Expression method, special expression vector, engineering bacteria and application for recombinant human insulin

A recombinant human insulin and expression vector technology, applied in the biological field, can solve the problems of not being able to have a selection marker-resistance gene, disrupting the balance of the intestinal micro-ecosystem, and adversely affecting normal flora
CN104805091AActive Publication Date: 2015-07-29WUHAN ZHENFU PHARMA CO LTD
4 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
WUHAN ZHENFU PHARMA CO LTD
Publication Date
2015-07-29

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides an expression method, a special expression vector, engineering bacteria and an application for recombinant human insulin. According to the expression method, recombinant human insulin genes can be subjected to induced expression in food-grade lactobacillus, and the recombinant human insulin is presented on the surface of the lactobacillus. The engineering bacteria, the expression vector and an inducer, which are used in the expression method, all meet food-grade requirements, so that the potential hazard caused by existence of resistance genes of the expression vector and non-food-grade inducers is avoided. Lactobacillus engineering bacteria containing cell-wall presentation insulin obtained according to the invention can stimulate an NOD (Non-obese diabetic) rat to produce specific antibodies for the recombinant human insulin, so that the level of a cell factor IL-4 related to immune tolerance is obviously increased, and the generation of immune tolerance is induced; a microbial agent can be taken as an oral vaccine for type I diabetes mellitus as long as the induced lactobacillus engineering bacteria are prepared into the microbial agent, complicated post-processing processes, such as purification, are unnecessary, and wide application prospects are obtained.
Need to check novelty before this filing date? Find Prior Art

Description

【Technical field】

[0001] The invention relates to the field of biotechnology, in particular to a method for expressing recombinant human insulin, a special expression vector, engineering bacteria and its application. 【Background technique】

[0002] Diabetes is a chronic disease caused by insulin deficiency or deficiency caused by congenital or acquired factors. This lack of insulin leads to elevated blood sugar levels which can lead to damage to the body, especially blood vessels and nerves. Polydipsia, polyuria, polyphagia, and weight loss are typical symptoms of diabetes. In severe cases, ketone poisoning and chronic diseases of various organs may also occur.

[0003] There are two main types of diabetes: type I and type II. Type I diabetes (Type I diabetes mellitus, T1DM) is an autoimmune disease. The pancreas of the patient is destroyed by the immune system, resulting in an absolute lack of insulin. Blood sugar is stable. Type II diabetes (Type II diabetes mellitus, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More