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Culture medium for detecting lactobacillaceae in traditional Chinese medicine oral fluid preparation and detection method

A liquid preparation and detection method technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of no lactobacillus detection method and invalid lactic acid bacteria, so as to prevent large-scale pollution incidents and promote health To develop and ensure the effect of medication safety

Active Publication Date: 2015-09-30
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

④ Although potassium sorbate and other bacteriostatic agents are added to the product, this ingredient only has an inhibitory effect on mold, yeast and aerobic bacteria, and is almost ineffective against lactic acid bacteria
[0006] Checking the relevant literature at home and abroad, there is no detection method for Lactobacillus contaminated with traditional Chinese medicine oral liquid

Method used

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  • Culture medium for detecting lactobacillaceae in traditional Chinese medicine oral fluid preparation and detection method
  • Culture medium for detecting lactobacillaceae in traditional Chinese medicine oral fluid preparation and detection method
  • Culture medium for detecting lactobacillaceae in traditional Chinese medicine oral fluid preparation and detection method

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Experimental program
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Embodiment 1

[0025] Bacillus subtilis (Bacillus subtilis) [CMCC (B) 63501], Staphylococcus aureus (Staphylococcus aureus) [CMCC (B) 26003], Escherichia coli (Escherichia coli) [CMCC (B) 44102], sporogenous Clostridium sporogenes [CMCC (B) 64941], Candida albicans [CMCC (F) 98001], and Aspergillus niger [CMCC (F) 98003] were used as validation strains, representing Common bacillus, Gram-positive cocci, Gram-negative bacilli, molds and yeasts in the environment are used to verify the inhibitory ability of the two lactobacillus culture mediums described in the present invention to miscellaneous bacteria; using Lactobacillus plantarum Plant subspecies (Lactobacillus plantarum) [CICC20718] and Lactobacillus buchneri (Lactobacillus buchneri) [CICC 20293] were used as strains for verification to verify the effectiveness of the detection method for Lactobacillus in oral liquid preparations of traditional Chinese medicine, and to check the growth-promoting ability and indicating ability, etc.

[0...

Embodiment 2

[0036] The invention belongs to the qualitative detection of lactobacilli in products, and refers to the minimum number of microorganisms that can be detected in products under set test conditions. The detection limit corresponds to the number of microorganisms contained in the sample before dilution or incubation, rather than the number of microorganisms contained in the test solution at a certain step in the detection process. The detection method of the present invention has a detection limit of less than 5 cfu / 100mL for lactobacilli.

[0037] In order to verify the detection limit of the method of the present invention, the standard method and the method of the present invention should be used to test the positive samples containing lactobacilli respectively, so as to compare the difference between the two methods with the detection results. At present, there is no standard method for the detection of Lactobacillus in oral liquid preparations of traditional Chinese medicin...

Embodiment 3

[0050] In order to ensure the accuracy and reliability of the inspection results, when establishing an inspection method for a certain microorganism, the verification of the inspection method should be carried out to confirm that the method adopted is suitable for the inspection of the product. If the composition of the product or the original inspection conditions are changed, which may affect the accuracy of the inspection results, the inspection method should be re-validated. When verifying the method described in the present invention, Lactobacillus plantarum (Lactobacillus plantarum) [CICC 20718] was used as the verification strain. Embodiment 2 Taking a commercially available heat-clearing traditional Chinese medicine oral liquid as an example, specification: 100mL / bottle, introduces the operation method of the present invention.

[0051] 1. Bacterial solution preparation Take a fresh culture of Lactobacillus plantarum subsp. plantarum and inoculate it into the liquid cu...

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Abstract

The invention relates to a detection method for detecting whether lactobacillaceae in a traditional Chinese medicine oral fluid preparation is polluted or not, as well as a culture medium for detecting lactobacillaceae in the traditional Chinese medicine oral fluid preparation. The culture medium comprises two parts: (1) a lactobacillaceae enrichment medium which comprises 5.0 g of peptone, 4.0 g of yeast extract powder, 10.0 g of glucose, 10.0 g of cane sugar, 1.0 g of dipotassium phosphate, 0.3 g of magnesium sulphate, 5.0 g of polysorbate 80, 3 g of potassium sorbate and 1000 mL of water; (2) a lactobacillaceae agar medium which comprises 10.0 g of peptone, 5.0 g of glucose, 5.0 g of cane sugar, 3.0 g of sodium chloride, 5.0 g of yeast extract powder, 1.0 g of L-cystine, 1.0 g of dipotassium phosphate, 17 g of agar, 3 g of potassium sorbate and 1000 mL of water. Through the adoption of the culture medium and the detection method provided by the invention, a key area in a traditional Chinese medicine oral fluid preparation production line can be continuously monitored, so that hidden pollution dangers in the production line can be detected and removed in time, and large-scale pollution accidents can be prevented.

Description

technical field [0001] The present invention relates to the field of drug microorganism detection, in particular to a detection method for detecting whether lactobacilli are contaminated in oral liquid preparations of traditional Chinese medicines, and a culture medium for detecting lactobacilli in oral liquid preparations of traditional Chinese medicines Background technique [0002] Traditional Chinese medicine oral liquid preparation is based on traditional Chinese medicine decoction, extracting active ingredients from the original medicinal materials, adding flavoring agents, antibacterial agents, etc., and then making it through standing, filtering, and sterilizing. It has small dosage and is easy to take. , rapid onset, easy to accept taste and other advantages, it is widely used in clinical practice. However, the raw materials of traditional Chinese medicine oral liquid are mostly natural animal and plant ingredients, with high protein content, and the preparations ar...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 丁勃国明凌霄刘广桢徐晓洁胡文宏
Owner SHANDONG INST FOR FOOD & DRUG CONTROL