Colloidal gold for campylobacter jejuni antigen detection, glass fiber sample pad containing colloidal gold, detection reagent strip and detection kit

Abstract

The invention belongs to the technical field of biological detection and in particular relates to colloidal gold for campylobacter jejuni antigen detection, a glass fiber sample pad containing the colloidal gold, a detection reagent strip and a detection kit. The colloidal gold is prepared by virtue of a sodium citrate reduction method, and a polyvinylpyrrolidone stabilizer is added in the colloidal gold preparation process. The glass fiber sample pad comprises a colloidal gold-antibody conjugate and glass fibers; the campylobacter jejuni antigen detection reagent strip comprises a supporting plate, an antibody-nitrocellulose membrane, an absorbent pad, the glass fiber sample pad and a glass fiber sample adding pad; and the campylobacter jejuni antigen detection kit comprises a detection card and a detection strip. The colloidal gold for campylobacter jejuni antigen detection provided by the invention is uniform in particle size and high in dispersity and stability; and the campylobacter jejuni antigen detection reagent strip and the detection kit are fast, uniform and stable in color developing, high in sensitivity, convenient to use and easy to operate, and an organic unification of a complicated principle and simple operation is really realized.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a colloidal gold used for detecting Campylobacter jejuni antigen, a glass fiber sample pad containing colloidal gold, a detection reagent strip and a detection kit. Background technique [0002] Campylobacter jejuni (Campylobacter jejuni) is one of the important zoonotic pathogens affecting animal-derived food safety in recent years. It is listed as the three major human diarrhea pathogens together with Salmonella and Shigella. Tops the list in bacterial diarrheal diseases. Campylobacter jejuni infection is mostly sporadic, and outbreaks are rare. It is the main pathogen of diarrheal diseases in summer and autumn, especially among preschool children. The bacterium is widespread in poultry intestines and can cause infection in humans by contaminating meat, food or water. [0003] In my country, Campylobacter jejuni was first isolated from the excrement o...

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Problems solved by technology

[0004] At present, there are many inconveniences and deficiencies in the routine detection methods of Campylobacter jejuni used in China, such as harsh culture and isolation conditions, difficulty in separating pure colonies, long detection cycle, poor repeatability of biochemical identification results, etc., resulting in the detection of Campylobacter jejuni. Reporting rates have been low (6 to 9%)

[0005] In recent years, with the development of colloidal gold immunochromatography technology, detection reagents using colloidal gold method to detect Campylobacter jejuni antigens have appeared. Colloidal gold immunochromatography technology refers to immobilizing specific antigens or antibodies in strips On the membrane, the colloidal gold labeling reagent (antibody or monoclonal antibody) is adsorbed on the binding pad. When the sample to be tested is added to the sample pad at one end of the test strip, it moves forward through capillary action and dissolves the colloidal gold labeling reagent on the binding pad. Mutual reaction, when moving to the area of ​​fixed antigen or antibody, the conjugate of the substance to be tested and the gold-labeled reagent will specifically bind to it and be intercepted, and gather on the detection zone, and the color development result can be observed by naked eyes , the sensitivity of the existing Campylobacter jejuni antigen detection reagent is 1×10 6 Bacteria / mL, for 1×10 6 The detection of Campylobacter jejuni below bacteria / mL is not sensitive enough

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