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High-density liquid strain and fermenting method thereof

A technology of high-density liquid and fermentation method, which is applied in the field of high-density liquid bacteria and its fermentation. It can solve the problems of multiplication algebra gap, shorten the age of bacteria, and small diameter of mycelium balls, so as to achieve uniform distribution and shorten the age of bacteria. and the effect of the difference in the number of multiplication algebras and the small diameter of mycelium balls

Active Publication Date: 2015-09-23
XINJIANG XIPU BIOLOGICAL SCI & TECH
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem solved by the present invention is to overcome the defects of the existing Pleurotus eryngii liquid strains prepared by aerated fermentation, and the scientific compounding in the medium of each stage of the strain cultivation process can improve the cold resistance, heat stress and immunity of the strains. The active Chinese herbal medicine extract and the adaptive raw material sawdust powder are added inoculated in stages and fermented at variable temperatures to shorten the age and proliferation algebra gap of the strains, and the coated chitosan is added in a timely manner to adjust the viscosity of the fermentation medium. Cultivate a Pleurotus eryngii liquid strain with strong fermented hyphae, high activity, small mycelium ball diameter, high density, uniform distribution, and short fermentation period

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 raw material preparation

[0052] 1. Preparation of Chinese herbal medicine extract:

[0053] The preparation method of the Chinese herbal medicine extract comprises the steps of: weighing 30 parts of astragalus, 30 parts of dried ginger, 30 parts of tangerine peel, 25 parts of bezoar, 25 parts of paeonol, 20 parts of licorice, and 18 parts of anemarrhena in parts by weight. 18 parts of honeysuckle, 17 parts of Houttuynia cordata, 15 parts of red peony, 15 parts of scutellaria baicalensis, 10 parts of Chuanxiong, and 8 parts of angelica; put them in an ultrasonic cleaner filled with 0.2% sodium bicarbonate solution for 12 minutes at 200W and 30KHz, Drain, crush the above-mentioned Chinese herbal medicine to a particle size of less than 2 mm, put it in a container and mix evenly and add 5 times the weight of water to obtain a mixed material, control the temperature at 80°C for 3 hours, then lower the temperature to 45°C, and adjust the pH value with lactic a...

Embodiment 2

[0061] A method for fermenting high-density liquid strains, comprising the steps of:

[0062] 1) Take the preserved Pleurotus eryngii slant strain 0.2cm 2 Inoculate 2 pieces of the strain block of the above-mentioned bacteria on the plate medium for activation, and culture at a constant temperature of 25°C for 10 days, and activate twice in this way to obtain plate seeds;

[0063] 2) Use a puncher with a diameter of 0.5 cm to intercept the flat seed 0.2 cm 2 3 pieces of the strain block were inserted into a 250mL Erlenmeyer flask, and the amount of liquid seed culture medium was 100mL. They were cultured at a constant temperature of 25°C for 2 days, then placed in a constant temperature shaker, and shaken at 25°C and 100r / min for 3 days. liquid seeds;

[0064] 3) The liquid seeds were inoculated in a 500 mL shake flask with an inoculum amount of 10%, and the liquid volume of the shake flask culture medium was 250 mL, and cultured at 25° C. and 150 r / min at a constant tempera...

Embodiment 3

[0075] A method for fermenting high-density liquid strains, comprising the steps of:

[0076] Step 1) to 4) with embodiment 2;

[0077] 5) First inoculate the seeds of the seed tank with a 5% inoculum amount in a 30L fermenter, and ferment at 30°C with a ventilation rate of 3L / min for 12 hours at a constant temperature of 15L, and then cool down to 18°C ​​at a rate of 0.3°C / min. ℃, add 8g coated chitosan and 900mL seed tank seeds to the fermenter, ferment at constant temperature for 10h under the condition of ventilation rate 6L / min, and finally raise the temperature to 24℃ at the rate of 0.2℃ / min, and then increase the temperature to 24℃ at the rate of ventilation rate 5L / min Fermentation at constant temperature for 45 hours under certain conditions to obtain high-density liquid strains;

[0078] Described plate medium composition is the same as embodiment 2;

[0079] The liquid seed medium consists of: glucose 30g / L, yeast extract juice 5g / L, peptone 2g / L, agar 2g / L, Chine...

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Abstract

The invention discloses a high-density liquid strain and a fermenting method thereof, and belongs to the technical field of culture of pleurotus eryngii. According to the preparation method, Chinese herbal medicine extracts which can improve the cold resistance, thermal stress, immunity and hybrid bacteria resistance of the strain are scientifically composited in the culture medium at each stage of liquid seeds, seeds in a shake flask, seeds in a seed tank, and liquid strain in a fermenting tank; meanwhile, sawdust which has the raw material adaptability and also has the function of a defoaming agent is scientifically composited; in addition, the mode of supplemental inoculating and sectional adjustable temperature fermenting is carried out, in order to reduce the cell age of the strain and the difference in the number of generation of proliferation; chitosan which is coated and has the special function is added by a flowing manner at a proper time so as to culture the liquid strain which is strong in fermenting hypha, high in activity, small in mycelium pellet diameter, large in density, and uniform to distribute; the density of the mycelium pellet is 4.5 to 5.5x105 units / L; the diameter of the mycelium pellet is 0.5 to 0.7m; the dry weight of the mycelium pellet is 90 to 100g / L; the germination costs 4 to 5 hours; the exopolysaccharide is 3.0 to 3.5g / L.

Description

technical field [0001] The invention relates to the technical field of Pleurotus eryngii cultivation, in particular to a high-density liquid strain and a fermentation method thereof. Background technique [0002] Pleurotus eryngii (PleurotuseryngiiQuel.), also known as Pleurotus eryngii, has the advantages of thick flesh and crisp texture, especially its dense, firm, milky white and refreshing stipe. It is called "king of oyster mushrooms" and "scallop mushrooms". , with almond fragrance and abalone taste, suitable for fresh-keeping and processing, and is deeply loved by people. It is rich in nutrients, rich in protein, carbohydrates, vitamins and minerals such as calcium, magnesium, copper, zinc, etc., which can improve human immune function. It has the effects of anti-cancer, lowering blood fat, moisturizing the stomach and beautifying the human body, and has extremely high cultivation value and commercial value. [0003] At present, the cultivation of Pleurotus eryngii a...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
CPCA01G18/00C05B17/00C05D5/00C05G3/00
Inventor 李建树
Owner XINJIANG XIPU BIOLOGICAL SCI & TECH
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