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Method for obtaining flavonoid aglycone from ginseng stems and leaves

A technology of ginseng stems and leaves and flavonoid aglycones, which is applied in the field of preparation of ginseng stems and leaves flavonoid aglycones, can solve the problems that have not yet been reported on the research of ginseng stems and leaves flavonoid glucosides and flavone aglycones, and achieve low cost, rich sources of raw materials, and easy operation. The effect of simple process

Active Publication Date: 2015-07-22
YANBIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no research report on the conversion of ginseng stem and leaf flavonoid glycosides into flavonoid aglycones by hydrochloric acid hydrolysis

Method used

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  • Method for obtaining flavonoid aglycone from ginseng stems and leaves
  • Method for obtaining flavonoid aglycone from ginseng stems and leaves
  • Method for obtaining flavonoid aglycone from ginseng stems and leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Weigh 1Kg of dried and crushed ginseng stems and leaves powder; add 20L of ethanol with a volume fraction of 67% and ultrasonically extract for 50 minutes, filter, concentrate, and use 3 times the volume of petroleum ether to decolorize three times to obtain a crude extract of total flavonoids from ginseng stems and leaves; add methanol to form 15mg / mL solution; put the solution into a closed hydrolysis tube, add 1 / 4 volume of 2mol / L hydrochloric acid solution, and place it in a constant temperature water bath at 60°C for 3 hours; concentrate under reduced pressure to extract; dry to obtain flavonoids from ginseng stems and leaves Aglycone, based on quercetin, kaempferol and isorhamnetin, the content of aglycone is 6.08%, and the conversion rate is 95.89%.

Embodiment 2

[0051] Weigh 1Kg of dried and crushed ginseng stems and leaves powder; add 20L of 67% ethanol for ultrasonic extraction for 50 minutes, filter and concentrate, use 3 times the volume of petroleum ether to decolorize three times to obtain the crude extract of total flavonoids of ginseng stems and leaves; add methanol to prepare 15 mg / mL solution; put the solution into a closed hydrolysis tube, add 1 / 4 volume of 4mol / L hydrochloric acid solution, and place it in a constant temperature water bath at 50°C for 3 hours; concentrate under reduced pressure to obtain an extract; dry it to obtain flavonoids from ginseng stems and leaves Aglycone, based on quercetin, kaempferol and isorhamnetin, the content of aglycone is 5.54%, and the conversion rate is 95.48%.

Embodiment 3

[0053] Weigh 1Kg of dried and crushed ginseng stems and leaves powder; add 20L of 67% ethanol for ultrasonic extraction for 50 minutes, filter and concentrate, use 3 times the volume of petroleum ether to decolorize three times to obtain the crude extract of total flavonoids of ginseng stems and leaves; add methanol to prepare 15 mg / mL solution; put the solution into a closed hydrolysis tube, add 1 / 4 volume of 4mol / L hydrochloric acid solution, and place it in a constant temperature water bath at 70°C for 4 hours; concentrate under reduced pressure to extract; dry to obtain flavonoids from ginseng stems and leaves Aglycone, based on quercetin, kaempferol and isorhamnetin, the aglycon content is 5.58%, and the conversion rate is 95.53%.

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Abstract

The invention relates to a method for preparing flavonoid aglycone from ginseng stems and leaves. The method comprises the following steps: extracting a crude extract of total flavonoids of ginseng stems and leaves through an ultrasonic technology; preparing the solution of the crude extract of the total flavonoids of the ginseng stems and leaves; converting flavone glycoside in the solution of the crude extract of the total flavonoids of the ginseng stems and leaves into flavonoid aglycone; drying. According to the method for obtaining the flavonoid aglycone from the ginseng stems and leaves provided by the invention, the operation process is simple; the time is short; the cost is low; the conversion rate is low; the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method of ginseng stem and leaf flavonoid aglycone, specifically, a method for obtaining flavonoid aglycone from ginseng stem and leaf by hydrolysis with hydrochloric acid. The invention relates to a preparation method of active ingredients of traditional Chinese medicine, in particular to a method for preparing flavonoid aglycone from ginseng stems and leaves. Background technique [0002] Ginseng (Panax ginseng C.A.Meyer) is the root of the plant Panax of the Araliaceae family (Araliaceae), and is a valuable traditional Chinese medicine. It is used for physical weakness, cold limbs, slight pulse, lung deficiency, wheezing and coughing, palpitation, insomnia, neurasthenia, mental fatigue and various insufficiency of Qi, blood and body fluid. The medicinal part of ginseng recorded in the ancient medical book "Shen Nong's Materia Medica" is mainly the underground part, that is, the roots and whiskers of ginseng. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61K31/352A61K135/00A61K127/00
Inventor 崔福顺李官浩
Owner YANBIAN UNIV
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