Biological prevention and control medicament for evergestis extimalis scopoli larvae, as well as preparation method and application method of biological prevention and control medicament
A technology for biological control of the fennel borer and biological control, applied in botany equipment and methods, insecticides, biocides, etc., can solve the problems of low drug efficacy, affecting crops, pollution-free green production, etc., and achieves the method simple effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] Preparation of culture medium: the components of culture medium are: peptone 8g; beef extract 2g; sodium chloride 3g; distilled water 1.0L; pH=6.0, sterilized, cooled for later use;
[0025] Absorb 1mL of liquid medium with a sterile pipette, drop it into the ampoule bottle, and shake gently to dissolve the Pseudomonas syringae cells in the ampoule bottle in a suspended state;
[0026] Ice nucleation active bacteria culture: suck the bacterial suspension, transplant it into 50mL medium, cultivate to logarithmic growth phase at 60% relative humidity and 27°C, and dry to obtain ice nucleation active bacteria - Pseudomonas syringae.
Embodiment 2
[0028] Preparation of culture medium: the components of culture medium are: peptone 8g; beef extract 2g; sodium chloride 3g; distilled water 1.0L; pH=6.0, sterilized, cooled for later use;
[0029] Draw 1 mL of liquid medium with a sterile pipette, drop it into the ampoule bottle, and shake gently to dissolve the Erwinia bacteria in the ampoule bottle in a suspended state;
[0030] Ice nucleation active bacteria culture: suck the bacterial suspension, transplant it into 50mL medium, cultivate to logarithmic growth phase at 60% relative humidity and 27°C, and dry to obtain ice nucleation active bacteria - Erwinia.
Embodiment 3
[0032] Preparation of culture medium: the components of culture medium are: peptone 14g; beef extract 6g; sodium chloride 10g; distilled water 1.0L; pH=8.0, sterilized, cooled for later use;
[0033] Absorb 0.5mL of liquid medium with a sterile pipette, drop it into the ampoule bottle, and shake gently to dissolve the Pseudomonas syringae cells in the ampoule bottle in a suspended state;
[0034] Ice nucleation active bacteria culture: suck the bacterial suspension, transplant it into 200mL medium, cultivate to logarithmic growth phase at 80% relative humidity and 30°C, and dry to obtain ice nucleation active bacteria - Pseudomonas syringae.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com