Colloidal gold for campylobacter jejuni antigen detection, glass fiber sample pad containing colloidal gold, detection reagent strip and detection kit

A technology for the detection of Campylobacter jejuni and antigens, which is applied in the field of detection kits, colloidal gold for detection of Campylobacter jejuni antigens, and glass fiber sample pads. Solve problems such as harsh separation conditions, achieve good uniformity, ensure food safety, and be less likely to be contaminated

Inactive Publication Date: 2015-07-22
山东康华生物医疗科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many inconveniences and deficiencies in the routine detection methods of Campylobacter jejuni used in China, such as harsh culture and isolation conditions, difficulty in separating pure colonies, long detection cycle, poor repeatability of biochemical identification results, etc., resulting in the detection of Campylobacter jejuni. Reporting rates have been low (6 to 9%)
[0005] In recent years, with the development of colloidal gold immunochromatography technology, detection reagents using colloidal gold method to detect Campylobacter jejuni antigens have appeared. Colloidal gold immunochromatography technology refers to immobilizing specific antigens or antibodies in strips On the membrane, the colloidal gold labeling r

Method used

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  • Colloidal gold for campylobacter jejuni antigen detection, glass fiber sample pad containing colloidal gold, detection reagent strip and detection kit
  • Colloidal gold for campylobacter jejuni antigen detection, glass fiber sample pad containing colloidal gold, detection reagent strip and detection kit
  • Colloidal gold for campylobacter jejuni antigen detection, glass fiber sample pad containing colloidal gold, detection reagent strip and detection kit

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Colloidal gold used for Campylobacter jejuni antigen detection, the colloidal gold is prepared by trisodium citrate reduction method, including:

[0051] (1) Take 2mL concentration as 1.0×10 4 g / mL chloroauric acid aqueous solution, dilute to 20mL with distilled water, heat to 80°C under stirring, add 0.1mL trisodium citrate with a mass fraction of 1% after 3-5 minutes, continue heating for 10-15 minutes, cool to room temperature, get gold solution;

[0052] (2) Take 30mL concentration as 1.0×10 4 g / mL chloroauric acid and 1 mL of the gold solution prepared in the above step (1), mix, heat to boiling under stirring, add 0.1 g of polyvinylpyrrolidone stabilizer, and quickly add the strong reducing agent potassium borohydride 0.6 after 5-8 minutes mL, react under boiling for 1-3 minutes, and cool naturally to room temperature, that is.

Embodiment 2

[0054] figure 1 A schematic structural view of the glass fiber sample pad provided by the present invention is shown, and for the convenience of description, this figure only provides the structural parts related to the present invention.

[0055] like figure 1 As shown, the glass fiber sample pad 1 includes colloidal gold-antibody conjugate 11 and glass fiber 12, and the glass fiber sample pad is made by the following method:

[0056] (1) preparation of colloidal gold-antibody conjugate, described preparation method comprises the following steps:

[0057] S1 Take 100ml of the above colloidal gold, adjust the pH to 8.0, add 1.5mg of anti-Campylobacter jejuni monoclonal antibody, then add 2mL of 25mg / mL polyethylene glycol dropwise, centrifuge, discard the supernatant, and obtain a precipitate;

[0058] S2 Wash the precipitate prepared in step S1 twice with 10 mL of phosphate buffer containing 0.4 mg / mL polyethylene glycol, and wash the precipitate with 2% bovine serum albumi...

Embodiment 3

[0061] figure 2 A schematic structural view of the Campylobacter jejuni antigen detection reagent strip provided by the present invention is shown. For the convenience of description, this figure only provides the structural parts related to the present invention.

[0062] like figure 2 As shown, the Campylobacter jejuni antigen detection reagent strip includes a support plate 2 on which an antibody-nitrocellulose membrane 3 is arranged; a water-absorbing pad 4 is pasted on the antibody-nitrocellulose membrane 3, and the water-absorbing pad 4 is downward Cover the edge of the antibody-nitrocellulose membrane 3 by 0.5-1.5 mm; the above-mentioned glass fiber sample pad 1 is pasted under the antibody-nitrocellulose membrane 3, and the bottom of the glass fiber sample pad 1 is pasted with a glass fiber sample pad 5, a glass fiber The sample pad 5 upwardly covers the edge of the glass fiber sample pad 1 by 0.5-1.5 mm.

[0063] Wherein, the antibody-nitrocellulose membrane 3 is ...

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Abstract

The invention belongs to the technical field of biological detection and in particular relates to colloidal gold for campylobacter jejuni antigen detection, a glass fiber sample pad containing the colloidal gold, a detection reagent strip and a detection kit. The colloidal gold is prepared by virtue of a sodium citrate reduction method, and a polyvinylpyrrolidone stabilizer is added in the colloidal gold preparation process. The glass fiber sample pad comprises a colloidal gold-antibody conjugate and glass fibers; the campylobacter jejuni antigen detection reagent strip comprises a supporting plate, an antibody-nitrocellulose membrane, an absorbent pad, the glass fiber sample pad and a glass fiber sample adding pad; and the campylobacter jejuni antigen detection kit comprises a detection card and a detection strip. The colloidal gold for campylobacter jejuni antigen detection provided by the invention is uniform in particle size and high in dispersity and stability; and the campylobacter jejuni antigen detection reagent strip and the detection kit are fast, uniform and stable in color developing, high in sensitivity, convenient to use and easy to operate, and an organic unification of a complicated principle and simple operation is really realized.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a colloidal gold used for detecting Campylobacter jejuni antigen, a glass fiber sample pad containing colloidal gold, a detection reagent strip and a detection kit. Background technique [0002] Campylobacter jejuni (Campylobacter jejuni) is one of the important zoonotic pathogens affecting animal-derived food safety in recent years. It is listed as the three major human diarrhea pathogens together with Salmonella and Shigella. Tops the list in bacterial diarrheal diseases. Campylobacter jejuni infection is mostly sporadic, and outbreaks are rare. It is the main pathogen of diarrheal diseases in summer and autumn, especially among preschool children. The bacterium is widespread in poultry intestines and can cause infection in humans by contaminating meat, food or water. [0003] In my country, Campylobacter jejuni was first isolated from the excrement o...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 杨致亭王增祺陈利军王明儒隋振国
Owner 山东康华生物医疗科技股份有限公司
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