Preparation method of rhus verniciflua stoke seed polysaccharide with antioxidant activity
An anti-oxidant activity and the technology of sumac seed, which is applied in the field of the preparation of sumac seed polysaccharide and polysaccharide extract from sumac seed meal, can solve the problem that the preparation method of sumac seed polysaccharide has not been reported at home and abroad, resources have not been fully utilized, and no patent publication has been seen and other problems, to achieve good anti-oxidation effect, improve comprehensive utilization rate, and enhance the effect of added value
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Embodiment 1
[0028] Example 1 Determination of polysaccharide content by phenol sulfuric acid method
[0029] Glucose standard curve preparation: Weigh 0.01g of glucose, dissolve it in a 50 mL volumetric flask with distilled water, take eight 10 mL test tube labels (blank, 1, 2, ... 7), draw standard glucose solutions 0.1, 0.2, 0.3, 0.4, 0.5, 0.7, 0.9 mL, respectively placed in No. 1-7 test tubes, add distilled water to a final volume of 1.0 mL, then add 1.0 mL of 5% (w / w) phenol, shake well, slowly add concentrated sulfuric acid 3.5 mL, quickly oscillate and shake well, after 25 minutes of color development at room temperature, pipette 250 μL into a 96-well plate, three wells for each sample, measure the absorbance at 490 nm, and take the average value, distilled water is used as a blank control. Draw the standard curve with the glucose concentration as the abscissa and the absorbance value as the ordinate; the linear equation of the standard curve is y=0.0081x-0.0139 (R 2 =0.9992), indi...
Embodiment 2
[0032] Dry and pulverize the wild sumac seed meal, add petroleum ether at a mass ratio of 1:5 (g:mL), heat and reflux at 40°C for 3 times, 10h each time, filter, combine the filtrates, evaporate and recover under reduced pressure at 40°C Solvent and filter residue were dried under reduced pressure to obtain defatted seed meal; the defatted seed meal was added with distilled water at a mass volume ratio of 1:5 (g:mL), extracted at room temperature for 3 times, each time for 10 hours, filtered, and the filtrates were combined, Concentrate under reduced pressure at 70°C, and then dry under vacuum to obtain the crude extract; add absolute ethanol to the concentrated solution at a volume ratio of 1:10 (v / v), carry out alcohol precipitation, and centrifuge to obtain a precipitate, which is then subjected to ether, Acetone and ethanol were washed 3 times, and then dried to obtain the crude polysaccharide of sumac seed meal. The content of the crude polysaccharide was determined by the...
Embodiment 3
[0034]Dry and crush the sumac seed meal, add n-hexane at a mass ratio of 1:30 (g:mL), heat and reflux at 70°C for 2 extractions, each time for 2 hours, filter, combine the filtrates, evaporate and recover under reduced pressure at 60°C The solvent and the filter residue were dried under reduced pressure to obtain the defatted seed meal; the defatted seed meal was added with distilled water at a mass volume ratio of 1:30 (g:mL), heated and refluxed for extraction at an extraction temperature of 50-90°C, and extracted twice. Filtrate for 2 hours each time, combine the filtrates, concentrate under reduced pressure at 50°C, and then freeze-dry to obtain the crude extract; add absolute ethanol to the concentrated solution at a volume ratio of 1:20 (v / v) for alcohol precipitation , centrifuged to obtain a precipitate, and the precipitate was washed twice with ether, acetone and ethanol, and then dried, which was the crude polysaccharide of sumac seed meal. The content of the crude po...
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