Serum monoamine oxidase detection kit

A monoamine oxidase and detection kit technology, which is applied in the measurement of color/spectral characteristics and analysis by chemical reaction of materials, etc., can solve the problems of poor anti-interference ability, cumbersome operation, unsuitable automatic analysis, etc., so as to improve the accuracy of detection. performance, ease of use and operation, improved stability and anti-interference ability

Inactive Publication Date: 2015-07-01
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the shortcomings of the above-mentioned technical operations such as cumbersome operation, poor anti-interference ability, and unsuitability for automatic analysis, the present invention provides a monoamine oxidase (MAO) single-reagent detection kit with simple operation, high sensitivity, strong anti-interference ability, and good stability

Method used

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  • Serum monoamine oxidase detection kit
  • Serum monoamine oxidase detection kit
  • Serum monoamine oxidase detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] In the monoamine oxidase detection kit described in this embodiment, the reagent R is composed of the following components:

[0040] HEPES buffer (pH8.5) 100mM

[0041] Benzylamine 20mM

[0042] Alpha-ketoglutarate 10mM

[0043] Reduced coenzyme 0.25mM

[0044] Trehalose 5g / L

[0045] NaCl 1g / L

[0046] Glucose 5g / L

[0047] Glucose dehydrogenase 5U / L

[0048] Glutamate dehydrogenase 3KU / L

[0049] Lauryl dimethyl betaine 1g / L

[0050] BSA 2g / L

[0051] Sodium azide 1g / L.

[0052] The method of using the monoamine oxidase detection kit in this embodiment is as follows: use a fully automatic biochemical analyzer, such as Toshiba 40 fully automatic analyzer, etc., and use the single reagent rate method for determination. Place the reagent R on the corresponding reagent position, and place the distilled water and the sample on the corresponding position of the sample plate. The operation is shown in Table 1.

[0053] Table 1 Example 1 reagent detection method

...

Embodiment 2

[0057] Interfering test

[0058] Take fresh mixed serum, divide it into 2 equal parts, and then divide each equal part into 5 equal parts, add different interfering substances, so that the concentration in the serum reaches the requirements in Table 2. Then the reagents obtained in Example 1 were used to compare and measure the content of MAO in serum at the same time as the common and recognized MAO reagents in the market. The results of the control group and the results of each group after adding different interfering substances are shown in Table 2. Relative deviation (%) = (measuring mean value of interference samples - measuring mean value of control samples) / measured mean value of control samples × 100%.

[0059] It can be seen from Table 2 that the reagent of Example 1 has no obvious interference on the test results when ascorbic acid ≤ 50 mg / dL, bilirubin ≤ 40 mg / dL, hemoglobin ≤ 200 mg / dL, ammonia ≤ 50 μmol / L. However, the reagents of other examples were significantl...

Embodiment 3

[0063] correlation experiment

[0064] The reagent was prepared using the formula of Example 1, and compared with the monoamine oxidase kit of a company approved by the State Food and Drug Administration, which is common in the market, the test was carried out. 20 clinical serum samples were tested at the same time, and the test results are shown in Table 5. And obtained the correlation curve of the two reagents (such as figure 1 Shown), the test results show that the correlation coefficient of the two kits is 0.9985, indicating that there is a great correlation between the two.

[0065] Table 3 Example 1 reagent and market common and recognized monoamine oxidase assay kit comparative detection results

[0066] .

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Abstract

The invention relates to the technical field of the clinical in-vitro detection and discloses a serum monoamine oxidase detection kit. The serum monoamine oxidase detection kit is characterized in that a reagent is a single reagent and can be used and operated conveniently compared with dual reagents on a full-automatic biochemical analysis instrument. The ultraviolet spectrophotometry is adopted to test the content of monoamine oxidase in serum; compared with the domestic common kit, the kit adopts new buffer solution and stabilizers, the stability of the reagent is improved remarkably and the detection accuracy is ensured; a coenzyme NADH (Nicotinamide Adenine Dinucleotide Hydrogen) regeneration system is adopted, a reaction substrate NADH can keep the stability all the time in a reagent storage process under the participation of enough glucose dehydrogenase, and the reagent stability and the detection accuracy are improved remarkably; novel ampholytic surfactant-dodecyl dimethyl betaine is adopted, the determination performance is improved remarkably, and the reagent stability and the anti-jamming capability are improved remarkably.

Description

technical field [0001] The invention relates to a biological detection reagent, in particular to a detection kit for clinically measuring the content of monoamine oxidase in serum, and belongs to the technical field of clinical in vitro detection. Background technique [0002] Monoamine oxidase (monoamine oxidase, MAO) is an enzyme that catalyzes the oxidative deamination of monoamine, also known as flavin-containing amine oxidase. Monoamine oxidase (MAO) has two isomers, monoamine oxidase A (MAO-A) and monoamine oxidase B (MAO-B), both of which have a flavin adenine dinucleotide covalently linked to the active center region on the cysteine ​​residue. They can catalyze and oxidize various amines in organisms: dopamine, 5-hydroxytryptamine, norepinephrine, tryptamine, etc. The final products of aldehyde and hydrogen peroxide are closely related to the oxidation of cells. Monoamine oxidase is mostly found in various organs of vertebrates, especially secretory glands, brain, ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/33G01N21/75
Inventor 甘宜梧李志明谭柏清王绮李静王春艳
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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