Method for culturing nitrogen fixation spirulina by using kitchen wastewater

A technology for kitchen wastewater and spirulina, which is applied in the fields of environmental engineering and microalgae cultivation, can solve problems such as unsustainable development, and achieve the effects of reducing production costs, reducing use, and simple preparation methods.

Inactive Publication Date: 2015-08-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Growing microalgae with chemicals

Method used

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  • Method for culturing nitrogen fixation spirulina by using kitchen wastewater
  • Method for culturing nitrogen fixation spirulina by using kitchen wastewater
  • Method for culturing nitrogen fixation spirulina by using kitchen wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the seed preservation culture of spirulina

[0031]The spirulina Spirulina platensis used in this example was purchased from the Freshwater Algae Species Bank of the Institute of Hydrobiology, Chinese Academy of Sciences, numbered FACHB-901. Before the reactor wastewater culture, the seed preservation culture of Spirulina platensis was carried out: the seed preservation culture was carried out in a 1L glass Erlenmeyer flask containing 600mL of sterilized culture solution, and the medium was the improved Zarrouk medium. See Tables 1 and 2 for ingredients, configuration and usage (add 2mL of A5 solution per L of culture medium). The incubator was set with a light of 3000Lux, a light-to-dark ratio of 16 / 8, and the temperature of the incubator was controlled at 30±1°C. The conservation culture lasts for 8 to 10 days.

[0032] Table 1 Components of the modified Zarrouk medium

[0033]

[0034] Table 2 Trace element stock solution A5

[0035]

Embodiment 2

[0036] Embodiment 2: Utilize the microalgae culture medium prepared by kitchen waste water to cultivate spirulina

[0037] Inoculate the algal liquid in the logarithmic phase into the photobioreactor (such as figure 1 , figure 2 shown).

[0038] Trace elements (same as the type of trace elements in the trace element stock solution A5 of Zarrouk medium) were added to kitchen waste water to prepare microalgae culture medium, and batch culture of Spirulina platensis was carried out. Microalgae culture can replace nitrogen and phosphorus chemical reagents (NaNO 3 and K 2 HPO 4 ·3H 2 0) As the source of nitrogen and phosphorus required for the growth of Spirulinaplatensis. The culture conditions were as follows: CO was fed intermittently at a rate of 0.22vvm 2 , the light is 3000Lux, the light-to-dark ratio is 16 / 8, and the temperature of the incubator is controlled at 30±1°C.

[0039] The culture medium prepared by diluting different multiples of kitchen wastewater (TN co...

Embodiment 3

[0041] Example 3: Changes of N and P elements in the culture medium during microalgae cultivation

[0042] Use tap water to dilute unsterilized kitchen wastewater until the TN concentration is about 170mg / L, add the trace element solution in Table 2 to make a microalgae culture medium, and conduct a pilot test in a 10L reactor to cultivate carbon-fixing helix algae.

[0043] During periodic operation, 50mL samples were taken multiple times to determine the content of chlorophyll a, and 50mL algae liquid was filtered with a 0.45μm filter membrane to determine the TN and NO contained in it. 3 - -N, NO 2 - -N, NH 4 + -N, TP concentration. After the sampling, the pre-diluted kitchen waste water was added back. Inject CO intermittently during cycle operation 2 , as a carbon source to provide Spirulina with carbon elements necessary for photosynthesis.

[0044] The result is as Figure 4 As shown, the OD of Spirulina 560 Enter logarithmic growth phase after 48h lag period...

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Abstract

The invention discloses a method for culturing nitrogen fixation spirulina by using kitchen wastewater, and belongs to the technical fields of environment engineering and microalgae culture. According to the method, microelements are added into kitchen wastewater so as to prepare a microalgae culture medium for culturing microalgae, an effect of a Zarrouk culture medium can be generally achieved, use of chemical medicines is reduced, the environment pollution is also reduced, meanwhile the production cost of microalgae is lowered, sustainable microalgae production can be achieved, the culture medium is simple in preparation method, the kitchen wastewater can be used for culturing the microalgae without being sterilized, resource utilization of kitchen wastewater can be achieved, environment pollution can be reduced, the utilization rate of ammonia nitrogen in the kitchen wastewater can be 100%, the utilization rate of nitrous nitrogen can be up to 72.361%, the utilization rate of nitrate nitrogen is 7.849%, the utilization rate of TN is 32.035%, the absorption property of carbon sequestration spirulina on the nitrogen element is that NO2<-> -N is less than NH4<+>-N and greater than NO3<->-N, the nitrogen source in the wastewater is sufficient for spirulina to grow, and the utilization rate of TP is 59.140%.

Description

technical field [0001] The invention relates to a method for cultivating nitrogen-fixing spirulina by using kitchen wastewater, and belongs to the technical fields of environmental engineering and microalgae cultivation. Background technique [0002] With the combustion of fossil fuels and vehicle exhaust emissions, resulting in CO 2 A series of environmental problems that threaten the survival and development of human beings, such as the sharp increase of greenhouse gases and the intensification of the global greenhouse effect. Therefore, the focus of global attention is on reducing CO 2 emissions, the current focus on reducing CO 2 The technology mainly includes physical, chemical and biological methods, but because microalgae in CO 2 In terms of emission reduction, CO can be fixed through photosynthesis 2 The synthesis of organic compounds has the characteristics of fast growth, short cycle and high efficiency. However, most studies have shown that the production of m...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/20
Inventor 任洪艳徐倩阮文权
Owner JIANGNAN UNIV
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