Method for marking protein by carboxyl microsphere

A technology for labeling proteins and microspheres, which is applied in the direction of material inspection products, measuring devices, instruments, etc., can solve problems such as harsh reaction conditions, wasting antibodies, and affecting coupling efficiency, achieving high-efficiency coupling, reducing dosage, and avoiding hydrolysis Effect
CN104897904AInactive Publication Date: 2015-09-09BIOSINO BIO TECH & SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BIOSINO BIO TECH & SCI
Publication Date
2015-09-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for marking protein by a carboxyl microsphere, belonging to the field of preparation of immunologic diagnosis kits. According to invention, in a system for marking the protein by the carboxyl microsphere, according to the content of carboxyls on the surface of the microsphere, the molar ratio of cross-linking agents EDC to -COOH is set to be (0.8-2) to 1, and the molar ratio of EDC to NHS or Sulfo to NHS is (1-3) to 1, so that the optimal ratio of the number of the carboxyls on the surface of the microsphere to the number of activated carboxyls is achieved; meanwhile, the pH is regulated, so that the reaction system is rapidly converted from condition beneficial for activation into state beneficial for antibody connection, the step of centrifugalization or tangential flow filtering is saved, and finally, the purposes of simplifying marking steps, saving cost, and marking protein by the carboxyl microsphere efficiently are achieved. The method provided by the invention can be applied to preparation of a detection kit taking the latex microsphere or the magnetic microsphere as a carrier, and has good market application prospect and economic value.
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Description

technical field

[0001] The invention relates to the technical field of preparation of immunodiagnostic kits, in particular to a novel, low-cost and easy-to-operate carboxyl microsphere-labeled protein method. Background technique

[0002] Nano-microspheres are widely used in in vitro diagnostic reagents, which greatly improve the sensitivity of the reagents and can detect substances with minimal sample content. Nano-microspheres are used as carriers in diagnostic reagents with uniform size. The surface of the microspheres is coated with protein molecules such as antibodies and antigens. There are two methods for protein molecules to adsorb onto nano-microspheres: physical adsorption and chemical coupling. Physical adsorption is a relatively traditional and effective method, but it also has the disadvantages of being sensitive to system changes and poor stability; chemical coupling can avoid these defects. In a narrow sense, chemical coupling refers to the use of commerciall...

Claims

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