Biological prevention and control medicament for evergestis extimalis scopoli larvae, as well as preparation method and application method of biological prevention and control medicament

A technology for biological control of the fennel borer and biological control, applied in botany equipment and methods, insecticides, biocides, etc., can solve the problems of low drug efficacy, affecting crops, pollution-free green production, etc., and achieves the method simple effect

Inactive Publication Date: 2015-09-09
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the control of plant diseases and insect pests mainly relies on the control of chemical pesticides, which have low efficacy and long residual period.
And due to the large-scale application of chemical pesticides, air, water, soil and food are polluted, and poisonous substances accumulate in livestock and human bodies, causing poisoning, etc.
Moreover, the massive investment and long-term use of chemical pesticides have brought about many problems, such as the resistance of pests and pathogenic bacteria, the generation of residues in agricultural products, and the increase of residues in animals that feed on these crops due to bioaccumulation, affecting crops. pollution-free green production

Method used

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  • Biological prevention and control medicament for evergestis extimalis scopoli larvae, as well as preparation method and application method of biological prevention and control medicament
  • Biological prevention and control medicament for evergestis extimalis scopoli larvae, as well as preparation method and application method of biological prevention and control medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of culture medium: the components of culture medium are: peptone 8g; beef extract 2g; sodium chloride 3g; distilled water 1.0L; pH=6.0, sterilized, cooled for later use;

[0025] Absorb 1mL of liquid medium with a sterile pipette, drop it into the ampoule bottle, and shake gently to dissolve the Pseudomonas syringae cells in the ampoule bottle in a suspended state;

[0026] Ice nucleation active bacteria culture: suck the bacterial suspension, transplant it into 50mL medium, cultivate to logarithmic growth phase at 60% relative humidity and 27°C, and dry to obtain ice nucleation active bacteria - Pseudomonas syringae.

Embodiment 2

[0028] Preparation of culture medium: the components of culture medium are: peptone 8g; beef extract 2g; sodium chloride 3g; distilled water 1.0L; pH=6.0, sterilized, cooled for later use;

[0029] Draw 1 mL of liquid medium with a sterile pipette, drop it into the ampoule bottle, and shake gently to dissolve the Erwinia bacteria in the ampoule bottle in a suspended state;

[0030] Ice nucleation active bacteria culture: suck the bacterial suspension, transplant it into 50mL medium, cultivate to logarithmic growth phase at 60% relative humidity and 27°C, and dry to obtain ice nucleation active bacteria - Erwinia.

Embodiment 3

[0032] Preparation of culture medium: the components of culture medium are: peptone 14g; beef extract 6g; sodium chloride 10g; distilled water 1.0L; pH=8.0, sterilized, cooled for later use;

[0033] Absorb 0.5mL of liquid medium with a sterile pipette, drop it into the ampoule bottle, and shake gently to dissolve the Pseudomonas syringae cells in the ampoule bottle in a suspended state;

[0034] Ice nucleation active bacteria culture: suck the bacterial suspension, transplant it into 200mL medium, cultivate to logarithmic growth phase at 80% relative humidity and 30°C, and dry to obtain ice nucleation active bacteria - Pseudomonas syringae.

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Abstract

The invention relates to the technical field of agricultural pest control, in particular to a biological prevention and control medicament for controlling evergestis extimalis scopoli, as well as a preparation method and an application method of the biological prevention and control medicament. The biological prevention and control medicament adopts ice-nucleation active bacteria as an effective active ingredient, wherein the content of the ice-nucleation active bacteria is 1 to 90 percent of the total weight, and the ice-nucleation active bacteria are one or the mixture of Pseudomonassyringae and Erwiniapyrifoliae; Pseudomonassyringae as well as Erwiniapyrifoliae is 0.1 to 99.9 wt% of the mixture; the ice-nucleation active bacteria are made into missible oil, microemulsion, emulsion oil in water, an aqueous solution, suspension concentrate, an oil solution, oil miscible flowable concentrate, microcapsule suspension or water dispersible granules under the condition that additives are added or not added. The undercooling point of overwintering larvae of evergestis extimalis scopoli is raised by utilizing the ice-nucleation active bacteria, so that the overwintering larvae freeze to death or are injured due to cold at a relatively high temperature during an overwintering period, that is, the next year pest source is reduced. Therefore, the pollution-free, low-toxic and environment-friendly controlling purposes are realized, and the controlling effect is realized.

Description

technical field [0001] The invention relates to the technical field of agricultural disease and insect pest control, in particular to a biological control agent for preventing and controlling A. fennel, a preparation method and a use method thereof. Background technique [0002] Evergestisextimalis Scopli belongs to the family Lepidoptera, Pyralidida. Also known as rape horn borer, fennel borer, and rape borer, it is mainly distributed in northern areas such as Qinghai, Gansu, Ningxia, Inner Mongolia and Heilongjiang, and abroad in North Korea, Japan, the United States and Northwest Asia. Mainly harm fennel, beet, cabbage, rape, shepherd's purse, radish, cabbage, mustard and other plants. On spring rapeseed, it is mainly harmed by larvae digging through siliques and eating grains. In recent years, the harm of hornwort has shown a trend of "spreading from low altitudes to high altitudes in the Haidong rapeseed planting area of ​​Qinghai Province". [0003] The control of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P7/04A01G13/00
Inventor 来有鹏郭青云咸文荣王一鸣李秋荣陈海民
Owner QINGHAI UNIVERSITY
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