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Medium series and tissue culture method for rapid propagation and seedling growth of Dendrobium nobile

A Dendrobium nobile, proliferation medium technology, applied in the rapid propagation medium series of Dendrobium nobile and the field of tissue culture, can solve the problems of long seedling growth period, high cost and low germination rate in test tubes

Active Publication Date: 2017-09-29
临沧市云瑞堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of endosperm, the seeds need to symbiosis with fungi to germinate, and the germination rate is very low (less than 5%) under natural conditions
[0003] At present, there have been related reports on the tissue culture research of Dendrobium nobile, but the high cost of tissue culture and the long cycle of test tube seedlings are the bottlenecks restricting the industrial production of Dendrobium nobile

Method used

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  • Medium series and tissue culture method for rapid propagation and seedling growth of Dendrobium nobile
  • Medium series and tissue culture method for rapid propagation and seedling growth of Dendrobium nobile
  • Medium series and tissue culture method for rapid propagation and seedling growth of Dendrobium nobile

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Preparation of liquid A: Weigh 17.5g of ammonium nitrate, 19.5g of potassium nitrate, 4.3g of magnesium sulfate, and 2.4g of potassium dihydrogen phosphate, then add to 1L of water and mix to dissolve.

[0041] Preparation of liquid B: Weigh 4.6g of calcium chloride, then put it into 1L of water and mix to dissolve.

[0042] Prepare liquid C: weigh 0.4g of glycine, 0.05g of vitamin B1, 0.15g of nicotinic acid and 0.1g of pyridoxine hydrochloride VB6 and add them into 600ml of water, mix and dissolve to obtain mixed solution I, then weigh 440g of inositol and mix with 400ml of water to dissolve To obtain the mixed solution II, just mix the mixed solution I and the mixed solution II.

[0043] Preparation of liquid D: Weigh 5.59g of ferrous sulfate and 7.47g of disodium ethylenediaminetetraacetate, dissolve them in water, and then mix to prepare a 1L solution.

[0044] Prepare L liquid: Weigh 19.9g / L manganese sulfate, 9.2g / L zinc sulfate, 6.6g / L boric acid, 0.83g / L potas...

Embodiment 2

[0065] The content and preparation method of liquid A, liquid B, liquid C, liquid D, liquid L, liquid M1 and liquid M2 are the same as in Example 1.

[0066] Preparation of seeding medium: Weigh 1000ml of liquid A, 1000ml of liquid B, 100ml of liquid C, 100ml of liquid D, 20ml of liquid L and 8ml of M1 and mix well, then add 380g of sucrose, 72g of agar, 34g of carbon powder With 1900g crushed bananas, add 10 liters of water and heat at high temperature, then mix at 80°C, bottle, sterilize in an autoclave, and cool.

[0067] Preparation of proliferation medium: Weigh 1000ml of liquid A, 1000ml of liquid B, 100ml of liquid C, 100ml of liquid D, 20ml of liquid L, 8ml of liquid M1 and 5ml of liquid M2, then add 380g of sucrose, 72g of Agar, 34g of carbon powder and 1900g of crushed bananas, plus 10 liters of water, heated at high temperature, then mixed at 80°C, bottled, sterilized in an autoclave, and cooled.

[0068] Preparation of strong seedling medium: Weigh 1000 ml of liqu...

Embodiment 3

[0084] The content and preparation method of liquid A, liquid B, liquid C, liquid D, liquid L, liquid M1 and liquid M2 are the same as in Example 1.

[0085] Preparation of seeding medium: Weigh 1000ml of A liquid, 1000ml of B liquid, 100ml of C liquid, 100ml of D liquid, 20ml of L liquid and 8ml of M1 liquid, then add 420g of sucrose, 76g of agar, 38g of carbon powder With 2100g of crushed apples, add 10 liters of water and heat at high temperature, then mix at 80°C, bottle, sterilize in an autoclave, and cool.

[0086] Preparation of proliferation medium: Weigh 1000ml of liquid A, 1000ml of liquid B, 100ml of liquid C, 100ml of liquid D, 20ml of liquid L, 8ml of liquid M1 and 5ml of liquid M2, then add 420g of sucrose, 76g of Agar, 38g of carbon powder and 2100g of crushed apples, plus 10 liters of water, heated at high temperature, then mixed at 80°C, bottled, sterilized in an autoclave, and cooled.

[0087] Preparation of strong seedling medium: weigh 1000 ml of liquid A,...

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Abstract

The invention provides a culture medium series and a tissue culture method for the rapid propagation and seedling formation of Dendrobium nobile. The medium series include seeding medium, proliferation medium, strong shoot medium, differentiated shoot medium and rooting medium. In the tissue culture method of the present invention, at first the seeds are cultured in the germination medium, and light treatment is directly carried out without dark culture treatment. After the seeds germinate to form protocorms, callus is induced in the protocorm proliferation induction medium, and the callus is further Protocorm-like is formed; the protocorm-like is differentiated to form young seedlings; the young seedlings are moved to a strong seedling medium, and after the small seedlings grow into large seedlings; the large seedlings are cultivated in the rooting medium to become Dendrobium nobile adult seedlings. The invention can shorten the cultivation time of Dendrobium nobile, not only is easy to operate, but also is not limited by time and place, the continuous division and multiplication of protocorm-like can ensure annual production, and is convenient for large-scale industrial production and application.

Description

technical field [0001] The invention relates to a breeding method of medicinal plant seedlings, in particular to a rapid propagation medium series of Dendrobium nobile and a tissue culture method. Background technique [0002] Dendrobium nobile, also known as: nobile stone, flat gold hairpin, flat yellow grass, flat grass, is a perennial herb, named after the hairpin on the ancient head. The seeds of Dendrobium nobile are as fine as dust, and one capsule contains as many as 1 million seeds. Due to the lack of endosperm, the seeds need to symbiosis with fungi to germinate, and the germination rate is very low (less than 5%) under natural conditions. The natural reproductive capacity of Dendrobium nobile is extremely low, and conventional reproduction is difficult. Over the years, the amount of collection of Dendrobium nobile is far greater than its growth, and the natural reproduction of wild Dendrobium nobile is on the verge of extinction. In order to develop artificial cu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈茂云
Owner 临沧市云瑞堂生物科技有限公司
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