Method for preparing high-purity sulforaphene from carmine radish seeds

Abstract

The invention discloses a method for preparing high-purity sulforaphene from carmine radish seeds, and relates to a method and a technology for rapidly and steadily preparing sulforaphene from the carmine radish seeds. According to the method, the powder of skimmed carmine radish seeds sold in the market is taken as a raw material; through enzymolysis and centrifugation, a centrifugate is obtained; resin coarse separation and high performance liquid chromatography preparation separation are sequentially conducted on the obtained centrifugate, so that a sulforaphene preparation liquid is obtained; finally, through rapid alcohol-water displacement, concentration, and freeze-drying, a sulforaphene product of which the purity reaches 99% or higher is obtained. The method is simple to operate, efficient, and high in continuity, and has the advantages that solvents can be repeatedly recovered and utilized, resin can be recycled, the sulforaphene product is steady and high in purity, and the problems that sulforaphene is liable to be subjected to rearrangement or isomerization in water, and the sulforaphene product is difficult to preserve for a long time can be solved. The sulforaphene product prepared according to the method can be widely applied to the fields of health products and pharmaceuticals.

Description

1. Technical field [0001] This patent belongs to the field of preparation and stability research of radish, and in particular relates to a method for rapidly and stably preparing high-purity radish from rouge radish seeds. 2. Background technology [0002] Rathotin is a class of isothiocyanate substances obtained by hydrolyzing glucosinolates in cruciferous vegetables in myrosinase, and its scientific name is 1-isothiocyanate-4-methylsulfonyl-butane. Also known as sulforaphane, sulforaphane, soluble in water, methanol and chloroform, easily soluble in ether, insoluble in petroleum ether. Rathuanin is one of the strongest anti-cancer ingredients found in vegetables so far. It can induce the body to produce type II detoxification enzymes, prevent carcinogens from destroying the genetic factors of healthy cells, and has broad market prospects. [0003] Existing technology for preparing radishine, for example, the application number is 201110117510.3, and the title is "a kind o...

AI Technical Summary

Problems solved by technology

The main problem that said method exists is: 1. select organic solvents such as dichloromethane, ethyl acetate to extract radishone, and this type of solvent is highly toxic, volatile or highly water-soluble, and pollutes the environment; The target object, the time is long and the adsorption efficiency is low

Since radishene begins to rearrange or isomerize within 30 minutes in aqueous solution, long-term static adsorption will lead to a large amount of rearrangement or isomerization of sulforaphane cyanide or isoxane and other impurities, which will significantly reduce the yield of radishene efficiency and increase the difficulty of separation and purification; ③ the above method only uses extraction and adsorption for purification, because the selectivity of extraction and adsorption to the target substance is low, the purity of the prepared radish is not high, and it can only be used in the food field. low value