Application of flavonoid theaflavanoside III in preventing plant nematode diseases

A technology of flavonoids and plant nematodes, applied in the direction of application, nematicides, plant growth regulators, etc., to achieve the effect of easy degradation

Active Publication Date: 2015-09-23
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The flavonoids have not been studied on the effect on nematodes

Method used

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  • Application of flavonoid theaflavanoside III in preventing plant nematode diseases
  • Application of flavonoid theaflavanoside III in preventing plant nematode diseases
  • Application of flavonoid theaflavanoside III in preventing plant nematode diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022]Embodiment 1: Preparation of the flavonoid compound theaflavanoside III described in compound (1)

[0023] The residue (tea seed cake) of Camellia oleifera seed is pulverized with a plant pulverizer, passed through a 40-mesh sieve, soaked in 95% edible ethanol with 8 times the sample size (w / v), and placed in a cool place. Stir frequently. After 5-7 days, filter, add ethanol to the residue and soak, repeat twice, and combine the filtrate. The ethanol filtrate was decompressed on a rotary evaporator, concentrated below 45°C, and then extracted three times with petroleum ether to remove excess oil in the material. Add 3% hydrogen peroxide to the solution after extraction and degreasing, adjust the pH value to 10, put it in a water bath at 60° C., and decolorize it for 90 minutes. The decolorized solution was concentrated to a paste on a rotary evaporator, and dried in vacuo to obtain a crude extract. The crude extract is used as the material, and the components are sepa...

Embodiment 2

[0025] Example 2: Determination of Egg Hatching Inhibitory Activity to Plant Nematodes

[0026] Bioassay of egg hatching inhibitory activity: use 24-well biological culture plate (draw a good line at the bottom), add 0.5mL of 1mg / mL medicinal solution to each hole (the final concentration of the solution is 0.83% after adding egg grains), then add 0.1 Egg fluid with a mL concentration of 1000 grains / mL was repeated 4 times for each treatment, and sterile water was used as a control, placed in a 25°C incubator, and the hatched second-instar larvae were recorded under an inverted microscope on the 3rd, 5th, and 7th day respectively Number, and calculate the egg hatch inhibition rate:

[0027]

[0028] Egg hatching inhibitory activity test shows that, with 0.83mg / ml concentration treatment M. Table 1.

[0029] Table 1 Inhibitory activity of theaflavanoside Ⅲ on egg hatching of M. incognita

[0030]

[0031] Note: 1) The data format in the table is: Represents the ave...

Embodiment 3

[0033] Embodiment 3: Determination of poisonous activity to plant nematodes

[0034] Weigh 0.05g of compound (1) flavonoid theaflavanoside III, and dissolve it in 10mL of sterilized pure water (the sample is first dissolved with a small amount of sterilized water at about 50°C, and then adjusted to 10mL), to obtain a concentration of 5mg / mL concentration, and then diluted with sterilized pure water to the required gradient concentration. Use a 24-well biological culture plate, add 0.5mL of each concentration gradient drug solution to each well, and then add 0.5mL of second-instar larva suspension (about 100-200), and set 4 repetitions for each treatment. As a control, put in a 25°C incubator, observe and count at 24h, 36h, 48h, 72h, and 96h (24h after resuscitating with clear water), record the nematode life and death data under an inverted microscope, and calculate the corrected mortality:

[0035]

[0036]

[0037] The bioassay results showed that the lethal medium c...

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Abstract

The invention relates to an application of flavonoid theaflavanoside III in preventing plant nematode diseases. The structure of the flavonoid theaflavanoside III is shown as the formula (I). The flavonoid theaflavanoside III has high poisonous activity for heterodera, root-knot nematode, pratylenchus coffeae and aphelenchoides besseyi and especially has high toxicity for heterodera. Field experiment of camellia seed cake powder containing the flavonoid theaflavanoside III shows that the preventive effect for the soybean heterodera disease is equivalent to that of conventional chemical nematicide lythidathion. (See specifications).

Description

technical field [0001] The present invention relates to flavonoid compound theaflavanoside III (molecular formula C 33 h 42 o 19 ) in the prevention and treatment of plant nematode diseases. Background technique [0002] Plant nematodes are one of the important pathogenic organisms of plants. With the development of agriculture towards industrialization and base, long-term large-scale planting of single crops, continuous cropping all year round and the improvement of land reuse rate, the number of nematodes in the soil accumulates rapidly, and nematode diseases The problem is becoming more and more prominent. In northern China, wheat cyst nematodes (Heterodera. avenarea, H. filipjevi) and soybean cyst nematodes (Heterodera glycines) are common and harmful, causing significant losses and impacts on the production of wheat and soybeans in my country (Peng Deliang, Zhang Dongsheng, Qi Shuhua, Chen Pinsan. Optimum investigation period and method of cereal cyst nematodes in wh...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P5/00
Inventor 文艳华彭德良孙玉红彭焕廖金铃孔令安
Owner SOUTH CHINA AGRI UNIV
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