Composite microbial culture starter and preparation method thereof

A technology of compound microorganisms and starters, applied in the preparation of organic fertilizers, treatment of biological organic parts, fixation on or in inorganic carriers, etc., can solve problems such as low fertilizer efficiency, few functions, and physical disadvantages of agricultural practitioners, and achieve Improve resource attributes, solve pollution, and improve the effect of soil

Inactive Publication Date: 2015-08-26
陆少英
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Problems solved by technology

The nutrients contained in the above organic wastes have the value of recycling. After inoculation, fermentation and composting, they can be made into organic fertilizers, which can be used as nutrients for plant growth, improve soil structure, increase soil fertility, and promote crop growth. However, in the existing technology, the above-mentioned different types of organic wastes are often fermented separately and then formulated into organic fertilizers, which not only has few functions, low fertilizer efficiency, and low comprehensive utilization rate of organic wastes, but also cannot effectively kill harmful pathogenic bacteria, which is very smelly , unfavorable to the health of agricultural practitioners

Method used

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Examples

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Effect test

preparation example Construction

[0026] The preparation method of composite microorganism starter of the present invention may further comprise the steps:

[0027]1. Inoculation, according to the temperature and humidity range of the clean room and the cleanliness level of suspended particles in the air stipulated in the standard "Code for Design of Clean Plants" GB50073-2001, respectively Bacillus subtilis, Lactobacillus acidophilus, Lactobacillus delbrueckii Subspecies, Saccharomyces cerevisiae, Bacillus megaterium and Clostridium pasteuriani were respectively inoculated on slant surfaces according to the prior art, at room temperature (20°C), relative humidity was 50%, cultivated for 16-20h, and then each was added to sterile water After shaking for 30 minutes, the bacterial liquid was obtained, counted with a hemocytometer, and the number of bacteria in the bacterial liquid reached 200-300 million / ml, preferably 200 million / ml.

[0028] Two, one-level cultivation (one-level expansion cultivation), the bac...

Embodiment 1

[0043] 1. Inoculation: Inoculate Bacillus subtilis, Lactobacillus acidophilus, Lactobacillus delbrueckii subspecies bulgaricus, Saccharomyces cerevisiae, Bacillus megaterium and Clostridium pasteurianus respectively, at room temperature, humidity is 50%, cultivate 18h, then Each was added into sterile water, shaken for 30 minutes to obtain a bacterial liquid, and the number of bacteria in the bacterial liquid reached 200 million / ml respectively.

[0044] Two, first-level culture, inoculate the strains of each bacterium solution respectively in a 50ml conical flask filled with a first-level liquid medium, the inoculum size is 5% v / v, the pH value of the medium is 6.00, and the conical flask Put it into a shaking table and shake it at 180r / min for 32h, and the number of bacteria in the bacterial solution after the first-level expansion reaches 200 million / ml.

[0045] 3. Secondary culture, mix each bacterial solution of the first-level expansion culture with the second-level liq...

Embodiment 2

[0048] 1. Inoculation: Bacillus subtilis, Lactobacillus acidophilus, Lactobacillus delbrueckii subsp. bulgaricus, Saccharomyces cerevisiae, Bacillus megaterium and Clostridium pasteurianus were inoculated on a slant, at room temperature, the humidity was 50%, cultivated for 18 hours, Then each was added into sterile water, shaken for 30 minutes to obtain a bacterial liquid, and the number of bacteria in the bacterial liquid reached 200 million / ml respectively.

[0049] Two, primary culture, inoculate the bacterial classification of each bacterial liquid described in the 50ml Erlenmeyer flask that fills primary liquid culture medium respectively, inoculum size 8%v / v, the pH value of culture medium is 6.00, Erlenmeyer flask Put it into a shaking table and shake it at 180r / min for 32h, and the number of bacteria in the bacterial solution after the first-level expansion reaches 200 million / ml.

[0050] 3. Secondary culture, mix each bacterial solution of the first-level expansion ...

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Abstract

The invention discloses a composite microbial culture starter and a preparation method thereof, and aims to solve the technical problem of efficient and comprehensive organic waste fermentation and composting processing. The composite microbial culture starter comprises a carrier and a compound strain, the carrier is natural zeolite powder, and the compound strain comprises bacillus subtilis, lactobacillus acidophilus, lactobacillus delbrueckii subspecies bulgaricus, saccharomyces cerevisiae, bacillus megatherium and clostridium pasteurianum. The preparation method includes inoculation, primary culture, secondary culture, mixing to obtain a compound bacterium fluid, adsorption of the compound microbial into the natural zeolite powder with the granularity of 200 mesh, and drying to obtain the composite microbial culture starter. Compared with the prior art, by efficient and comprehensive of the decompose organic wastes, the pollution caused by the organic wastes on the environment can be completely solved by the composite microbial culture starter, the resource properties of the organic wastes are improved, and the prepared multifunctional organic fertilizer provides comprehensive nutrients needed for plant growth, and improves the soil very well.

Description

technical field [0001] The invention relates to an organic fertilizer and a preparation method thereof, in particular to a microbial fermentation agent of an organic fertilizer and a preparation method thereof. Background technique [0002] According to the current two-way development model of agricultural industrialization and urbanization in cities and towns, the amount of organic solid waste produced is increasing day by day. From the perspective of agricultural industrial structure, the main source of agricultural waste comes from animal husbandry and planting. On the other hand, urban household garbage contains a large amount of organic waste, such as kitchen waste and sludge produced every day by sewage treatment plants. The organic waste produced by animal husbandry and planting has the value of recycling. Among them, poultry manure contains a large number of elements such as nitrogen, phosphorus, and potassium needed for crop growth, and also contains calcium, magnes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C05F17/00C05F11/08
CPCY02W30/40
Inventor 陆少英
Owner 陆少英
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