Ionization mass spectrometric detection device and application thereof

A mass spectrometry detection and mass spectrometry technology, applied in the field of analysis, can solve the problems affecting the application of paper spray, etc., and achieve the effects of simple structure, easy installation, and expansion of application scope.

Inactive Publication Date: 2017-03-08
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Paper spray can realize real-time in-situ detection of samples on paper substrates, but the paper needs to be cut into a specific shape. However, it is difficult for

Method used

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  • Ionization mass spectrometric detection device and application thereof
  • Ionization mass spectrometric detection device and application thereof
  • Ionization mass spectrometric detection device and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Embodiment 1. Transmission dielectric barrier discharge mass spectrometry ionization device

[0037] Such as figure 1 As shown, the dielectric barrier discharge ion source provided by the present invention includes an insulating dielectric tube 1 and two external electrodes 2 and 3. Two external electrodes are wound around the insulating medium pipe with a middle distance of 5-20mm, and the two electrodes are connected to the power supply device4. One end of the insulating medium tube is a working gas inlet 5 and the other end is a plasma flame outlet 6. When testing the sample, drop the sample on the filter paper 7, place 7 between the plasma flame 6 and the mass spectrometer ion source, and place the test sample point 8 in front of the mass spectrometry ion source inlet 9. When performing sample detection, the filter paper with sample point 8 should be aligned and close to the mass spectrometry ion source inlet 9 to ensure that there is no gap between 8 and 9.

[0038] u...

Example Embodiment

[0046] Example 2. The present invention directly detects amino acids separated by paper chromatography

[0047] First use the drawing software to draw the paper chromatographic channel, such as Image 6 a, and print with Xerox 8580 wax jet printer. Bake in an oven at 120 degrees Celsius for 45 seconds to allow the wax to penetrate the other side of the paper.

[0048] Prepare an amino acid mixture containing 50mM glycine, 50mM proline and 50mM leucine.

[0049] Drop the mixed liquid into the paper chromatographic channel with a capillary, the position and size are as Image 6 The red dot in a.

[0050] The developing agent of paper chromatography is butanol: formic acid: water = 15:2.4:2.6, and the developing time is 15 minutes.

[0051] After the end, the paper is dried in an oven at 65°C, and then evenly sprayed with 0.5% ninhydrin solution, heated and dried with a hair dryer, the color is as Image 6 Shown in b. It shows that the paper chromatography has separated the three amin...

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Abstract

The invention discloses an ionization mass spectrometric detection device and application thereof. The device comprises an insulation dielectric pipe, an outer electrode, a power supply device and a mass spectrometer ion source, wherein the outer electrode is wound outside the insulation dielectric pipe; the power supply device is connected with the outer electrode; one end of the insulation dielectric pipe is a carrier gas inlet; the other end of the insulation dielectric pipe is a plasma flame outlet; the mass spectrometer ion source is provided with a mass spectrometer ion source sample feeding opening; the mass spectrometer ion source sample feeding opening is near the plasma flame outlet. The device can be used for performing in-situ detection on samples on paper; the paper cutting and the high pressure exertion are not needed; the good combination with various paper core piece analysis devices can be realized; the advantages of a label-free effect and high flux are realized. In addition, the structure of the device is simple; the device can be easily built; in addition, the device can be used at barometric pressure; high-flowing-speed carrier gas is not needed; heating is not needed; the miniaturization of the instrument can be easily realized. The device can be used for detecting small molecules, and can also be used for detecting polypeptide and protein; the application range of a dielectric barrier discharge iron source is greatly expanded.

Description

technical field [0001] The invention belongs to the field of analysis, and relates to an ionization mass spectrometry detection device and its application. Background technique [0002] Microfluidic Paper-based Analytical Devices (μPADs) is a new type of microfluidic technology developed in recent years. Compared with traditional silicon, polymer, glass and other materials, paper is low in cost, easy to manufacture, portable, and has good biocompatibility. Therefore, paper-based microfluidic chips are gradually being used as disposable analysis devices. It is used in research fields such as life sciences, chemistry, and drug screening. The commonly used detection methods for microfluidic paper chip analysis are optical detection and electrochemical detection, among which optical detection is the most commonly used detection method. Optical detection usually converts the component to be tested into a colored substance through a color reaction, which has the advantages of be...

Claims

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Application Information

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IPC IPC(8): G01N27/62
Inventor 张新荣赵瑶瑶张四纯
Owner TSINGHUA UNIV
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