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Preparation method of trametes versicolor fermentation liquor and application of trametes versicolor fermentation liquor in degrading aflatoxin B1

A technology for Trametes versicolor and fermentation broth, which is applied in the field of microbial fermentation, can solve the problems of not having products yet and putting them on the market, and achieves the effects of mild conditions, fast degradation speed and simple use.

Inactive Publication Date: 2015-08-12
河北省微生物研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, laccases degrade aflatoxin B 1 Studies have reported that the degradation rate is high, but no products have been put on the market

Method used

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  • Preparation method of trametes versicolor fermentation liquor and application of trametes versicolor fermentation liquor in degrading aflatoxin B1
  • Preparation method of trametes versicolor fermentation liquor and application of trametes versicolor fermentation liquor in degrading aflatoxin B1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation method of the fermented broth of Trametes versicolor is to inoculate Trametes versicolor CICC14001 in an aseptic medium containing carbon source, nitrogen source, inorganic salts and trace elements for aerobic fermentation to make a fermented liquid;

[0037] Fermentation medium is made up of the component of following mass percentage:

[0038] Bagasse 1.9%, Bran 0.12%, Bean Cake Powder 0.1%, Glucose 0.7%, Fructose 0.5%, Ammonium Sulfate 0.1%, Potassium Dihydrogen Phosphate 0.15%, Magnesium Sulfate 0.05%, Vitamin B 1 0.00003%, guaiacol 0.00013%, the volume ratio of the trace element solution to the sterile medium is 0.07%, the balance is sterile water, pH=4.5;

[0039] The technological conditions of aerated fermentation are as follows:

[0040] The inoculum volume ratio is 8:100, the temperature is 31°C, the ventilation volume is 1:0.7vvm, the pressure is 0.04Mpa, and the culture period is not less than 220 hours.

[0041] In addition to the time fact...

Embodiment 2

[0054] The difference between this embodiment and embodiment 1 is:

[0055] Fermentation medium is made up of the component of following mass percentage:

[0056] Bagasse 2.1%, Bran 0.11%, Bean Cake Powder 0.11%, Glucose 0.5%, Fructose 0.7%, Ammonium Sulfate 0.08%, Potassium Dihydrogen Phosphate 0.17%, Magnesium Sulfate 0.04%, Vitamin B 1 0.00002%, guaiacol 0.00013%, the volume ratio of the trace element solution to the sterile medium is 0.09%, the balance is sterile water, pH=4.5;

[0057] The inoculum volume ratio is 7:100, the temperature is 29°C, the ventilation volume is 1:0.9vvm, the pressure is 0.03Mpa, and the culture period is not less than 220 hours.

[0058] The culture temperature in the preparation of the slant strain is 29° C., and the culture period is 5 days.

[0059] In the preparation of described secondary seed:

[0060] The filling capacity is volume ratio = 60:100, under the conditions of temperature at 30°C, ventilation rate at 1:0.5vvm, and pressure a...

Embodiment 3

[0064] The difference between this embodiment and embodiment 1 is:

[0065] Fermentation medium is made up of the component of following mass percentage:

[0066] Bagasse 2.3%, Bran 0.10%, Bean Cake Powder 0.13%, Glucose 0.6%, Fructose 0.6%, Ammonium Sulfate 0.11%, Potassium Dihydrogen Phosphate 0.2%, Magnesium Sulfate 0.06%, Vitamin B 1 0.00001%, guaiacol 0.00013%, the volume ratio of the trace element solution to the sterile medium is 0.12%, the balance is sterile water, pH=4.5;

[0067] The inoculum volume ratio is 6:100, the temperature is 30°C, the ventilation volume is 1:1.2vvm, the pressure is 0.05Mpa, and the culture period is not less than 220 hours.

[0068] The culture temperature in the preparation of the slant strain is 31° C., and the culture period is 4.5 days.

[0069] The preparation of described secondary liquid seed comprises the following process steps:

[0070] According to the volume of the primary seed: the volume ratio of the secondary seed medium=10...

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Abstract

The invention relates to microbial fermentation and in particular relates to a preparation method of trametes versicolor fermentation liquor and the application of the trametes versicolor fermentation liquor in degrading aflatoxin B1. The trametes versicolor PTrametes versicolor CICC 14001 is inoculated to a sterile culture medium containing a carbon source, a nitrogen source, inorganic salts and trace elements and fermented into the fermentation liquor under the condition of aeration; the trametes versicolor fermentation liquor prepared by use of the method can be applied to degrading the aflatoxin B1. The preparation method is used for solving the technical problems of the existing method, such as demand on specific conditions and materials, high labor intensity, incomplete detoxification, great damage to treated materials, demand on drastic conditions, secondary pollution always caused by generation of chemical residues, and has the advantages of simple and convenient process and high safety; besides, the trametes versicolor fermentation liquor is used for degrading the aflatoxin B1, and the degradation speed is high and the degradation rate is high.

Description

technical field [0001] The present invention relates to microbial fermentation, in particular to a preparation method of Trametes versicolor fermentation liquid and the ability of Trametes versicolor fermentation liquid to degrade aflatoxin B 1 in the application. Background technique [0002] Aflatoxin (AFT) is a class of highly toxic microbial metabolites that are extremely harmful to humans and animals, especially the damage to the liver tissue of humans and animals, and can lead to liver cancer and even death in severe cases. Aflatoxins are mainly produced by Aspergillus flavus and Aspergillus parasitica, and some Aspergillus, Penicillium, Mucor, Fusarium, Rhizopus, Streptomyces and actinomycetes can also produce the toxin. Aflatoxins are widely distributed in nature, and are more common in peanuts, corn, soybeans, grains and their products. After aflatoxin enters the body, the amount in the liver is higher than that in other tissues and organs, indicating that the liv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A62D3/02C12R1/645
Inventor 王云鹏胡常英秦艳梅马清河胡科峰章淑艳李军李宾蔡建成刘丽娜韩韬罗同阳郑翔高庆华刘春卯李领颇吴芳彤
Owner 河北省微生物研究所有限公司
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