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A kind of indocyanine green composite nanoparticle and its preparation method and application

A technology of composite nanoparticles and indocyanine green, which is applied in the field of nanomaterials, can solve the problems of lack of tumor cell targeting specificity, limit the application of indocyanine green, and short cycle time, so as to avoid decomposition and clearance in the body, increase Target recognition effect, effect of improving stability

Active Publication Date: 2017-12-08
ZHUHAI INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, indocyanine green will quickly aggregate in polar solvents, and its stability is poor. It will be quickly cleared in plasma, and the circulation time in the body is short. At the same time, indocyanine green also lacks tumor cell targeting specificity. These Defects greatly limit the application of indocyanine green

Method used

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  • A kind of indocyanine green composite nanoparticle and its preparation method and application
  • A kind of indocyanine green composite nanoparticle and its preparation method and application
  • A kind of indocyanine green composite nanoparticle and its preparation method and application

Examples

Experimental program
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Embodiment 1

[0079] A preparation method of indocyanine green composite nanoparticles, comprising the following steps:

[0080] (1) Extract cancer cell membrane: take cancer cells, add hypotonic buffer solution, homogeneously break up, and collect the precipitate by centrifugation several times, that is, cancer cell membrane;

[0081] Human breast cancer cells (MCF-7) were inoculated in T-175 cell culture flasks, cultured with DMEM medium, and added with 10% FBS, 1% penicillin, and 1% streptomycin. After the cells were confluent, The old medium was discarded, digested with EDTA, centrifuged and washed 3 times with PBS to obtain the target cancer cells;

[0082] Add 20mM Tris-HCl hypotonic buffer solution with pH=7.5 to the washed MCF-7 cells, the hypotonic buffer solution also contains 10mM KCl, 2mM MgCl 2 , 1.0 μg / ml protease inhibitor, use a Dounce homogenizer to homogenize the cells for 20 times, centrifuge at a low speed of 3200g for 5 minutes, save the supernatant, and add hypotonic ...

Embodiment 2

[0090] A preparation method of indocyanine green composite nanoparticles, comprising the following steps:

[0091] (1) Extract cancer cell membrane: the steps are the same as in Example 1;

[0092] (2) Preparation of vesicle structure: the ratio of the extracted cancer cell membrane to DSPE-PEG (where the molecular weight of PEG is 400) is 3×10 8 : 180 μg was weighed, and dissolved in 1ml, 4% ethanol, and ultrasonic cell disruptor was used to ultrasonicate for 5min at a frequency of 20kHz and a power of 35W under an ice bath to obtain the first mixed solution;

[0093] (3) Preparation of a spherical inner core: dissolving indocyanine green (ICG) in ethanol to obtain a 0.5 mg / ml ICG solution, dissolving polylactic acid-glycolic acid copolymer (PLGA) in acetonitrile to obtain a 0.5 mg / ml PLGA solution , take 1.5ml of ICG solution, and ultrasonicate it, while adding 1ml of PLGA solution dropwise into 1.5ml of ICG solution, ultrasonic time is 2min, to obtain the second mixed solu...

Embodiment 3

[0096] A preparation method of indocyanine green composite nanoparticles, comprising the following steps:

[0097] (1) Extract cancer cell membrane: the steps are the same as in Example 1;

[0098] (2) Preparation of vesicle structure: the ratio of the extracted cancer cell membrane to DSPE-PEG (wherein the molecular weight of PEG is 20000) is 9×10 8 : 180 μg was weighed, and dissolved in 1ml, 4% ethanol, and ultrasonic cell disruptor was used to ultrasonicate for 5min at a frequency of 20kHz and a power of 35W under an ice bath to obtain the first mixed solution;

[0099] (3) Preparation of a spherical inner core: dissolving indocyanine green (ICG) in ethanol to obtain a 0.5 mg / ml ICG solution, dissolving polylactic-co-glycolic acid (PLGA) in acetonitrile to obtain a 2 mg / ml PLGA solution, Take 1.5ml of ICG solution and ultrasonicate it, while adding 1ml of PLGA solution dropwise into 1.5ml of ICG solution, ultrasonic time is 2min, to obtain the second mixed solution;

[01...

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Abstract

The invention provides an indocyanine green composite nanoparticle, the indocyanine green composite nanoparticle comprises indocyanine green, hydrophobic polymer, polyethylene glycol derivatized phospholipid and cancer cell membrane, the indole The cyanine green is surrounded by the hydrophobic polymer and forms a spherical structure with the hydrophobic polymer, and the polyethylene glycol derivatized phospholipid is interspersed in the cancer cell membrane and forms a vesicle with the cancer cell membrane structure, the spheroid structure is covered in the vesicle structure. The present invention also provides a preparation method and application of indocyanine green composite nanoparticles. When the indocyanine green composite nanoparticles are applied, the composite nanoparticles have high-efficiency targeting to tumors, and the indocyanine green composite nanoparticles Green is not easy to gather and has good stability.

Description

technical field [0001] The invention belongs to the field of nanometer materials, in particular to an indocyanine green composite nanoparticle and its preparation method and application. Background technique [0002] Indocyanine Green (ICG) is a hydrogen tricarbonate dye, its maximum absorption wavelength and maximum emission wavelength are in the near-infrared region of 740nm and 800nm, and its fluorescence penetration is strong. It is a reagent approved by FDA (FDA) for human medical imaging and diagnosis, and it is used as a photothermal preparation for clinical hyperthermia. However, indocyanine green will quickly aggregate in polar solvents, and its stability is poor. It will be quickly cleared in plasma, and the circulation time in the body is short. At the same time, indocyanine green also lacks tumor cell targeting specificity. These The defect greatly limits the application of indocyanine green. Contents of the invention [0003] In order to solve the above prob...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/46A61K47/24A61K9/14A61P35/00
Inventor 蔡林涛陈泽郑明彬赵鹏飞罗震宇龚萍
Owner ZHUHAI INST OF ADVANCED TECH
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