Chemically modified electrode for detecting cefalexin and electrochemical measurement method for cefalexin

A technology of chemical modification and determination method, applied in the field of electrochemical detection, can solve problems such as health hazards, and achieve the effects of low cost, high sensitivity and good selectivity

Active Publication Date: 2015-09-02
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If people eat this animal-derived food containing antibiotic

Method used

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  • Chemically modified electrode for detecting cefalexin and electrochemical measurement method for cefalexin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of a chemically modified electrode for detecting cephalexin

[0027] (1) Preparation of colloidal gold solution

[0028] Weigh a certain amount of chitosan and dissolve it in 1.0% acetic acid solution to make 30mL of 2mg / mL solution, add 15mL of 10mmol / L chloroauric acid solution under electromagnetic stirring, stir for 30min, then drop by drop under stirring Add 6mL of newly prepared 0.1mol / L sodium borohydride aqueous solution, and continue to stir until the solution becomes transparent wine red (about 1.5h);

[0029] (2) Preparation of chemically modified electrodes

[0030] After the gold electrode was polished with polishing powders of different particle sizes, it was rinsed with water, then placed in 1:1 nitric acid solution, absolute ethanol and water in sequence, cleaned with an ultrasonic cleaner, and dried naturally. Add 3 μl of colloidal gold solution dropwise on the surface of the treated gold electrode, and after drying at room tempe...

Embodiment 2

[0031] Example 2 Preparation of a chemically modified electrode for detecting cephalexin

[0032] (1) Preparation of colloidal gold solution

[0033] Weigh a certain amount of chitosan and dissolve it in 2.0% acetic acid solution to make 40mL of 3mg / mL solution, add 10mL of 20mmol / L chloroauric acid solution under electromagnetic stirring, stir for 40min, and then drop by drop under stirring Add 5mL of newly prepared 0.2mol / L sodium borohydride aqueous solution, and continue to stir until the solution becomes transparent wine red (about 1.5h);

[0034] (2) Preparation of chemically modified electrodes

[0035] After the gold electrode was polished with polishing powders of different particle sizes, it was rinsed with water, then placed in 1:1 nitric acid solution, absolute ethanol and water in sequence, cleaned with an ultrasonic cleaner, and dried naturally. Add 3 μl of colloidal gold solution dropwise on the surface of the treated gold electrode, and after drying at room t...

Embodiment 3

[0036]Example 3 Preparation of a chemically modified electrode for detecting cephalexin

[0037] (1) Preparation of colloidal gold solution

[0038] Weigh a certain amount of chitosan and dissolve it in 0.05% acetic acid solution to make 20mL of 1mg / mL solution, add 20mL of 5mmol / L chloroauric acid solution under electromagnetic stirring, stir for 40min, then drop by drop under stirring Add 10mL of newly prepared 0.05mol / L sodium borohydride aqueous solution, and continue to stir until the solution becomes transparent wine red (about 1.5h);

[0039] (2) Preparation of chemically modified electrodes

[0040] After the gold electrode was polished with polishing powders of different particle sizes, it was rinsed with water, then placed in 1:1 nitric acid solution, absolute ethanol and water in sequence, cleaned with an ultrasonic cleaner, and dried naturally. Add 3 μl of colloidal gold solution dropwise on the surface of the treated gold electrode, and after drying at room temp...

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Abstract

The invention relates to the technical field of electrochemical detection and discloses a chemically modified electrode for detecting cefalexin and an electrochemical measurement method for the cefalexin. The chemically modified electrode is prepared through steps that adding colloidal gold solution to a clean gold electrode surface, drying, soaking in hydrochloric acid solution with cysteine, self-assembling at 1 to 6 degrees centigrade, taking out, and using water to wash to clean. The electrochemical detection method includes steps that adding copper sulfate solution to sample solution to be measured, hydrolyzing to obtain hydrolysate, using the chemically modified electrode as a working electrode, and using a square wave voltammetry to measure the concentration of surplus copper ions in the hydrolysate to indirectly measure the content of the cefalexin in the sample. The chemically modified electrode is high in sensitivity, fast, convenient, low in cost and the like for measuring the cefalexin.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to a chemically modified electrode for detecting cephalexin and an electrochemical determination method for cephalexin. Background technique [0002] Cefalexin is a broad-spectrum antibiotic drug synthesized in the 1960s and belongs to the first generation of cephalosporins. It improves the penetration ability of the cell wall of Gram-negative bacteria and reduces the affinity of binding proteins. The antibacterial spectrum of cephalexin is the same as that of broad-spectrum penicillin, and it has strong antibacterial activity against Staphylococcus pneumoniae, Streptococcus pneumoniae, Salmonella and Neisseria producing or not producing penicillinase. It is mainly used for infections caused by Gram-positive bacteria and negative bacteria, and has a good bactericidal effect on penicillin-resistant fungi. [0003] Cephalosporins have been widely used in medicine an...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/26
Inventor 赵晓娟黄桂颖白卫东陈海光刘功良李艺勤
Owner ZHONGKAI UNIV OF AGRI & ENG
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