folks call Dendrobium candidum "Life-saving Immortal Grass" and "Inch Golden Grass". [0003] Most of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61K36/8984A61P7/02A61P9/00A61P13/12A61P3/10A23L33/105
Inventor 王建方吴健印晓红陈琳虞利东朱明旺李立雷汤郑静曹晓铃
Owner ZHEJIANG JIGONGYUAN PHARMA
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