Three-carbon electrode electrochemiluminescence base fabric micro-fluidic chip and preparation method and application thereof

A microfluidic chip and electrochemistry technology, applied in the field of microfluidic analysis, can solve the problems that have not been reported, and achieve the effects of small sample consumption, good stability and high detection sensitivity

Active Publication Date: 2015-09-30
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, until now, the sensing method combining electrochemiluminescence assay with bucky microfluidic chip has not been reported.

Method used

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  • Three-carbon electrode electrochemiluminescence base fabric micro-fluidic chip and preparation method and application thereof
  • Three-carbon electrode electrochemiluminescence base fabric micro-fluidic chip and preparation method and application thereof
  • Three-carbon electrode electrochemiluminescence base fabric micro-fluidic chip and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A method for preparing a three-carbon electrode electrochemiluminescent cloth-based microfluidic chip, comprising the following steps:

[0057] (1) Use Adobe Illustrator CS5 drawing software to design the shape of the reaction cell (crone shape), the shape of the reaction cell is as follows figure 1 as shown, figure 1 The middle white area is the reaction pool (also called hydrophilic area, hydrophilic pool), and then made into a 300-mesh reaction pool screen; use Adobe Illustrator CS5 drawing software to design electrode patterns, such as figure 2 Shown: the working electrode is circular, the counter electrode and the reference electrode are semi-circular, and then made into a 300-mesh electrode mesh.

[0058] (2) Cut a piece of cloth with a width of 80 mm and a length of 110 mm. The cloth is white plain cotton cloth with about 10.7 mg per square centimeter and 96 threads per inch (about 14.3 mg per meter of thread).

[0059] (3) Place the cloth under the screen of ...

Embodiment 2

[0063] The three-carbon electrode electrochemiluminescence cloth-based microfluidic chip of Example 1 quantitatively detects TPA, and the detection process is as follows:

[0064] (1) First fix the chip on the hydrophobic polyethylene bracket, and put the bracket into the dark box, align the working electrode in the chip reaction pool with the macro lens (model M1614-MP) of the CCD (model MC15), and adjust the CCD Relevant parameters to make the image clearest, the imaging detection device such as Figure 4 shown.

[0065] (2) Add 11 μL of detection working solution into the reaction pool, wait for 3 to 5 seconds, start the CCD automatic imaging function to collect electrochemiluminescence images, and then start the potentiostat (model CHI 1242B) to trigger electrochemiluminescence and record the luminescence imaging data.

[0066] (3) The imaging data were further analyzed and processed by Matlab R2012a (MathWorks company, USA) and Origin 7.0 (Microcal Software Inc., Newark...

Embodiment 3

[0074] The three-carbon electrode electrochemiluminescence cloth-based microfluidic chip of Example 1 quantitatively detects H 2 o 2 , its detection process is the same as in Example 2.

[0075] detect H 2 o 2 The working solution is composed of a concentration of 5mM luminol solution (configured by 0.1M NaOH solution) and different concentrations of H 2 o 2 solution (diluted with 0.1M NaOH solution in 30% H 2 o 2 obtained) by mixing equal volumes.

[0076] First verify the quantitative detection of H 2 o 2 Feasibility, set the H in the working solution 2 o 2 The concentration was 0, 0.5 and 2mM, and the scanning potential and rate of cyclic voltammetry were -0.5~1V and 100mV / s, respectively.

[0077] Figure 8 The solid line is 0mM H 2 o 2 Luminescence curve, dotted line and dashed line are 0.5mM and 2mM H 2 o 2 Glow curve. Comparing the luminous value of the dotted line and the virtual curve, it can be seen that: with the H 2 o 2 Concentration increased fr...

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Abstract

The invention discloses a three-carbon electrode electrochemiluminescence base fabric micro-fluidic chip and a preparation method and application thereof. The preparation method of the chip comprises the following steps of designing the shape of a reaction tank and patterns of electrodes, and then obtaining a screen of the reaction tank and a screen of the electrodes, wherein the electrodes are three-carbon electrodes and include the working electrode, the counter electrode and the reference electrode; conducting screen printing of the shape of the reaction tank on a fabric, conducting screen printing of the patterns of the electrodes on the same fabric, and conducting airing to obtain the three-carbon electrode electrochemiluminescence base fabric micro-fluidic chip. The electrode screen printing processing method is applied onto the base fabric micro-fluidic chip for the first time, the processing method has the advantage that no expensive or complex instruments are needed, and has the other important advantage that the base fabric micro-fluidic chips with the reaction tanks and the electrodes can be obtained in batches. The electrodes are the three-carbon electrodes, and compared with a traditional three-electrode system, the manufacturing cost is low; usage for one time can be achieved, and no complex polishing pretreatment is needed.

Description

technical field [0001] The invention belongs to the field of microfluidic analysis, and in particular relates to a three-carbon electrode electrochemiluminescent cloth-based microfluidic chip, its preparation method and application. Background technique [0002] Since 2011, microfluidic bucky analysis devices (or bucky microfluidic chips) have been developed to a certain extent. Compared with the microfluidic paper-based analysis devices developed earlier, the cloth-based microfluidic chip has the following distinct advantages: (1) The cloth material is very cheap and can be produced in almost every place in the world, It belongs to renewable and recyclable resources; (2) biochemical materials such as reaction reagents are easy to modify cloth materials by coating, printing and dyeing or dip-dyeing; (3) cloth can be used to filter or separate samples; (4) cloth is A biodegradable material that can be discarded directly or safely disposed of by incineration; (5) the cloth ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N21/76
Inventor 章春笋管文荣刘敏
Owner SOUTH CHINA NORMAL UNIVERSITY
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