Method for preparing high-purity sulforaphene from carmine radish seeds

A high-purity technology of rapin, applied in the field of preparation and stability research of rapin, can solve the problems of increasing the difficulty of separation and purification, high volatility or high water solubility, and reducing the yield of rapin, and achieves green environmental protection performance. The effect of low consumption, simple installation and low cost

Active Publication Date: 2015-09-02
GUANGZHOU LIUSHUN BIO TEC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem that said method exists is: 1. select organic solvents such as dichloromethane, ethyl acetate to extract radishone, and this type of solvent is highly toxic, volatile or highly water-soluble, and pollutes the environment; The target object, the time is long and the adsorption efficiency is low
Since radishene begins to rearrange or isomerize within 30 minutes in aqueous solution, long-term static adsorption will lead to a large amount of rearran

Method used

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Examples

Experimental program
Comparison scheme
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Example Embodiment

[0021] Example 1

[0022] A method for preparing high-purity lanolin from carmine radish seeds, the specific steps are as follows:

[0023] (1) Preparation of crude extract of raisin

[0024] First, prepare a citric acid-trisodium citrate buffer solution with a pH of 3.0 and a concentration of 0.1 mol / L or a citric acid buffer solution with a pH of 3.0 and a concentration of 0.1 mol / L, and then follow the commercial defatted radish seeds The ratio of the mass of powder (kg) to the volume (L) of the buffer solution is 1:4. Under stirring, disperse the defatted carmine radish seed powder on the market in the buffer solution, and hydrolyze the solution at a constant temperature of 30°C for 1.5 hours. . After the hydrolysis reaction is completed, filter, collect the filtrate and the filter residue, and use the collected filter residue as a livestock feed additive after drying; for the collected filtrate, adjust the pH to 2 with a dilute HCl solution and then use a tubular centrifuge at...

Example Embodiment

[0031] Example 2

[0032] A method for preparing high-purity lanolin from carmine radish seeds, the specific steps are as follows:

[0033] (1) Preparation of crude extract of raisin

[0034] First, prepare a citric acid-trisodium citrate buffer solution with a pH of 4.5 and a concentration of 0.5mol / L or a citric acid buffer solution with a pH of 4.5 and a concentration of 0.5mol / L, and then follow the commercially available defatted radish seeds The ratio of the mass of powder (kg) to the volume (L) of the buffer solution is 1:10, and the commercially available defatted radish seed powder is dispersed in the buffer solution under stirring, and hydrolyzed at a constant temperature of 40° C. for 3 hours. After the hydrolysis reaction is completed, filter, collect the filtrate and the filter residue, and use the collected filter residue as a livestock feed additive after drying; for the collected filtrate, adjust the pH to 4 with a dilute HCl solution and then use a tubular centrifug...

Example Embodiment

[0041] Example 3

[0042] A method for preparing high-purity lanolin from carmine radish seeds, the specific steps are as follows:

[0043] (1) Preparation of crude extract of raisin

[0044] First, prepare a citric acid-trisodium citrate buffer solution with a pH of 4.0 and a concentration of 0.3 mol / L or a citric acid buffer solution with a pH of 4.0 and a concentration of 0.3 mol / L, and then follow the commercially available defatted radish seeds The ratio of the powder mass (kg) to the buffer solution volume (L) is 1:7, and the commercially available defatted radish seed powder is dispersed in the buffer solution under stirring, and hydrolyzed at a constant temperature of 35° C. for 2 hours. After the hydrolysis reaction is completed, filter, collect the filtrate and the filter residue, and use the collected filter residue as a livestock feed additive after drying; for the collected filtrate, adjust the pH to 3 with a dilute HCl solution and then use a tubular centrifuge at 1200...

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Abstract

The invention discloses a method for preparing high-purity sulforaphene from carmine radish seeds, and relates to a method and a technology for rapidly and steadily preparing sulforaphene from the carmine radish seeds. According to the method, the powder of skimmed carmine radish seeds sold in the market is taken as a raw material; through enzymolysis and centrifugation, a centrifugate is obtained; resin coarse separation and high performance liquid chromatography preparation separation are sequentially conducted on the obtained centrifugate, so that a sulforaphene preparation liquid is obtained; finally, through rapid alcohol-water displacement, concentration, and freeze-drying, a sulforaphene product of which the purity reaches 99% or higher is obtained. The method is simple to operate, efficient, and high in continuity, and has the advantages that solvents can be repeatedly recovered and utilized, resin can be recycled, the sulforaphene product is steady and high in purity, and the problems that sulforaphene is liable to be subjected to rearrangement or isomerization in water, and the sulforaphene product is difficult to preserve for a long time can be solved. The sulforaphene product prepared according to the method can be widely applied to the fields of health products and pharmaceuticals.

Description

1. Technical field [0001] This patent belongs to the field of preparation and stability research of radish, and in particular relates to a method for rapidly and stably preparing high-purity radish from rouge radish seeds. 2. Background technology [0002] Rathotin is a class of isothiocyanate substances obtained by hydrolyzing glucosinolates in cruciferous vegetables in myrosinase, and its scientific name is 1-isothiocyanate-4-methylsulfonyl-butane. Also known as sulforaphane, sulforaphane, soluble in water, methanol and chloroform, easily soluble in ether, insoluble in petroleum ether. Rathuanin is one of the strongest anti-cancer ingredients found in vegetables so far. It can induce the body to produce type II detoxification enzymes, prevent carcinogens from destroying the genetic factors of healthy cells, and has broad market prospects. [0003] Existing technology for preparing radishine, for example, the application number is 201110117510.3, and the title is "a kind o...

Claims

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Application Information

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IPC IPC(8): C07C331/22
Inventor 杨志雄刘庆海周小华王江蒋小琴赵春花石业新
Owner GUANGZHOU LIUSHUN BIO TEC CO LTD
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